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https://observatorio.fm.usp.br/handle/OPI/52957
Title: | Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls |
Authors: | PEREIRA-MANFRO, Wania Ferraz; SILVA, Giselle Pereira da; COSTA, Priscilla Ramos; COSTA, Dayane Alves; FERREIRA, Bianca da Silva; BARRETO, Daniela Mena; FROTA, Ana Cristina Cisne; HOFER, Cristina Barroso; KALLAS, Esper Georges; MILAGRES, Lucimar Goncalves |
Citation: | REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.65, article ID e14, 8p, 2023 |
Abstract: | Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HLA-DR), CD38, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT), programmed death 1 (PD-1) and CD57 by flow cytometry, during approximately one year. Eleven HIV -infected (HI) and nine HIV-uninfected (HU) children/adolescents who received two doses or one dose of meningococcal C conjugate vaccine (MenC), respectively, were involved in this study. Blood samples were collected before the immunization (T0), 1-2 months after the first dose (T1), and 1-2 months after the second dose (T2), which was administered approximately one year after the first one. HI patients not receiving combined antiretroviral therapy (cART) showed a higher frequency of CD8 T cells TIGIT+, PD-1+ or CD57+, as well as a higher frequency of CD8 T cells co-expressing CD38/HLA-DR/TIGIT or CD38/ HLA-DR/PD-1 when compared to HI treated or HU individuals, at all times that they were assessed. CD8 T cells co-expressing CD38/DR/TIGIT were inversely correlated with the CD4/CD8 ratio but positively associated with viral load. The co-expression of CD38/DR/ TIGIT or CD38/DR/PD-1 on CD8 T cells was also inversely associated with the CD4 T cells expressing co-stimulatory molecules CD127/CD28. The results showed a higher expression of exhaustion/senescence markers on CD8 T cells of untreated HI children/adolescents and its correlations with viral load. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - LIM/60 Artigos e Materiais de Revistas Científicas - ODS/03 |
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File | Description | Size | Format | |
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art_PEREIRA-MANFRO_Expression_of_TIGIT_PD1_and_HLADRCD38_markers_on_.PDF | publishedVersion (English) | 1.3 MB | Adobe PDF | View/Open |
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