Rapid antigen detection test for respiratory syncytial virus diagnosis as a diagnostic tool

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art_MESQUITA_Rapid_antigen_detection_test_for_respiratory_syncytial_virus_2017.PDF (918.06 KB)
Citações na Scopus
13
Tipo de produção
article
Data de publicação
2017
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
SOC BRASIL PEDIATRIA
Autores
MESQUITA, Flavio da Silva
OLIVEIRA, Danielle Bruna Leal de
CREMA, Daniela
PINEZ, Celia Miranda Nunes
COLMANETTI, Thais Cristina
THOMAZELLI, Luciano Matsumia
MARTINEZ, Marina Baquerizo
BOTOSSO, Viviane Fongaro
Citação
JORNAL DE PEDIATRIA, v.93, n.3, p.246-252, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Objective: The aim of this study was to evaluate the QuickVue (R) RSV Test Kit (QUIDEL Corp, CA, USA) as a screening tool for respiratory syncytial virus in children with acute respiratory disease in comparison with the indirect immunofluorescence assay as gold standard. In Brazil, rapid antigen detection tests for respiratory syncytial virus are not routinely utilized as a diagnostic tool, except for the diagnosis of dengue and influenza. Methods: The authors retrospectively analyzed 486 nasopharyngeal aspirate samples from children under age 5 with acute respiratory infection, between December 2013 and August 2014, the samples were analyzed by indirect immunofluorescence assay and QuickVue (R) RSV Test kit. Samples with discordant results were analyzed by real time PCR and nucleotide sequencing. Results: From 313 positive samples by immunofluorescence assays, 282 (90%) were also positive by the rapid antigen detection test, two were positive only by rapid antigen detection test, 33 were positive only by immunofluorescence assays, and 171 were positive by both methods. The 35 samples with discordant results were analyzed by real time PCR; the two samples positive only by rapid antigen detection test and the five positive only by immunofluorescence assays RSV Test and viral load or specific strain. The QuickVue (R) RSV Test showed sensitivity of 90%, specificity of 98.8%, predictive positive value of 99.3%, and negative predictive value of 94.6%, with accuracy of 93.2% and agreement kappa index of 0.85 in comparison to immunofluorescence assay. Conclusions: This study demonstrated that the QuickVue RSV Test Kit can be effective in early detection of Respiratory syncytial virus in nasopharyngeal aspirate and is reliable for use as a diagnostic tool in pediatrics. (C) 2016 Sociedade Brasileira de Pediatria.
Palavras-chave
Respiratory-viruses, Respiratory syncytial virus - RSV, Rapid antigen detection test - RADT
URI
https://observatorio.fm.usp.br/handle/OPI/21383
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - ODS/03