RHD and RHCE genotyping by next-generation sequencing is an effective strategy to identify molecular variants within sickle cell disease patients
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Citações na Scopus
33
Tipo de produção
article
Data de publicação
2017
Título da Revista
ISSN da Revista
Título do Volume
Editora
ACADEMIC PRESS INC ELSEVIER SCIENCE
Autores
VIEIRA, Juliana B.
VARUZZA, Leonardo
CHINOCA, Karen Ziza
Citação
BLOOD CELLS MOLECULES AND DISEASES, v.65, p.8-15, 2017
Resumo
Background: The complexity of Rh genetic variation among sickle cell disease (SCD) patients is high. Conventional molecular assays cannot identify all genetic variants already described for the RH locus as well as foresee novel alleles. Sequencing RHD and RHCE is indicated to broaden the search for Rh genetic variants. Aims: To standardize the Next Generation Sequencing (NGS) strategy to assertively identify Rh genetic variants among SCD patients with serologic suspicion of Rh variants and evaluate if it can improve the transfusion support. Methods: Thirty-five SCD patients with unexplained Rh antibodies were enrolled. A NGS-based strategy was developed to genotype RHD and RHCE using gene-specific primers. Genotype and serological data were compared. Results: Data obtained from the NGS-based assay were gene-specific. Ten and 25 variant RHD and RHCE alleles were identified, respectively. Among all cases of unexplained Rh antibodies, 62% had been inaccurately classified by serological analysis and, of these, 73.1% were considered as relevant, as were associated with increased risk of hemolytic reactions and shortage of units suitable for transfusion. Conclusion: The NGS assay designed to genotype RH coding regions was effective and accurate in identifying variants. The proposed strategy clarified the Rh phenotype of most patients, improving transfusion support.
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Referências
- Casas J, 2015, TRANSFUSION, V55, P1388, DOI 10.1111/trf.12987
- Chiu RWK, 2001, CLIN CHEM, V47, P667
- Chou ST, 2013, BLOOD, V122, P1062, DOI 10.1182/blood-2013-03-490623
- Chou ST, 2012, BRIT J HAEMATOL, V159, P394, DOI 10.1111/bjh.12061
- De Santis D, 2013, INT J IMMUNOGENET, V40, P72, DOI 10.1111/iji.12024
- Dezan V.B.O.M.R., 2016, ISBT SCI SER INT J I, V11, P132
- Dinardo Carla Luana, 2013, Rev. Bras. Hematol. Hemoter., V35, P414, DOI 10.5581/1516-8484.20130123
- Fichou Y, 2016, VOX SANG, V111, P418, DOI 10.1111/vox.12432
- Fichou Y, 2014, BRIT J HAEMATOL, V167, P554, DOI 10.1111/bjh.13084
- Hendrickson JE, 2007, BLOOD, V110, P2736, DOI 10.1182/blood-2007-03-083105
- Johnsen JM, 2015, HEMATOL-AM SOC HEMAT, P168
- Legler TJ, 2001, TRANSFUSION MED, V11, P383, DOI 10.1046/j.1365-3148.2001.00327.x
- Maaskant-van Wijk PA, 1998, TRANSFUSION, V38, P1015, DOI 10.1046/j.1537-2995.1998.38111299056309.x
- Noizat-Pirenne F, 2011, TRANSFUS CLIN BIOL, V18, P527, DOI 10.1016/j.tracli.2011.09.001
- Percy MJ, 2012, AM J HEMATOL, V87, P439, DOI 10.1002/ajh.23123
- Reid ME, 2014, BLOOD CELL MOL DIS, V52, P195, DOI 10.1016/j.bcmd.2013.11.003
- Singleton BK, 2000, BLOOD, V95, P12
- Sippert E, 2015, BLOOD TRANSFUS-ITALY, V13, P72, DOI 10.2450/2014.0324-13
- Stabentheiner S, 2011, VOX SANG, V100, P381, DOI 10.1111/j.1423-0410.2010.01444.x
- Vichinsky EP, 2001, TRANSFUSION, V41, P1086, DOI 10.1046/j.1537-2995.2001.41091086.x
- Wagner FF, 1998, BLOOD, V91, P2157
- Yazdanbakhsh K, 2012, BLOOD, V120, P528, DOI 10.1182/blood-2011-11-327361
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