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Title: Immunodominant antibody responses directed to SARS-CoV-2 hotspot mutation sites and risk of immune escape
Authors: OLIVEIRA, Jamille RamosRUIZ, Cesar Manuel RemuzgoMACHADO, Rafael Rahal GuaragnaMAGAWA, Jhosiene YukariDAHER, Isabela PazottiURBANSKI, Alysson HenriqueSCHMITZ, Gabriela Justamante HaendelARCURI, Helen AndradeFERREIRA, Marcelo AlvesSASAHARA, Greyce LuriMEDEIROS, Giuliana Xavier deJR, Roberto Carlos Vieira SilvaDURIGON, Edison LuizBOSCARDIN, Silvia BeatrizROSA, Daniela SantoroSCHECHTMAN, DeborahNAKAYA, Helder. I. I.CUNHA-NETO, EdecioGADERMAIER, GabrieleKALIL, JorgeCOELHO, VeronicaSANTOS, Keity Souza
Citation: FRONTIERS IN IMMUNOLOGY, v.13, article ID 1010105, 15p, 2023
Abstract: IntroductionConsidering the likely need for the development of novel effective vaccines adapted to emerging relevant CoV-2 variants, the increasing knowledge of epitope recognition profile among convalescents and afterwards vaccinated with identification of immunodominant regions may provide important information. MethodsWe used an RBD peptide microarray to identify IgG and IgA binding regions in serum of 71 COVID-19 convalescents and 18 vaccinated individuals. ResultsWe found a set of immunodominant RBD antibody epitopes, each recognized by more than 30% of the tested cohort, that differ among the two different groups and are within conserved regions among betacoronavirus. Of those, only one peptide, P44 (S415-429), recognized by 68% of convalescents, presented IgG and IgA antibody reactivity that positively correlated with nAb titers, suggesting that this is a relevant RBD region and a potential target of IgG/IgA neutralizing activity. DiscussionThis peptide is localized within the area of contact with ACE-2 and harbors the mutation hotspot site K417 present in gamma (K417T), beta (K417N), and omicron (K417N) variants of concern. The epitope profile of vaccinated individuals differed from convalescents, with a more diverse repertoire of immunodominant peptides, recognized by more than 30% of the cohort. Noteworthy, immunodominant regions of recognition by vaccinated coincide with mutation sites at Omicron BA.1, an important variant emerging after massive vaccination. Together, our data show that immune pressure induced by dominant antibody responses may favor hotspot mutation sites and the selection of variants capable of evading humoral response.
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Artigos e Materiais de Revistas Científicas - COVID-19

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MPT
Departamento de Patologia - FM/MPT

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/19
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular

Artigos e Materiais de Revistas Científicas - LIM/59
LIM/59 - Laboratório de Biologia Celular

Artigos e Materiais de Revistas Científicas - LIM/60
LIM/60 - Laboratório de Imunologia Clínica e Alergia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

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