Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response

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art_PEREIRA_Melittin_induces_in_vitro_death_of_Leishmania_Leishmania_2016.PDF (798.14 KB)
Citações na Scopus
24
Tipo de produção
article
Data de publicação
2016
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
BIOMED CENTRAL LTD
Autores
PEREIRA, Andreia Vieira
BARROS, Gustavo de
TEMPONE, Andre Gustavo
ORSI, Ricardo de Oliveira
SANTOS, Lucilene Delazari dos
CALVI, Sueli
FERREIRA JR., Rui Seabra
PIMENTA, Daniel Carvalho
BARRAVIERA, Benedito
Citação
JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES, v.22, article ID 1, 8p, 2016
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods: Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti-and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results: Promastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 mu g.mL(-1) and 1.4 mu g.mL(-1), respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 mu g.mL(-1). Uninfected macrophages treated with melittin increased the production of IL-10, TNF-alpha, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-alpha, NO and H2O2. Conclusions: The results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect.
Palavras-chave
Melittin, Apis mellifera, Leishmania, Leishmaniasis, Peptides, Toxins, Antiparasitic, Cytokines
URI
https://observatorio.fm.usp.br/handle/OPI/13635
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Coleções
Artigos e Materiais de Revistas Científicas - IMT