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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTEDDER, Richard S.
dc.contributor.authorDICKS, Steve
dc.contributor.authorIJAZ, Samreen
dc.contributor.authorSOUZA, Nathalia Caroline Santiago de
dc.contributor.authorPAULA, Anderson Vincente de
dc.contributor.authorLEVY, Flavia
dc.contributor.authorMEDIALDEA-CARRERA, Raquel
dc.contributor.authorLEVI, Jose Eduardo
dc.contributor.authorPANNUTI, Claudio S.
dc.contributor.authorSEQUEIRA, Patricia Carvalho de
dc.contributor.authorBROWN, David W. G.
dc.contributor.authorLUMB, Ines Ushiro
dc.date.accessioned2019-11-06T18:47:42Z-
dc.date.available2019-11-06T18:47:42Z-
dc.date.issued2019
dc.identifier.citationPLOS ONE, v.14, n.8, article ID e0215708, 16p, 2019
dc.identifier.issn1932-6203
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/34032-
dc.description.abstractThe accurate diagnosis and seroprevalence investigations of Zika virus (ZKV) infections remain complex due to cross reactivity with other flaviviruses. Two assay formats, both using labelled Zika virus NS1 antigen as a revealing agent (a double antigen binding assay, DABA, and an immunoglobulin Ig capture assay, G capture) were initially developed and compared with the indirect EuroimmunZ assay for the detection of anti-Zika antibody. Of 147 pre-Zika period serum samples, 39 (27%) were reactive in the EuroimmunZ or the DABA assays, 28 sera concordantly so. Such false reactivity was influenced by the serotype of Dengue virus (DV) to which individuals had been exposed to. Thus, of sera from patients undergoing secondary Dengue virus infection of known serotype, 91%, 45% and 28% of Dengue virus serotype 2, 3 and 4 respectively were reactive in one or more of the three assays. A novel method of quenching false sero-reactivity was therefore developed for the DABA and G capture assays. Initial addition of a single homologous Dengue virus serotype 3 NS1Ag quench significantly ablated false reactivities in the pre-Zika period sera. An equipotent quadrivalent quench comprising homologous Dengue virus serotypes 1 to 4 NS1Ag was shown to be optimum yet retained sensitivity for the detection of specific anti-Zika antibody. Comparing DABA and G capture assays using quenched and unquenched conjugates in comparison with EuroimmunZ early in the course of PCR-confirmed infection indicated that a significant component of the apparent early anti-ZIKA antibody response is likely to be due to a Zika virus-driven anamnestic anti-Dengue virus response. The increased specificity provided by homologous antigen quenching is likely to provide a significant improvement in sero-diagnostics and to be of clinical value.eng
dc.description.sponsorshipMedical Research Council Zika Rapid Response award [MC_PC_15093]
dc.description.sponsorshipPublic Health England Pipeline fund [PLF 1819-115/RA]
dc.description.sponsorshipEuropean Union's horizon 2020 research and innovation programme [734857]
dc.description.sponsorshipZikaction
dc.description.sponsorshipZikaplan [734584]
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCEeng
dc.relation.ispartofPlos One
dc.rightsopenAccesseng
dc.subject.othertransmissioneng
dc.subject.otherinfectioneng
dc.subject.otherdiseaseeng
dc.subject.otherassayeng
dc.titleModulated Zika virus NS1 conjugate offers advantages for accurate detection of Zika virus specific antibody in double antigen binding and Ig capture enzyme immunoassayseng
dc.typearticleeng
dc.rights.holderCopyright PUBLIC LIBRARY SCIENCEeng
dc.identifier.doi10.1371/journal.pone.0215708
dc.identifier.pmid31374094
dc.subject.wosMultidisciplinary Scienceseng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalTEDDER, Richard S.:Publ Hlth England, Blood Borne Virus Unit, Virus Reference Dept, London, England; NHS Blood & Transplant, Microbiol Serv, London, England; UCL, London, England; Imperial Coll London, Fac Med, Dept Infect Dis, London, England
hcfmusp.author.externalDICKS, Steve:Publ Hlth England, Blood Borne Virus Unit, Virus Reference Dept, London, England; NHS Blood & Transplant, Microbiol Serv, London, England
hcfmusp.author.externalIJAZ, Samreen:Publ Hlth England, Blood Borne Virus Unit, Virus Reference Dept, London, England
hcfmusp.author.externalLEVY, Flavia:Fiocruz MS, Flavivirus Reference Lab, IOC, Rio De Janeiro, Brazil
hcfmusp.author.externalMEDIALDEA-CARRERA, Raquel:Univ Liverpool, Natl Inst Hlth Res, Hlth Protect Res Unit Emerging & Zoonot Infect, Liverpool, Merseyside, England
hcfmusp.author.externalSEQUEIRA, Patricia Carvalho de:Fiocruz MS, Flavivirus Reference Lab, IOC, Rio De Janeiro, Brazil
hcfmusp.author.externalBROWN, David W. G.:Publ Hlth England, Blood Borne Virus Unit, Virus Reference Dept, London, England; Fiocruz MS, Flavivirus Reference Lab, IOC, Rio De Janeiro, Brazil
hcfmusp.author.externalLUMB, Ines Ushiro:Publ Hlth England, Blood Borne Virus Unit, Virus Reference Dept, London, England; NHS Blood & Transplant, Microbiol Serv, London, England
hcfmusp.description.articlenumbere0215708
hcfmusp.description.issue8
hcfmusp.description.volume14
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000484991500001
hcfmusp.origem.id2-s2.0-85070235831
hcfmusp.publisher.citySAN FRANCISCOeng
hcfmusp.publisher.countryUSAeng
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dc.description.indexMEDLINEeng
hcfmusp.citation.scopus2-
hcfmusp.scopus.lastupdate2022-05-05-
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Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICESP
Instituto do Câncer do Estado de São Paulo - HC/ICESP

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar


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