Performance of a Multiplex Nested Polymerase Chain Reaction in Detecting 7 Pathogens Containing DNA in Their Genomes Associated With Congenital Infections
Carregando...
Citações na Scopus
4
Tipo de produção
article
Data de publicação
2020
Título da Revista
ISSN da Revista
Título do Volume
Editora
COLL AMER PATHOLOGISTS
Autores
Citação
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, v.144, n.1, p.99-106, 2020
Resumo
Context.-Infections are the leading cause of perinatal and infant mortality in low-income and low-resource countries, which have a higher prevalence of infections. Definitive diagnosis of congenital and perinatal infections is largely dependent upon the results of laboratory tests. Objective.-To develop a multiplex nested polymerase chain reaction (PCR) technique for the simultaneous detection of 7 pathogens containing DNA in their genomes in suspected cases of congenital infection. Design.-Eligible participants were pregnant women with positive immunoglobulin M antibodies raised to one of the pathogens in the prenatal serologic screening, associated or not with fetal ultrasound abnormalities or positive fetal serology. Neonates whose mothers did not attend prenatal care were included when they presented with symptomatology and laboratory parameters suggestive of infection. The detection rate of the multiplex nested PCR was compared with maternal, fetal, and neonatal serology, as well as placental immunohistochemistry and noncommercial amplifications. Results.-Of 161 suspected cases, the multiplex nested PCR detected 60 (37.3%), whereas the tests available in hospital laboratories detected 13 of 60 (21.7%) of the cases detected by the multiplex nested PCR, demonstrating a 4.6 times higher detection rate for the multiplex nested PCR (Fisher exact test, P < .001). Positive amplifications were to Toxoplasma gondii (32 cases), cytomegalovirus (14 cases), parvovirus B19 (5 cases), and adenovirus (5 cases). In 4 cases, 2 pathogens were simultaneously detected. All types of biological matrices were suitable for amplification. Sequencing of multiplex nested PCR products confirmed the molecular findings. Conclusions.-The multiplex nested PCR significantly increased the number of diagnosed congenital infections. Given the scarcity of DNA recovered from amniotic fluid and some neonatal samples, this multiplex nested PCR allows the simultaneous detection of 7 pathogens associated with congenital infections in a reliable, faster, cost-effective, and more sensitive way.
Palavras-chave
Referências
- Aslanzadeh J, 2004, ANN CLIN LAB SCI, V34, P389
- Bailao Luiz Antonio, 2005, Ultrasound Q, V21, P295, DOI 10.1097/01.ruq.0000187025.61943.ff
- Baschat AA, 2003, AM J OBSTET GYNECOL, V189, P758, DOI 10.1067/S0002-9378(03)00720-8
- Buehler J, 2016, PLOS PATHOG, V12, DOI 10.1371/journal.ppat.1005655
- Burd EM, 2010, CLIN MICROBIOL REV, V23, P550, DOI 10.1128/CMR.00074-09
- BURG JL, 1989, J CLIN MICROBIOL, V27, P1787, DOI 10.1128/JCM.27.8.1787-1792.1989
- Camargo PR, 2011, INT J CARDIOL, V148, P204, DOI 10.1016/j.ijcard.2009.11.002
- Coyne CB, 2016, NAT REV MICROBIOL, V14, P707, DOI 10.1038/nrmicro.2016.125
- Crino JP, 2018, CLIN OBSTET GYNECOL, V61, P106, DOI 10.1097/GRF.0000000000000342
- Degani S, 2006, OBSTET GYNECOL SURV, V61, P329, DOI 10.1097/01.ogx.0000216518.85796.88
- DEMMLER GJ, 1988, J INFECT DIS, V158, P1177, DOI 10.1093/infdis/158.6.1177
- DONNER C, 1994, PRENATAL DIAG, V14, P1055, DOI 10.1002/pd.1970141108
- DROSE JA, 1991, RADIOLOGY, V178, P369, DOI 10.1148/radiology.178.2.1846239
- Goodrum F, 2016, ANNU REV VIROL, V3, P333, DOI 10.1146/annurev-virology-110615-042422
- Kennedy PGE, 2015, J GEN VIROL, V96, P1581, DOI 10.1099/vir.0.000128
- KIMURA H, 1991, J INFECT DIS, V164, P289, DOI 10.1093/infdis/164.2.289
- Kralik P, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.00108
- Lansky S, 2014, CAD SAUDE PUBLICA, V30, DOI 10.1590/0102-311X00133213
- Lawn JE, 2006, INT J EPIDEMIOL, V35, P706, DOI 10.1093/ije/dyl043
- Lazar M, 2016, EBIOMEDICINE, V3, P86, DOI 10.1016/j.ebiom.2015.11.050
- Madrid L, 2016, EXPERT REV ANTI-INFE, V14, P845, DOI 10.1080/14787210.2016.1215913
- Maldonado YA, 2017, PEDIATRICS, V139, DOI 10.1542/peds.2016-3860
- MANROE BL, 1979, J PEDIATR-US, V95, P89, DOI 10.1016/S0022-3476(79)80096-7
- Markus A, 2015, PLOS PATHOG, V11, DOI 10.1371/journal.ppat.1004885
- Mathews T J, 2007, Natl Vital Stat Rep, V55, P1
- McIver CJ, 2005, J CLIN MICROBIOL, V43, P5102, DOI 10.1128/JCM.43.10.5102-5110.2005
- Miller JL, 2009, J PERINAT MED, V37, P140, DOI 10.1515/JPM.2009.027
- da Silva AAM, 2014, CAD SAUDE PUBLICA, V30, DOI 10.1590/0102-311X00129613
- NAKAJIMAIIJIMA S, 1985, P NATL ACAD SCI USA, V82, P6133, DOI 10.1073/pnas.82.18.6133
- Neu N, 2015, CLIN PERINATOL, V42, P77, DOI 10.1016/j.clp.2014.11.001
- Nikolayevskyy V, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0149435
- Okay TS, 2009, CLINICS, V64, P171, DOI 10.1590/S1807-59322009000300004
- Ouedraogo AR, 2016, PAN AFR MED J, V24, DOI 10.11604/pamj.2016.24.223.9406
- Oyer CE, 2000, HUM PATHOL, V31, P1433, DOI 10.1053/hupa.2000.20408
- Pan American Health Organization, 2006, NEON HLTH CONT MAT N
- Phan AT, 2016, BIOCHEM BIOPH RES CO, V474, P71, DOI 10.1016/j.bbrc.2016.04.070
- Piedade D, 2016, VIRUSES-BASEL, V8, DOI 10.3390/v8060156
- Pomares C, 2016, J CLIN MICROBIOL, V54, P2448, DOI 10.1128/JCM.00487-16
- PUCHHAMMERSTOCKL E, 1991, J CLIN MICROBIOL, V29, P1513
- Puerari Diane, 2015, Rev. paul. pediatr., V33, P136, DOI 10.1016/j.rpped.2014.09.004
- Rabelo K, 2017, FRONT IMMUNOL, V8, DOI 10.3389/fimmu.2017.01704
- Ranucci-Weiss D, 1998, PRENATAL DIAG, V18, P182, DOI 10.1002/(SICI)1097-0223(199802)18:2<182::AID-PD225>3.0.CO;2-E
- Rawlinson WD, 2017, LANCET INFECT DIS, V17, pE177, DOI 10.1016/S1473-3099(17)30143-3
- Silasi M, 2015, AM J REPROD IMMUNOL, V73, P199, DOI 10.1111/aji.12355
- Smieja M, 2001, J CLIN MICROBIOL, V39, P1796, DOI 10.1128/JCM.39.5.1796-1801.2001
- Tabata T, 2016, AM J PATHOL, V186, P2970, DOI 10.1016/j.ajpath.2016.07.016
- Teixeira LE, 2013, REV SOC BRAS MED TRO, V46, P584, DOI 10.1590/0037-8682-0095-2013
- Thomas F, 2010, PHARMACOGENOMICS, V11, P1751, DOI [10.2217/pgs.10.170, 10.2217/PGS.10.170]
- TOROK TJ, 1992, CLIN INFECT DIS, V14, P149, DOI 10.1093/clinids/14.1.149
- UNICEF. United Nations Children's Fund, 2009, STAT WORLDS CHILDR 2
- Villard O, 2016, J CLIN MICROBIOL, V54, P3034, DOI 10.1128/JCM.01193-16
Coleções
Artigos e Materiais de Revistas Científicas - FM/MOG
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/48
Artigos e Materiais de Revistas Científicas - LIM/57
Carregar mais Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/48
Artigos e Materiais de Revistas Científicas - LIM/57