Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/2171
Title: | Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy |
Authors: | FAIAO-FLORES, Fernanda; COELHO, Paulo Rogerio Pinto; ARRUDA-NETO, Joao Dias Toledo; MARIA-ENGLER, Silvya Stuchi; TIAGO, Manoela; CAPELOZZI, Vera Luiza; GIORGI, Ricardo Rodrigues; MARIA, Durvanei Augusto |
Citation: | PLOS ONE, v.8, n.3, article ID e59639, 12p, 2013 |
Abstract: | Boron neutron capture therapy (BNCT) is a binary treatment involving selective accumulation of boron carriers in a tumor followed by irradiation with a thermal or epithermal neutron beam. The neutron capture reaction with a boron-10 nucleus yields high linear energy transfer (LET) particles, alpha and Li-7, with a range of 5 to 9 mu m. These particles can only travel very short distances and release their damaging energy directly into the cells containing the boron compound. We aimed to evaluate proliferation, apoptosis and extracellular matrix (ECM) modifications of B16F10 melanoma and normal human melanocytes after BNCT. The amounts of soluble collagen and Hsp47, indicating collagen synthesis in the ECM, as well as the cellular markers of apoptosis, were investigated. BNCT decreased proliferation, altered the ECM by decreasing collagen synthesis and induced apoptosis by regulating Bcl-2/Bax in melanoma. Additionally, BNCT also increased the levels of TNF receptor and the cleaved caspases 3, 7, 8 and 9 in melanoma. These results suggest that multiple pathways related to cell death and cell cycle arrest are involved in the treatment of melanoma by BNCT. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/25 Artigos e Materiais de Revistas Científicas - ODS/03 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_FAIAO-FLORES_Apoptosis_through_Bcl_2_Bax_and_Cleaved_Caspase_2013.PDF | publishedVersion (English) | 2 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.