Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/35816
Title: | Insulin does not stimulate beta-alanine transport into human skeletal muscle |
Authors: | GONCALVES, Livia de Souza; KRATZ, Caroline; SANTOS, Livia; CARVALHO, Victor Henrique; SALES, Lucas Peixoto; NEMEZIO, Kleiner; LONGOBARDI, Igor; RIANI, Luiz Augusto; LIMA, Marcelo Miranda de Oliveira; SAITO, Tiemi; FERNANDES, Alan Lins; RODRIGUES, Joice; JAMES, Ruth Margaret; SALE, Craig; GUALANO, Bruno; GELONEZE, Bruno; MEDEIROS, Marisa Helena Gennari de; ARTIOLI, Guilherme Giannini |
Citation: | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.318, n.4, p.C777-C786, 2020 |
Abstract: | To test whether high circulating insulin concentrations influence the transport of beta-alanine into skeletal muscle at either saturating or subsaturating beta-alanine concentrations, we conducted two experiments whereby beta-alanine and insulin concentrations were controlled. In experiment 1, 12 men received supraphysiological amounts of beta-alanine intravenously (0.11 g.kg(-1).min(-1) for 150 min), with or without insulin infusion. beta-Alanine and carnosine were measured in muscle before and 30 min after infusion. Blood samples were taken throughout the infusion protocol for plasma insulin and beta-alanine analyses. beta-Alanine content in 24-h urine was assessed. In experiment 2, six men ingested typical doses of beta-alanine (10 mg/kg) before insulin infusion or no infusion. beta-Alanine was assessed in muscle before and 120 min following ingestion. In experiment 1, no differences between conditions were shown for plasma beta-alanine, muscle beta-alanine, muscle carnosine and urinary beta-alanine concentrations (all P > 0.05). In experiment 2, no differences between conditions were shown for plasma beta-alanine or muscle beta-alanine concentrations (all P > 0.05). Hyperinsulinemia did not increase beta-alanine uptake by skeletal muscle cells, neither when substrate concentrations exceed the V-max of beta-alanine transporter TauT nor when it was below saturation. These results suggest that increasing insulin concentration is not necessary to maximize beta-alanine transport into muscle following beta-alanine intake. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - LIM/17 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_GONCALVES_Insulin_does_not_stimulate_betaalanine_transport_into_human_2020.PDF Restricted Access | publishedVersion (English) | 1.08 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.