Lacaziosis: immunohistochemical evaluation of elements of the humoral response in cutaneous lesions
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Citações na Scopus
1
Tipo de produção
article
Data de publicação
2020
Título da Revista
ISSN da Revista
Título do Volume
Editora
INST MEDICINA TROPICAL SAO PAULO
Autores
ALEXANDRE, Ariane Fernandes
TAFURI, Wagner Luiz
BARBOZA, Tania Cristina
QUARESMA, Juarez Antonio Simoes
BRITO, Arival Cardoso de
NASCIMENTO, Gabriela Yasmin Francisca da Silva do
Citação
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.62, article ID e75, 5p, 2020
Resumo
Lacaziosis is a cutaneous mycosis caused by the fungus Lacazia loboi, described in different countries of Latin America and prevalent in the Amazon region. The ineffective immune response against the agent seems to be related to a Th2 pattern of cytokines. There are few reports exploring elements of the humoral response in these lesions. Our aim was to investigate some elements focusing on 13 cells, plasma cells and local expression of IgG and IgM antibodies. Forty skin biopsies of lower limbs were selected. The diagnosis of lacaziosis was based on direct mycological examination and histological analysis. The visualization of fungal cells was improved by using Gridley's staining. An immunohistochemical protocol was performed to detect the expression of B cells, plasma cells. IgG and IgM. A double staining was performed to explore the presence of yeasts in the cytoplasm of keratinocytes, using an anti-AE1 AE3 antibody over Gridley's staining. The inflammatory infiltrate consisted of macrophages, multinucleated giant cells, lymphocytes, and fibrosis. Fungal cells were frequent in the stratum corneum and in both, the dermis and, in 50% of the specimens, also in the epidermis. Cells expressing IgG were more abundant when compared to cells expressing IgM. B cells and the presence of IgG might indicate that the humoral response promotes a Th2 immune response resulting in an anti-inflammatory phenotype. Our results lead us to suggest a possible role of B cells and immunoglobulins in the mechanisms of lacaziosis pathogenesis.
Palavras-chave
Lacaziosis, Humoral response, Skin, Human lesions, Immunohistochemistry
Referências
- Alexandre AF, 2017, MED MYCOL, V55, P145, DOI 10.1093/mmy/myw059
- Azevedo MDS, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0145814
- Baruzzi R G, 1979, Rev Inst Med Trop Sao Paulo, V21, P303
- Bossart GD, 2019, FRONT IMMUNOL, V10, DOI 10.3389/fimmu.2019.01125
- Brito Arival Cardoso de, 2007, An. Bras. Dermatol., V82, P461, DOI 10.1590/S0365-05962007000500010
- Christensen Stephen M, 2017, PLoS Negl Trop Dis, V11, pe0005588, DOI 10.1371/journal.pntd.0005588
- Kanashiro-Galo L, 2016, MED MYCOL, V54, P23, DOI 10.1093/mmy/myv069
- Kima PE, 2000, J EXP MED, V191, P1063, DOI 10.1084/jem.191.6.1063
- Miles SA, 2005, J EXP MED, V201, P747, DOI 10.1084/jem.20041470
- Paniz-Mondolfi A, 2012, MYCOSES, V55, P298, DOI 10.1111/j.1439-0507.2012.02184.x
- PECHER SA, 1979, ALLERGOL IMMUNOPATH, V7, P439
- Silva D., 1996, Anais Brasileiros de Dermatologia, V71, P211
- Quaresma JAS, 2010, ACTA TROP, V114, P59, DOI 10.1016/j.actatropica.2009.12.005
- Stevens A, 1982, THEORY PRACTICE HIST
- Talhari S, 2012, CLIN DERMATOL, V30, P420, DOI 10.1016/j.clindermatol.2011.09.014
- Vilani-Moreno F. R., 1997, Anais Brasileiros de Dermatologia, V72, P433
- Vilani-Moreno Fatima Regina, 2005, Revista Iberoamericana de Micologia, V22, P44, DOI 10.1016/S1130-1406(05)70006-1
- Vilani-Moreno FR, 2011, MED MYCOL, V49, P643, DOI 10.3109/13693786.2010.547993
- Vilani-Moreno Fátima Regina, 2007, Rev. Inst. Med. trop. S. Paulo, V49, P97, DOI 10.1590/S0036-46652007000200006
- Wanasen N, 2008, INT J PARASITOL, V38, P417, DOI 10.1016/j.ijpara.2007.08.010
- Xavier MB, 2008, HUM PATHOL, V39, P269, DOI 10.1016/j.humpath.2007.06.016