Detection of sputum cofilin-1 as indicator of malignancy
Carregando...
Citações na Scopus
8
Tipo de produção
article
Data de publicação
2018
Título da Revista
ISSN da Revista
Título do Volume
Editora
Associação Brasileira de Divulgação Científica
Autores
DE SÁ, V.K.
LEÃO, P.S.
FARHAT, C.
FABRO, A.T.
REIS, R.M.
Citação
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.51, n.8, p.e7138, 2018
Resumo
Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of sputum CFL1 in discriminating cancer patients from cancer-free patients and healthy volunteers were 0.78 and 0.69, respectively. CFL1 at a cut-off value of 415.25 pg/mL showed sensitivity/specificity of 0.80/0.70 in differentiating between healthy volunteers and cancer patients. Sputum CFL1 was also able to identify cancer-free patients from patients with lung cancer. The AUC was 0.70 and, at a cut-off point ≥662.63 pg/mL, we obtained 60% sensitivity and 54% specificity. Logistic regression analysis controlled for tobacco history, histologic types, and N stage showed that cancer cell-associated CFL1 was an independent predictor of death. Smoker patients with squamous cell carcinoma, lymph node metastasis and sputum CFL1>1.475 pg/mL showed augmented chance of death, suggesting lung cancer aggressiveness. CFL1 presented diagnostic value in detecting lung cancer and was associated to tumor aggressiveness.
Palavras-chave
Lung cancer, Cofilin-1, Sputum, Liquid-biopsy, Metastases, Biomarker
Referências
- Aberle DR, 2013, J CLIN ONCOL, V31, P1002, DOI 10.1200/JCO.2012.43.3110
- Arber S, 1998, NATURE, V393, P805
- FONTANA RS, 1991, CANCER-AM CANCER SOC, V67, P1155, DOI 10.1002/1097-0142(19910215)67:4+<1155::AID-CNCR2820671509>3.0.CO;2-0
- Gadgeel SM, 2016, ADV EXP MED BIOL, V890, P203, DOI 10.1007/978-3-319-24932-2_11
- Hotulainen P, 2005, MOL BIOL CELL, V16, P649, DOI 10.1091/mbc.E04-07-0555
- Howington JA, 2013, CHEST, V143, pE278, DOI 10.1378/chest.12-2359
- Kelloff GJ, 2012, NAT REV DRUG DISCOV, V11, P201, DOI 10.1038/nrd3651
- Ma H, 2013, STAT MED, V32, P3449, DOI 10.1002/sim.5777
- Melamed MR, 2000, CANCER, V89, P2356, DOI 10.1002/1097-0142(20001201)89:11+<2356::AID-CNCR8>3.0.CO;2-Z
- Melo SA, 2015, NATURE, V523, P177, DOI 10.1038/nature14581
- Ono S, 2007, INT REV CYTOL, V258, P1, DOI 10.1016/S0074-7696(07)58001-0
- Rangel M.P., 2015, Braz J Med Biol Res, V48, P557, DOI 10.1590/1414-431X20144300
- TOCKMAN MS, 1986, CHEST, V89, pS324, DOI 10.1378/chest.89.4_Supplement.324S-a
- Wang W, 2006, J CELL BIOL, V173, P395, DOI 10.1083/jcb.200510115
- Wang WG, 2007, NAT REV CANCER, V7, P429, DOI 10.1038/nrc2148
- Wistuba II, 2002, ONCOGENE, V21, P7298, DOI 10.1038/sj.onc.1205806
- Yan B, 2010, ACTA HISTOCHEM, V112, P101, DOI 10.1016/j.acthis.2008.07.007
- [郑育举 Zheng Yuju], 2013, [南方医科大学学报, Journal of Southern Medical University], V33, P1551
- Zhu BB, 2006, CANCER RES, V66, P8640, DOI 10.1158/0008-5472.CAN-06-1443
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/09
Carregar mais Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/09