Optimization of von Willebrand factor multimer analysis in vertical mini-gel electrophoresis systems: A rapid procedure

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art_THOMAZINI_Optimization_of_von_Willebrand_factor_multimer_analysis_in_2019.PDF (2.26 MB)
Citações na Scopus
7
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PERGAMON-ELSEVIER SCIENCE LTD
Autores
Citação
THROMBOSIS RESEARCH, v.175, p.76-83, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Von Willebrand disease (VWD) is a common cause of bleeding worldwide. Analysis of von Willebrand factor (VWF) multimer distribution (VWF:MD) is essential to properly classify and treat different types of VWD, and it is performed using a SDS agarose gel electrophoresis followed by Western blotting, a handmade technique that demands days to be completed and requires skillful execution. Aiming both to facilitate gel production and to shorten the preparation time, we developed an uncomplicated technique to provide agility in the analysis of VWF:MD, so that it can be easily accomplished in the routine practice of hemostasis laboratories. On that account, we used a commercial vertical mini-gel electrophoresis system for SDS-PAGE and a semi-dry transfer system, which allowed us to analyze VWF:MD of various samples in a period shorter than 12 h. This technique differentiated VWF:MD in human and animal plasmas under normal, congenital and acquired (experimental envenomation by Bothrops jararaca snake) conditions. This optimized method is cheap, rapid, reproducible, easy to be performed, and uses electrophoresis and Western blotting systems available in most laboratories. All these advantages encourage hemostasis professionals to use it in their routine practices. In order to facilitate the setup and accomplishment of the whole procedure step by step, videos were appended to the article.
Palavras-chave
ADAMTS13, Bleeding, Diagnosis, Electrophoresis, Proteolysis
URI
https://observatorio.fm.usp.br/handle/OPI/31256
Referências
  1. Bowen DJ, 2007, THROMB HAEMOSTASIS, V97, P1051, DOI 10.1160/TH06-12-0726
  2. Budde U, 2002, SEMIN THROMB HEMOST, V28, P173, DOI 10.1055/s-2002-27820
  3. Budde U, 2015, EXPERT REV HEMATOL, V8, P799, DOI 10.1586/17474086.2015.1060854
  4. Castaman G, 2016, SEMIN THROMB HEMOST, V42, P478, DOI 10.1055/s-0036-1579638
  5. COUNTS RB, 1978, J CLIN INVEST, V62, P702, DOI 10.1172/JCI109178
  6. DALTON RG, 1988, THROMB RES, V50, P345, DOI 10.1016/0049-3848(88)90237-X
  7. DONG ZG, 1994, J BIOL CHEM, V269, P6753
  8. Favaloro EJ, 2017, HAEMOPHILIA, V23, pE373, DOI 10.1111/hae.13261
  9. Favaloro EJ, 2016, PATHOLOGY, V48, P303, DOI 10.1016/j.pathol.2016.03.001
  10. Favaloro EJ, 2011, BLOOD COAGUL FIBRIN, V22, P553, DOI 10.1097/MBC.0b013e32834a7e01
  11. FEDERICI AB, 1989, BRIT J HAEMATOL, V73, P93, DOI 10.1111/j.1365-2141.1989.tb00226.x
  12. Federici AB, 2009, BLOOD, V113, P526, DOI 10.1182/blood-2008-04-152280
  13. Fischer BE, 1998, BIOCHEM J, V331, P483, DOI 10.1042/bj3310483
  14. FOWLER WE, 1985, J CLIN INVEST, V76, P1491, DOI 10.1172/JCI112129
  15. FRETTO LJ, 1986, J BIOL CHEM, V261, P5679
  16. Gorr TA, 2015, PROTEOM CLIN APPL, V9, P396, DOI 10.1002/prca.201400118
  17. GRALNICK HR, 1987, AM J HEMATOL, V24, P259, DOI 10.1002/ajh.2830240305
  18. Haberichter SL, 2008, BLOOD, V111, P4979, DOI 10.1182/blood-2007-09-110940
  19. Haberichter SL, 2006, BLOOD, V108, P3344, DOI 10.1182/blood-2006-04-015065
  20. Harlow E, 1988, ANTIBODIES LAB MANUA, P726
  21. HOYER LW, 1980, BLOOD, V55, P1056
  22. Johnstone IB, 1997, J VET DIAGN INVEST, V9, P314, DOI 10.1177/104063879700900316
  23. Just S, 2017, SEMIN THROMB HEMOST, V43, P75, DOI 10.1055/s-0036-1592164
  24. KAMIGUTI AS, 1991, TOXICON, V29, P961, DOI 10.1016/0041-0101(91)90079-7
  25. Kurien BT, 2003, J IMMUNOL METHODS, V274, P1, DOI 10.1016/S0022-1759(02)00523-9
  26. Laffan M., 2011, DACIE LEWIS PRACTICA, P393
  27. Lenting PJ, 2015, BLOOD, V125, P2019, DOI 10.1182/blood-2014-06-528406
  28. Lopes AA, 2002, BRAZ J MED BIOL RES, V35, P1259, DOI 10.1590/S0100-879X2002001100002
  29. MARUYAMA M, 1990, THROMB HAEMOSTASIS, V63, P449
  30. MILLER JL, 1991, P NATL ACAD SCI USA, V88, P4761, DOI 10.1073/pnas.88.11.4761
  31. Ng C, 2015, BLOOD, V125, P2029, DOI 10.1182/blood-2014-08-528398
  32. Nichols T. C., 2010, THROMBOSIS, V2010
  33. Ott HW, 2010, AM J CLIN PATHOL, V133, P322, DOI 10.1309/AJCPZSBTD2BWOMVL
  34. Pasotti F, 2013, ELECTROPHORESIS, V34, P818, DOI 10.1002/elps.201200538
  35. Patton WF, 2000, ELECTROPHORESIS, V21, P1123, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1123::AID-ELPS1123>3.3.CO;2-5
  36. Perutelli P, 1997, HAEMATOLOGICA, V82, P510
  37. Pruthi RK, 2010, THROMB RES, V126, P543, DOI 10.1016/j.thromres.2010.09.015
  38. Pukac LA, 1997, BIOTECHNIQUES, V23, P385, DOI 10.2144/97233bm08
  39. RUGGERI ZM, 1981, BLOOD, V57, P1140
  40. RUGGERI ZM, 1980, J CLIN INVEST, V65, P1318, DOI 10.1172/JCI109795
  41. Sadler JE, 2006, J THROMB HAEMOST, V4, P2103, DOI 10.1111/j.1538-7836.2006.02146.x
  42. Sadler J. E., 2013, HEMOSTASIS THROMBOSI, P670
  43. SANDERS WE, 1988, LAB INVEST, V59, P443
  44. Sanders YV, 2015, BLOOD, V125, P3006, DOI 10.1182/blood-2014-09-603241
  45. Santoro ML, 2004, THROMB HAEMOSTASIS, V92, P369, DOI 10.1160/TH04-02-0120
  46. Schneppenheim R, 2001, BLOOD, V97, P2059, DOI 10.1182/blood.V97.7.2059
  47. Seidel H., 2017, RES PRACT THROMB SS1, V1, P717
  48. Serrano SMT, 2007, FEBS J, V274, P3611, DOI 10.1111/j.1742-4658.2007.05895.x
  49. Smejkal GB, 2003, ELECTROPHORESIS, V24, P582, DOI 10.1002/elps.200390068
  50. Springer TA, 2014, BLOOD, V124, P1412, DOI 10.1182/blood-2014-05-378638
  51. Studt JD, 2001, AM J CLIN PATHOL, V116, P567
  52. TAKAHASHI H, 1984, BLOOD, V64, P1254
  53. Vincentelli A, 2003, NEW ENGL J MED, V349, P343, DOI 10.1056/NEJMoa022831
  54. von Willebrand EA, 1999, HAEMOPHILIA, V5, P223
  55. Weiss DR, 2008, THROMB HAEMOSTASIS, V100, P949, DOI 10.1160/TH08-06-0403
  56. World Federation of Hemophilia, 2016, REP ANN GLOB SURV 20, P64
  57. Yamashita KM, 2014, PLOS NEGLECT TROP D, V8, DOI 10.1371/journal.pntd.0002814
  58. ZIMMERMAN TS, 1986, J CLIN INVEST, V77, P947, DOI 10.1172/JCI112394

Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/31