Exercise Training Restores Cardiac Protein Quality Control in Heart Failure

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art_DOURADO_Exercise_Training_Restores_Cardiac_Protein_Quality_Control_in_2012.PDF (682.79 KB)
Citações na Scopus
61
Tipo de produção
article
Data de publicação
2012
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
CAMPOS, Juliane C.
QUELICONI, Bruno B.
CUNHA, Telma F.
ZAMBELLI, Vanessa O.
BECHARA, Luiz R. G.
KOWALTOWSKI, Alicia J.
BRUM, Patricia C.
MOCHLY-ROSEN, Daria
FERREIRA, Julio C. B.
Citação
PLOS ONE, v.7, n.12, article ID e52764, 12p, 2012
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Exercise training is a well-known coadjuvant in heart failure treatment; however, the molecular mechanisms underlying its beneficial effects remain elusive. Despite the primary cause, heart failure is often preceded by two distinct phenomena: mitochondria dysfunction and cytosolic protein quality control disruption. The objective of the study was to determine the contribution of exercise training in regulating cardiac mitochondria metabolism and cytosolic protein quality control in a post-myocardial infarction-induced heart failure (MI-HF) animal model. Our data demonstrated that isolated cardiac mitochondria from MI-HF rats displayed decreased oxygen consumption, reduced maximum calcium uptake and elevated H2O2 release. These changes were accompanied by exacerbated cardiac oxidative stress and proteasomal insufficiency. Declined proteasomal activity contributes to cardiac protein quality control disruption in our MI-HF model. Using cultured neonatal cardiomyocytes, we showed that either antimycin A or H2O2 resulted in inactivation of proteasomal peptidase activity, accumulation of oxidized proteins and cell death, recapitulating our in vivo model. Of interest, eight weeks of exercise training improved cardiac function, peak oxygen uptake and exercise tolerance in MI-HF rats. Moreover, exercise training restored mitochondrial oxygen consumption, increased Ca2+-induced permeability transition and reduced H2O2 release in MI-HF rats. These changes were followed by reduced oxidative stress and better cardiac protein quality control. Taken together, our findings uncover the potential contribution of mitochondrial dysfunction and cytosolic protein quality control disruption to heart failure and highlight the positive effects of exercise training in re-establishing cardiac mitochondrial physiology and protein quality control, reinforcing the importance of this intervention as a nonpharmacological tool for heart failure therapy.
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URI
https://observatorio.fm.usp.br/handle/OPI/4521
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor