Assessment of TSPAN Expression Profile and Their Role in the VSCC Prognosis
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Citações na Scopus
1
Tipo de produção
article
Data de publicação
2021
Título da Revista
ISSN da Revista
Título do Volume
Editora
MDPI
Autores
FERREIRA, Kelly Pedrozo
BAIOCCHI, Glauco
SOARES, Fernando Augusto
ROCHA, Rafael Malagoli
DOBROFF, Andrey Senos
Citação
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, n.9, article ID 5015, 22p, 2021
Resumo
The role and prognostic value of tetraspanins (TSPANs) in vulvar squamous cell carcinoma (VSCC) remain poorly understood. We sought to primarily determine, at both the molecular and tissue level, the expression profile of the TSPANs CD9, CD63, CD81, and CD82 in archived VSCC samples (n = 117) and further investigate their clinical relevance as prognostic markers. Our studies led us to identify CD63 as the most highly expressed TSPAN, at the gene and protein levels. Multicomparison studies also revealed that the expression of CD9 was associated with tumor size, whereas CD63 upregulation was associated with histological diagnosis and vascular invasion. Moreover, low expression of CD81 and CD82 was associated with worse prognosis. To determine the role of TSPANs in VSCC at the cellular level, we assessed the mRNA levels of CD63 and CD82 in established metastatic (SW962) and non-metastatic (SW954) VSCC human cell lines. CD82 was found to be downregulated in SW962 cells, thus supporting its metastasis suppressor role. However, CD63 was significantly upregulated in both cell lines. Silencing of CD63 by siRNA led to a significant decrease in proliferation of both SW954 and SW962. Furthermore, in SW962 particularly, CD63-siRNA also remarkably inhibited cell migration. Altogether, our data suggest that the differential expression of TSPANs represents an important feature for prognosis of VSCC patients and indicates that CD63 and CD82 are likely potential therapeutic targets in VSCC.
Palavras-chave
tetraspanins, VSCC, immunohistochemistry, HPV infection, prognosis, cell culture, siRNA
Referências
- Akagi EM, 2014, BMC CANCER, V14, DOI 10.1186/1471-2407-14-822
- An HJ, 2020, THORAC CANCER, V11, P1989, DOI 10.1111/1759-7714.13492
- Andersen CL, 2004, CANCER RES, V64, P5245, DOI 10.1158/0008-5472.CAN-04-0496
- Baek J, 2019, J BREAST CANCER, V22, P77, DOI 10.4048/jbc.2019.22.e9
- Baiocchi G, 2015, EJSO-EUR J SURG ONC, V41, P1653, DOI 10.1016/j.ejso.2015.09.024
- Baiocchi G, 2013, EJSO-EUR J SURG ONC, V39, P780, DOI 10.1016/j.ejso.2013.03.004
- Baiocchi G, 2013, EJSO-EUR J SURG ONC, V39, P339, DOI 10.1016/j.ejso.2013.02.012
- Bizzarri N, 2017, J OBSTET GYNAECOL, V37, P975, DOI 10.1080/01443615.2017.1318269
- Carloni V, 2013, ONCOGENE, V32, P2649, DOI 10.1038/onc.2012.268
- Carvalho K.C., 2015, J DIS MARKERS, V2, P1024
- Buim MEC, 2010, ORAL ONCOL, V46, P166, DOI 10.1016/j.oraloncology.2009.11.009
- Chai J, 2017, MOL MED REP, V15, P1527, DOI 10.3892/mmr.2017.6186
- Chai J, 2016, ONCOL REP, V36, P737, DOI 10.3892/or.2016.4893
- de Almeida BC, 2019, CELLS-BASEL, V8, DOI 10.3390/cells8111452
- Earnest JT, 2017, PLOS PATHOG, V13, DOI 10.1371/journal.ppat.1006546
- Fast LA, 2018, INT J MOL SCI, V19, DOI 10.3390/ijms19103007
- Garcia-Mayea Y, 2020, CANCERS, V12, DOI 10.3390/cancers12113269
- Guidone L.R.M., 2010, THESIS
- Hantak MP, 2019, J VIROL, V93, DOI 10.1128/JVI.01429-17
- Hemler ME, 2005, NAT REV MOL CELL BIO, V6, P801, DOI 10.1038/nrm1736
- Heo K, 2020, BIOMOLECULES, V10, DOI 10.3390/biom10030388
- Hiroshima K, 2019, EXP CELL RES, V384, DOI 10.1016/j.yexcr.2019.111622
- Hong IK, 2006, J BIOL CHEM, V281, P24279, DOI 10.1074/jbc.M601209200
- Hori H, 2004, J SURG RES, V117, P208, DOI 10.1016/j.jss.2004.01.014
- Huan J, 2015, INT J CLIN EXP PATHO, V8, P3054
- Huang CI, 1998, AM J PATHOL, V153, P973, DOI 10.1016/S0002-9440(10)65639-8
- Huang CL, 1997, INT J ONCOL, V11, P1045
- IKEYAMA S, 1993, J EXP MED, V177, P1231, DOI 10.1084/jem.177.5.1231
- Jee BK, 2006, BIOCHEM BIOPH RES CO, V342, P655, DOI 10.1016/j.bbrc.2006.01.153
- Jiang LJ, 2019, CLIN EXP MED, V19, P377, DOI 10.1007/s10238-019-00554-x
- Jones EL, 2016, J IMMUNOL, V196, P978, DOI 10.4049/jimmunol.1500357
- Kaprio T, 2020, HISTOL HISTOPATHOL, V35, P887, DOI 10.14670/HH-18-209
- Kramer F, 2013, GEBURTSH FRAUENHEILK, V73, P142, DOI 10.1055/s-0032-1328133
- Kudo K, 2019, FASEB J, V33, P3851, DOI 10.1096/fj.201800664RR
- Kumari S, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0050411
- Kussaibi Haitham, 2020, Asian Pac J Cancer Prev, V21, P2549, DOI 10.31557/APJCP.2020.21.9.2549
- Kusukawa J, 2001, J ORAL PATHOL MED, V30, P73, DOI 10.1034/j.1600-0714.2001.300202.x
- Kwon HJ, 2014, PATHOL RES PRACT, V210, P285, DOI 10.1016/j.prp.2014.01.004
- Lang T, 2020, CURR BIOL, V30, pR204, DOI 10.1016/j.cub.2020.01.007
- Lavorato-Rocha AM, 2015, METHODS, V77-78, P20, DOI 10.1016/j.ymeth.2014.12.017
- Lavorato-Rocha AM, 2016, MOL CANCER RES, V14, P720, DOI 10.1158/1541-7786.MCR-15-0366
- Lavorato-Rocha AM, 2013, TUMOR BIOL, V34, P3713, DOI 10.1007/s13277-013-0955-0
- Lavorato-Rocha AM, 2013, ANN SURG ONCOL, V20, P31, DOI 10.1245/s10434-012-2560-7
- Ma ZK, 2020, DIS MARKERS, V2020, DOI 10.1155/2020/8899924
- Maia BD, 2016, ONCOTARGET, V7, P49217, DOI 10.18632/oncotarget.10247
- Maia BD, 2014, HUM PATHOL, V45, P917, DOI 10.1016/j.humpath.2014.01.015
- Maia BD, 2013, GYNECOL OBSTET INVES, V75, P53, DOI 10.1159/000343758
- Maia BD, 2012, J TRANSL MED, V10, DOI 10.1186/1479-5876-10-150
- Malik FA, 2009, HISTOL HISTOPATHOL, V24, P519, DOI 10.14670/HH-24.519
- Mantovani G, 2020, J CANCER RES CLIN, V146, P1647, DOI 10.1007/s00432-020-03226-6
- Miyake M, 1996, CANCER RES, V56, P1244
- Mizoshiri N, 2019, CELL ONCOL, V42, P861, DOI 10.1007/s13402-019-00472-w
- Palisoul ML, 2017, GYNECOL ONCOL, V146, P305, DOI 10.1016/j.ygyno.2017.05.011
- Park CS, 2016, ONCOGENE, V35, P4540, DOI 10.1038/onc.2015.520
- Pols MS, 2009, EXP CELL RES, V315, P1584, DOI 10.1016/j.yexcr.2008.09.020
- Proctor L, 2020, INT J GYNECOL CANCER, V30, P100, DOI 10.1136/ijgc-2019-000793
- Protzel C, 2008, VIRCHOWS ARCH, V452, P369, DOI 10.1007/s00428-008-0590-0
- Quagliano A, 2020, BLOOD ADV, V4, P4393, DOI 10.1182/bloodadvances.2020001592
- Rakislova N, 2020, AM J SURG PATHOL, V44, P1506, DOI 10.1097/PAS.0000000000001540
- Rasmussen CL, 2018, INT J CANCER, V142, P1158, DOI 10.1002/ijc.31139
- Ridnour LA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0044081
- Robert JMH, 2021, DRUG DISCOV TODAY, V26, P56, DOI 10.1016/j.drudis.2020.10.022
- Rodrigues IS, 2013, BRIT J CANCER, V109, P184, DOI 10.1038/bjc.2013.273
- Rogers LJ, 2018, INT J GYNECOL OBSTET, V143, P4, DOI 10.1002/ijgo.12609
- Saito Y, 2006, CANCER RES, V66, P9557, DOI 10.1158/0008-5472.CAN-06-1131
- Sho M, 1998, INT J CANCER, V79, P509, DOI 10.1002/(SICI)1097-0215(19981023)79:5<509::AID-IJC11>3.0.CO;2-X
- Soyuer S, 2010, PATHOL RES PRACT, V206, P607, DOI 10.1016/j.prp.2010.04.004
- Spoden G, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003313
- Tavsan Z, 2020, CELL ADHES MIGR, V14, P57, DOI 10.1080/19336918.2020.1732761
- te Grootenhuis NC, 2018, GYNECOL ONCOL, V148, P622, DOI 10.1016/j.ygyno.2017.11.006
- Termini CM, 2017, FRONT CELL DEV BIOL, V5, DOI 10.3389/fcell.2017.00034
- Tessier-Cloutier B, 2021, MODERN PATHOL, V34, P508, DOI 10.1038/s41379-020-00651-3
- Thali M, 2011, BIOCHEM SOC T, V39, P529, DOI 10.1042/BST0390529
- Thali M, 2009, CURR TOP MICROBIOL, V339, P85, DOI 10.1007/978-3-642-02175-6_5
- Uchida S, 1999, BRIT J CANCER, V79, P1168, DOI 10.1038/sj.bjc.6690186
- van Spriel AB, 2010, MICROBES INFECT, V12, P106, DOI 10.1016/j.micinf.2009.11.001
- Vences-Catalan F, 2017, BIOCHEM SOC T, V45, P531, DOI 10.1042/BST20160478
- Woelber L, 2020, CURR OPIN OBSTET GYN, V32, P9, DOI 10.1097/GCO.0000000000000595
- Yang XH, 2001, CANCER RES, V61, P5284
- Yang YG, 2016, EXP HEMATOL, V44, P322, DOI 10.1016/j.exphem.2016.02.006
- Yuan N, 2020, CHIN J CHROMATOGR, V38, P36, DOI 10.3724/SP.J.1123.2019.06001
- Zeng TD, 2018, EUR REV MED PHARMACO, V22, P5928, DOI 10.26355/eurrev_201809_15922
- Zhang W, 2019, INT J ONCOL, V54, P1719, DOI 10.3892/ijo.2019.4742
- Zhang XA, 2003, CANCER RES, V63, P2665
- Zhang Y, 2020, EXP THER MED, V19, P755, DOI 10.3892/etm.2019.8244
- Zheng ZZ, 2017, WORLD J SURG ONCOL, V15, DOI 10.1186/s12957-017-1279-0