Characterization of topoisomerase II alpha and minichromosome maintenance protein 2 expression in anal carcinoma

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art_SCAPULATEMPO-NETO_Characterization_of_topoisomerase_II_alpha_and_minichromosome_maintenance_2017.PDF (704.75 KB)
Citações na Scopus
8
Tipo de produção
article
Data de publicação
2017
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPANDIDOS PUBL LTD
Autores
SCAPULATEMPO-NETO, Cristovam
VEO, Carlos
FREGNANI, Jose Humberto T. G.
LORENZI, Adriana
MAFRA, Allini
MELANI, Armando G. F.
LOAIZA, Edgar Antonio Aleman
Citação
ONCOLOGY LETTERS, v.13, n.3, p.1891-1898, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The present study aimed to ascertain the significance of topoisomerase II alpha (TOP2A) and minichromosome maintenance protein (MCM) 2 expression in anal carcinoma. A total of 75 anal lesions were retrieved from the files of the Department of Pathology of Barretos Cancer Hospital (Barretos, Brazil) in order to verify the human papillomavirus (HPV) statuses of these lesions and characterize the immunohistochemical expression levels of TOP2A and MCM2 in anal carcinoma, as these are important markers for cervical HPV-induced lesions; their expression was also compared with respect to p16 and Ki-67. The vast majority of the cases tested positive for HPV16 (84%); 1 case tested positive for both HPV16 and HPV18. Positive HPV16 status was more frequent in early stages than in advanced stages (P=0.008). Positive immunohistochemical reactivity for MCM2 and TOP2A protein was observed in 71.6 and 100% of cases, respectively. Positive reactivity for p16 was significantly associated (P=0.001) with histological grade, and was more commonly expressed in squamous cell carcinoma than adenocarcinomas. HPV16 was strongly associated with positive p16 protein expression (76.6%). However, the high expression of Ki-67 combined with the high expression of p16 was predominantly observed in Stage III-IV cases. MCM2, TOP2A, p16 and Ki-67 exhibited intense positive staining in the anal lesions, indicating that these markers were significantly and constantly expressed in anal carcinoma.
Palavras-chave
anal carcinoma, p16, Ki-67, minichromosome maintenance protein 2, topoisomerase II alpha, human papillomavirus
URI
https://observatorio.fm.usp.br/handle/OPI/20362
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Coleções
Artigos e Materiais de Revistas Científicas - LIM/14
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/52
Artigos e Materiais de Revistas Científicas - ODS/03