Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorBERTI, Denise A.-
dc.contributor.authorRUSSO, Lilian C.-
dc.contributor.authorCASTRO, Leandro M.-
dc.contributor.authorCRUZ, Lilian-
dc.contributor.authorGOZZO, Fabio C.-
dc.contributor.authorHEIMANN, Joel C.-
dc.contributor.authorLIMA, Fabio B.-
dc.contributor.authorOLIVEIRA, Ariclecio C.-
dc.contributor.authorANDREOTTI, Sandra-
dc.contributor.authorPRADA, Patricia O.-
dc.contributor.authorHEIMANN, Andrea S.-
dc.contributor.authorFERRO, Emer S.-
dc.identifier.citationPROTEOMICS, v.12, n.17, Special Issue, p.2668-2681, 2012-
dc.description.abstractIntracellular peptides generated by the proteasome and oligopeptidases have been suggested to function in signal transduction and to improve insulin resistance in mice fed a high-caloric diet. The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high-caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin-stimulated glucose uptake in 3T3-L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin-induced glucose uptake in 3T3-L1 adipocytes.-
dc.description.sponsorshipBrazilian National Research Council (CNPq) [559698/2009-7-Rede GENO-PROT]-
dc.description.sponsorshipUniversity of Sao Paulo (NAPNA-USP) [2011.1.9333.1.3]-
dc.description.sponsorshipSao Paulo State Research Foundation (FAPESP)-
dc.subjectAdipose tissue-
dc.subjectInsulin resistance-
dc.subjectPeptide-protein interaction-
dc.subject.othercoa-binding protein-
dc.subject.otherthimet oligopeptidase-
dc.subject.other3t3-l1 adipocytes-
dc.subject.otherinhibitor dbi-
dc.titleIdentification of intracellular peptides in rat adipose tissue: Insights into insulin resistance-
dc.rights.holderCopyright WILEY-BLACKWELL-
dc.subject.wosBiochemical Research Methods-
dc.subject.wosBiochemistry & Molecular Biology-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-, Denise A.:Univ Sao Paulo, Dept Cell Biol & Dev, BR-0550800 Sao Paulo, Brazil-, Lilian C.:Univ Sao Paulo, Dept Cell Biol & Dev, BR-0550800 Sao Paulo, Brazil-, Leandro M.:Univ Sao Paulo, Dept Cell Biol & Dev, BR-0550800 Sao Paulo, Brazil-, Lilian:Univ Sao Paulo, Dept Cell Biol & Dev, BR-0550800 Sao Paulo, Brazil-, Fabio C.:Univ Estadual Campinas, Inst Chem, Sao Paulo, Brazil-, Fabio B.:Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, Brazil-, Ariclecio C.:Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, Brazil-, Sandra:Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, Brazil-, Patricia O.:Univ Estadual Campinas, Inst Chem, Sao Paulo, Brazil-, Andrea S.:Proteimax Biotechnol LTDA, Sao Paulo, Brazil-, Emer S.:Univ Sao Paulo, Dept Cell Biol & Dev, BR-0550800 Sao Paulo, Brazil-
hcfmusp.description.issueSpecial Issue-
hcfmusp.relation.referenceAkopian TN, 1997, J BIOL CHEM, V272, P1791-
hcfmusp.relation.referenceArkin MR, 2009, CURR OPIN CHEM BIOL, V13, P284, DOI 10.1016/j.cbpa.2009.05.125-
hcfmusp.relation.referenceBAYNES C, 1991, J INTERN MED, V229, P267-
hcfmusp.relation.referenceBerti DA, 2009, J BIOL CHEM, V284, P14105, DOI 10.1074/jbc.M807916200-
hcfmusp.relation.referenceBHUIYAN J, 1995, METABOLISM, V44, P1185, DOI 10.1016/0026-0495(95)90013-6-
hcfmusp.relation.referenceBJORNTORP P, 1990, INFUSIONSTHERAPIE, V17, P24-
hcfmusp.relation.referenceBJORNTORP P, 1991, DIABETES CARE, V14, P1132, DOI 10.2337/diacare.14.12.1132-
hcfmusp.relation.referenceBlackard W G, 1979, Trans Am Clin Climatol Assoc, V90, P94-
hcfmusp.relation.referenceBLACKARD WG, 1978, ENDOCRINOLOGY, V103, P548-
hcfmusp.relation.referenceBluher M, 2004, J BIOL CHEM, V279, P31902, DOI 10.1074/jbc.M404570200-
hcfmusp.relation.referenceBOWEN L, 1991, METABOLISM, V40, P1025, DOI 10.1016/0026-0495(91)90124-F-
hcfmusp.relation.referenceBRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1006/abio.1976.9999-
hcfmusp.relation.referenceBurns-Hamuro LL, 2003, P NATL ACAD SCI USA, V100, P4072, DOI 10.1073/pnas.2628038100-
hcfmusp.relation.referenceBurton M, 2005, BIOCHEM J, V392, P299, DOI 10.1042/BJ20050664-
hcfmusp.relation.referenceCastro LM, 2010, AAPS J, V12, P608, DOI 10.1208/s12248-010-9224-y-
hcfmusp.relation.referenceChe FY, 2005, MOL CELL PROTEOMICS, V4, P1391, DOI 10.1074/mcp.T500010-MCP200-
hcfmusp.relation.referenceChe FY, 2005, J MASS SPECTROM, V40, P227, DOI 10.1002/jms.742-
hcfmusp.relation.referenceChe FY, 2007, J PROTEOME RES, V6, P4667, DOI 10.1021/pr060690r-
hcfmusp.relation.referenceChopra M, 2002, B WORLD HEALTH ORGAN, V80, P952-
hcfmusp.relation.referenceChurchill EN, 2009, TRENDS ENDOCRIN MET, V20, P25, DOI 10.1016/j.tem.2008.10.002-
hcfmusp.relation.referenceCOSTA E, 1991, LIFE SCI, V49, P325, DOI 10.1016/0024-3205(91)90440-M-
hcfmusp.relation.referenceCOTTRELL JS, 1994, PEPTIDE RES, V7, P115-
hcfmusp.relation.referenceCuatrecasas P, 1975, Recent Prog Horm Res, V31, P37-
hcfmusp.relation.referenceCunha FM, 2008, J BIOL CHEM, V283, P24448, DOI 10.1074/jbc.M801252200-
hcfmusp.relation.referenceElholm M, 2000, J LIPID RES, V41, P538-
hcfmusp.relation.referenceFaergeman NJ, 2004, BIOCHEM J, V380, P907, DOI 10.1042/BJ20031949-
hcfmusp.relation.referenceFERRERO P, 1986, P NATL ACAD SCI USA, V83, P827, DOI 10.1073/pnas.83.3.827-
hcfmusp.relation.referenceFERRERO P, 1986, BRAIN RES, V399, P136, DOI 10.1016/0006-8993(86)90607-4-
hcfmusp.relation.referenceFranch J, 2002, DIABETES, V51, P449-
hcfmusp.relation.referenceFricker LD, 2012, J PROTEOME RES, V11, P1981, DOI 10.1021/pr2012076-
hcfmusp.relation.referenceGaigg B, 2001, MOL BIOL CELL, V12, P1147-
hcfmusp.relation.referenceGARVEY WT, 1986, DIABETES, V35, P258, DOI 10.2337/diabetes.35.3.258-
hcfmusp.relation.referenceGAVIN JR, 1974, P NATL ACAD SCI USA, V71, P84, DOI 10.1073/pnas.71.1.84-
hcfmusp.relation.referenceGelman JS, 2011, J PROTEOME RES, V10, P1583, DOI 10.1021/pr100952f-
hcfmusp.relation.referenceGREEN H, 1974, CELL, V3, P127, DOI 10.1016/0092-8674(74)90116-0-
hcfmusp.relation.referenceGuilherme A, 2008, NAT REV MOL CELL BIO, V9, P367, DOI 10.1038/nrm2391-
hcfmusp.relation.referenceHan D, 2012, J PROTEOME RES, V11, P2206, DOI 10.1021/pr200990b-
hcfmusp.relation.referenceHaynes CM, 2010, MOL CELL, V37, P529, DOI 10.1016/j.molcel.2010.01.015-
hcfmusp.relation.referenceHeimann AS, 2005, PHYSIOL GENOMICS, V20, P173, DOI 10.1152/physiolgenomics.00145.2004-
hcfmusp.relation.referenceHORUK R, 1985, MOL CELL ENDOCRINOL, V42, P1, DOI 10.1016/0303-7207(85)90002-4-
hcfmusp.relation.referenceHOTAMISLIGIL GS, 1994, DIABETES, V43, P1271, DOI 10.2337/diabetes.43.11.1271-
hcfmusp.relation.referenceHotamisligil GS, 1999, J INTERN MED, V245, P621, DOI 10.1046/j.1365-2796.1999.00490.x-
hcfmusp.relation.referenceHOTAMISLIGIL GS, 1993, SCIENCE, V259, P87, DOI 10.1126/science.7678183-
hcfmusp.relation.referenceJordan BA, 2003, MOL PHARMACOL, V64, P1317, DOI 10.1124/mol.64.6.1317-
hcfmusp.relation.referenceKaneto H, 2004, NAT MED, V10, P1128, DOI 10.1038/nm1111-
hcfmusp.relation.referenceKheifets V, 2006, J BIOL CHEM, V281, P23218, DOI 10.1074/jbc.M602075200-
hcfmusp.relation.referenceKim JK, 2001, J CLIN INVEST, V108, P437, DOI 10.1172/JCI11559-
hcfmusp.relation.referenceKOLTERMAN OG, 1980, J CLIN INVEST, V65, P1272, DOI 10.1172/JCI109790-
hcfmusp.relation.referenceKondo T, 2010, SCIENCE, V329, P336, DOI 10.1126/science.1188158-
hcfmusp.relation.referenceKraegen EW, 2008, P NATL ACAD SCI USA, V105, P7627, DOI 10.1073/pnas.0803901105-
hcfmusp.relation.referenceLAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0-
hcfmusp.relation.referenceLink A. J., 2009, COLD SPRING HARB PRO, DOI [10.1101/pdbprot5110, DOI 10.1101/PDBPR0T5110]-
hcfmusp.relation.referenceMAEGAWA H, 1988, J BIOL CHEM, V263, P12629-
hcfmusp.relation.referenceMarker T, 2012, DIABETES, V61, P615, DOI 10.2337/db10-1574-
hcfmusp.relation.referenceMARSHALL S, 1980, J CLIN INVEST, V66, P763, DOI 10.1172/JCI109914-
hcfmusp.relation.referenceMatsuda K, 2007, NEUROSCIENCE, V150, P425, DOI 10.1016/j.neuroscience.2007.09.012-
hcfmusp.relation.referenceMiesel A, 2010, ANN NUTR METAB, V56, P127, DOI 10.1159/000278748-
hcfmusp.relation.referenceMinashima T, 2012, J BIOL CHEM, V287, P14803, DOI 10.1074/jbc.M111.297861-
hcfmusp.relation.referenceMorano C, 2008, ANAL CHEM, V80, P9298, DOI 10.1021/ac801654h-
hcfmusp.relation.referenceOLEFSKY JM, 1988, AM J MED, V85, P86, DOI 10.1016/0002-9343(88)90401-9-
hcfmusp.relation.referencePetrescu AD, 2003, J BIOL CHEM, V278, P51813, DOI 10.1074/jbc.M303858200-
hcfmusp.relation.referencePrada PO, 2005, ENDOCRINOLOGY, V146, P1576, DOI 10.1210/en.2004-0767-
hcfmusp.relation.referenceReits E, 2004, IMMUNITY, V20, P495, DOI 10.1016/S1074-7613(04)00074-3-
hcfmusp.relation.referenceRioli V, 2003, J BIOL CHEM, V278, P8547, DOI 10.1074/jbc.M212030200-
hcfmusp.relation.referenceRios C, 2006, BRIT J PHARMACOL, V148, P387, DOI 10.1038/sj.bjp.0706757-
hcfmusp.relation.referenceRoden M, 1996, J CLIN INVEST, V97, P2859, DOI 10.1172/JCI118742-
hcfmusp.relation.referenceROSENMUND C, 1994, NATURE, V368, P853, DOI 10.1038/368853a0-
hcfmusp.relation.referenceRubinstein M, 2009, BIOPOLYMERS, V91, P505, DOI 10.1002/bip.21164-
hcfmusp.relation.referenceRusso LC, 2012, PROTEOMICS, V12, P2641, DOI 10.1002/pmic.201200032-
hcfmusp.relation.referenceSaric T, 2004, J BIOL CHEM, V279, P46723, DOI 10.1074/jbc.M406537200-
hcfmusp.relation.referenceShi Jun, 2008, V456, P307, DOI 10.1007/978-1-59745-245-8_23-
hcfmusp.relation.referenceStebbins JL, 2008, P NATL ACAD SCI USA, V105, P16809, DOI 10.1073/pnas.0805677105-
hcfmusp.relation.referenceSTUDENT AK, 1980, J BIOL CHEM, V255, P4745-
hcfmusp.relation.referenceTaubes G, 2009, SCIENCE, V325, P256, DOI 10.1126/science.325_256-
hcfmusp.relation.referenceTHODE J, 1989, J LIPID RES, V30, P1299-
hcfmusp.relation.referenceUDENFRIE.S, 1972, SCIENCE, V178, P871, DOI 10.1126/science.178.4063.871-
hcfmusp.relation.referenceUsui I, 1999, HORM METAB RES, V31, P546, DOI 10.1055/s-2007-978793-
hcfmusp.relation.referenceVANDELOOSDRECHT AA, 1991, J IMMUNOL METHODS, V141, P15, DOI 10.1016/0022-1759(91)90205-T-
hcfmusp.relation.referenceVanEppsFung M, 1997, ENDOCRINOLOGY, V138, P4338, DOI 10.1210/en.138.10.4338-
hcfmusp.relation.referenceWIDEN E, 1992, DIABETOLOGIA, V35, P1140, DOI 10.1007/BF00401367-
hcfmusp.relation.referenceYang LN, 2010, EUR J PHARMACOL, V642, P47, DOI 10.1016/j.ejphar.2010.05.061-
hcfmusp.relation.referenceYATES JR, 1995, ANAL CHEM, V67, P1426, DOI 10.1021/ac00104a020-
hcfmusp.relation.referenceYuan MS, 2001, SCIENCE, V293, P1673, DOI 10.1126/science.1061620-
hcfmusp.relation.referenceZhang RJ, 2002, ANAL CHEM, V74, P3662, DOI 10.1021/ac025614w-
hcfmusp.relation.referenceZUNIGAGUARJARDO S, 1991, DIABETES RES CLIN PR, V14, P55, DOI 10.1016/0168-8227(91)90053-G-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - LIM/16
LIM/16 - Laboratório de Fisiopatologia Renal

Files in This Item:
File Description SizeFormat 
  Restricted Access
publishedVersion (English)857.7 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.