ANA MARIA RIBEIRO DE CASTRO DUARTE
Projetos de Pesquisa
Unidades Organizacionais
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
10 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 10
- Residual malaria of Atlantic Forest systems and the influence of anopheline fauna(2021) FERREIRA, Lucas Mendes; REZENDE, Helder Ricas; BUERY, Julyana Cerqueira; SILVA, Leonardo Santana da; FIGUEIREDO, Thaysa Carolina Cantanhede; FUX, Blima; DUARTE, Ana Maria Ribeiro de Castro; CERUTTI JUNIOR, CrispimIn Brazil, the Amazon region comprises 99.5% of the reported malaria cases. However, another hotspot of the disease is the Atlantic Forest regions, with the sporadic occurrence of autochthonous human cases. In such context, this study sought to investigate the role of anopheline mosquitoes (Diptera: Culicidae) in the residual malaria transmission in Atlantic Forest areas. Two rural areas in the Espirito Santo state were the surveyed sites. Mosquitoes were captured using Shannon trap and CDC light traps and identified into species based on morphological characters. Ecological indexes (Shannon-Wiener diversity, Simpson's dominance, Pielou equability, and Sorensen similarity) were the tools used in the anopheline fauna characterization and comparison along with the two explored areas. The assessment of the sampling adequacy in the studied areas was possible through the generation of a species accumulation curve. A correlation test verified the influence of climatic variables on the anopheline species abundance. A total of 1471 female anopheline mosquitoes were collected from May 2019 to April 2020, representing 13 species. The species richness was higher in Valsugana Velha (hypo-endemic) than in Alto Caparao (non-endemic). There was a significant variation in the species abundance between Valsugana Velha (n = 1438) and Alto Caparao (n = 33). The most abundant species was Anopheles (Kerteszia) cruzii complex Dyar and Knab, 1908 representing 87% of the total anophelines collected. These results suggest that the Plasmodium spp. circulation in Brazilian Atlantic Forest areas occurs mainly due to the high frequency of Anopheles (K.) cruzii complex, considered the principal vector of simian and human malaria in the region.
- Naturally Acquired Humoral Immunity against Malaria Parasites in Non-Human Primates from the Brazilian Amazon, Cerrado and Atlantic Forest(2020) MONTEIRO, Eliana Ferreira; FERNANDEZ-BECERRA, Carmen; ARAUJO, Maisa da Silva; MESSIAS, Mariluce Rezende; OZAKI, Luiz Shozo; DUARTE, Ana Maria Ribeiro de Castro; BUENO, Marina Galvao; CATAO-DIAS, Jose Luiz; CHAGAS, Carolina Romeiro Fernandes; MATHIAS, Bruno da Silva; SANTOS, Mayra Gomes dos; SANTOS, Stefanie Vanessa; HOLCMAN, Marcia Moreira; JR, Julio Cesar de Souza; KIRCHGATTER, KarinNon-human primates (NHPs) have been shown to be infected by parasites of the genusPlasmodium, the etiological agent of malaria in humans, creating potential risks of zoonotic transmission.Plasmodium brasilianum, a parasite species similar toP. malariaeof humans, have been described in NHPs from Central and South America, including Brazil. The merozoite surface protein 1 (MSP1), besides being a malaria vaccine candidate, is highly immunogenic. Due to such properties, we tested this protein for the diagnosis of parasite infection. We used recombinant proteins ofP. malariaeMSP1, as well as ofP. falciparumandP. vivax, for the detection of antibodies anti-MSP1 of these parasite species, in the sera of NHPs collected in different regions of Brazil. About 40% of the NHP sera were confirmed as reactive to the proteins of one or more parasite species. A relatively higher number of reactive sera was found in animals from the Atlantic Forest than those from the Amazon region, possibly reflecting the former more intense parasite circulation among NHPs due to their proximity to humans at a higher populational density. The presence ofPlasmodiumpositive NHPs in the surveyed areas, being therefore potential parasite reservoirs, needs to be considered in any malaria surveillance program.
- Atlantic Forest Malaria: A Review of More than 20 Years of Epidemiological Investigation(2021) BUERY, Julyana Cerqueira; ALENCAR, Filomena Euridice Carvalho de; DUARTE, Ana Maria Ribeiro de Castro; LOSS, Ana Carolina; VICENTE, Creuza Rachel; FERREIRA, Lucas Mendes; FUX, Blima; MEDEIROS, Marcia Melo; CRAVO, Pedro; AREZ, Ana Paula; CERUTTI JUNIOR, CrispimIn the south and southeast regions of Brazil, cases of malaria occur outside the endemic Amazon region near the Atlantic Forest in some coastal states, where Plasmodium vivax is the recognized parasite. Characteristics of cases and vectors, especially Anopheles (Kerteszia) cruzii, raise the hypothesis of a zoonosis with simians as reservoirs. The present review aims to report on investigations of the disease over a 23-year period. Two main sources have provided epidemiological data: the behavior of Anopheles vectors and the genetic and immunological aspects of Plasmodium spp. obtained from humans, Alouatta simians, and Anopheles spp. mosquitoes. Anopheles (K.) cruzii is the most captured species in the forest canopy and is the recognized vector. The similarity between P. vivax and Plasmodium simium and that between Plasmodium malariae and Plasmodium brasilianum shared between simian and human hosts and the involvement of the same vector in the transmission to both hosts suggest interspecies transfer of the parasites. Finally, recent evidence points to the presence of Plasmodium falciparum in a silent cycle, detected only by molecular methods in asymptomatic individuals and An. (K.) cruzii. In the context of malaria elimination, it is paramount to assemble data about transmission in such non-endemic low-incidence areas.
- Plasmodium simium: Population Genomics Reveals the Origin of a Reverse Zoonosis(2021) OLIVEIRA, Thais C. de; RODRIGUES, Priscila T.; EARLY, Angela M.; DUARTE, Ana Maria R. C.; BUERY, Julyana C.; BUENO, Marina G.; CATAO-DIAS, Jose L.; CERUTTI, Crispim; RONA, Luisa D. P.; NEAFSEY, Daniel E.; FERREIRA, Marcelo U.Background. The population history of Plasmodium simium, which causes malaria in sylvatic Neotropical monkeys and humans along the Atlantic Coast of Brazil, remains disputed. Genetically diverse P vivax populations from various sources, including the lineages that founded the species P simium, are thought to have arrived in the Americas in separate migratory waves. Methods. We use population genomic approaches to investigate the origin and evolution of P simium. Results. We find a minimal genome-level differentiation between P simium and present-day New World P vivax isolates, consistent with their common geographic origin and subsequent divergence on this continent. The meagre genetic diversity in P simium samples from humans and monkeys implies a recent transfer from humans to non-human primates - a unique example of malaria as a reverse zoonosis of public health significance. Likely genomic signatures of P simium adaptation to new hosts include the deletion of > 40% of a key erythrocyte invasion ligand, PvRBP2a, which may have favored more efficient simian host cell infection. Conclusions. New World P vivax lineages that switched from humans to platyrrhine monkeys founded the P simium population that infects nonhuman primates and feeds sustained human malaria transmission in the outskirts of major cities.
- Complexity of malaria transmission dynamics in the Brazilian Atlantic Forest(2021) DUARTE, Ana Maria Ribeiro de Castro; FERNANDES, Licia Natal; SILVA, Fabiana Santos; SICCHI, Igor Lucoves; MUCCI, Luis Filipe; CURADO, Izilda; FERNANDES, Aristides; MEDEIROS-SOUSA, Antonio Ralph; CERETTI-JUNIOR, Walter; MARRELLI, Mauro Toledo; EVANGELISTA, Eduardo; TEIXEIRA, Renildo; SUMMA, Juliana Laurito; NARDI, Marcello Schiavo; GARNICA, Margoth Ramos; LOSS, Ana Carolina; BUERY, Julyana Cerqueira; CERUTT, Crispim; PACHECO, M. Andreina; ESCALANTE, Ananias A.; SALLUM, Maria Anice Mureb; LAPORTA, Gabriel ZorelloPlasmodium malariae and Plasmodium vivax are protozoan parasites that can cause malaria in humans. They are genetically indistinguishable from, respectively, Plasmodium brasilianum and Plasmodium simium, i.e. parasites infecting New World non-human primates in South America. In the tropical rainforests of the Brazilian Atlantic coast, it has long been hypothesized that P. brasilianum and P. simium in platyrrhine primates originated from P. malariae and P. vivax in humans. A recent hypothesis proposed the inclusion of Plasmodium falciparum into the transmission dynamics between humans and non-human primates in the Brazilian Atlantic tropical rainforest. Herein, we assess the occurrence of human malaria in simians and sylvatic anophelines using field-collected samples in the Capivari-Monos Environmental Protection Area from 2015 to 2017. We first tested simian blood and anopheline samples. Two simian (Aloutta) blood samples (18%, n = 11) showed Plasmodium cytb DNA sequences, one for P. vivax and another for P. malariae. From a total of 9,416 anopheline females, we found 17 pools positive for Plasmodium species with a 18S qPCR assay. Only three showed P. cytb DNA sequence, one for P. vivax and the others for rodent malaria species (similar to Plasmodium chabaudi and Plasmodium berghei). Based on these results, we tested 25 rodent liver samples for the presence of Plasmodium and obtained P. falciparum cytb DNA sequence in a rodent (Oligoryzomys sp.) liver. The findings of this study indicate complex malaria transmission dynamics composed by parallel spillover-spillback of human malaria parasites, i.e. P. malariae, P. vivax, and P. falciparum, in the Brazilian Atlantic forest.
- Anopheles (Kerteszia) cruzii infected by Plasmodium in the Atlantic Forest indicates that the malaria transmission cycle is maintained even after howler monkeys' population decline(2022) FERREIRA, Lucas Mendes; REZENDE, Helder Ricas; FUX, Blima; ALENCAR, Filomena Euridice Carvalho De; LOSS, Ana Carolina; BUERY, Julyana Cerqueira; DUARTE, Ana Maria Ribeiro De Castro; CERUTTI JUNIOR, CrispimThe Atlantic Forests outside of the Amazon region in Brazil are low-frequency malaria hotspots. The disease behaves as a zoonosis maintained by nonhuman primates (NHPs), especially howler monkeys. Between 2016 and 2018, Brazil witnessed the largest yellow fever outbreak since 1980, resulting in massive declines in these NHP populations. However, reports of malaria cases continued in transmission areas. This scenario motivated this survey to determine the frequency of infection of the anophelines by Plasmodium species. Mosquitoes were captured using Shannon traps and CDC light traps and identified as to species based on morphological characters. The screening for malaria parasites targeted only Anopheles species belonging to the subgenus Kerteszia, the proven primary malaria vector. A TaqMan qPCR assay using ribosomal primers (18S rRNA gene) was performed in a Step One Plus Real-time PCR to detect Plasmodium species. Seven hundred sixty field-caught anophelines divided into 76 pools were examined. Out of 76 tested pools, seven (9.21%) were positive. Three pools were Plasmodium malariae-positive, and four were Plasmodium vivax-positive. The anopheline infection was expressed as the maximum infection rate (MIR), disclosing a value of 0.92%, indicative of a steady state. Such stability after the yellow fever outbreak suggests that other species of NHPs could support transmission.
conferenceObject RESEARCH OF PLASMODIUM DNA IN LIVERS OBTAINED FROM SIMIANS DEAD BY YELLOW FEVER(2019) FURIERI, C.; BUERY, J. C.; FUX, B.; PINA, B. P.; COLODETTI, A. F.; SILVA, F. S.; KAWANAMI, A.; MENDES, S. L.; DUARTE, A. M. R. C.; CERUTTI JUNIOR, C.- Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil(2023) RANGEL, Marina E. O.; DUARTE, Ana Maria R. C.; OLIVEIRA, Tatiane M. P.; MUCCI, Luis F.; LOSS, Ana Carolina; LOAIZA, Jose R.; LAPORTA, Gabriel Z.; SALLUM, Maria Anice M.Here, the main goal is to assess natural infections of Plasmodium spp. in anophelines in a forest reserve from the same region where we previously found a surprisingly high rate (5.2%) of plasmodia infections (n = 25) in Kerteszia mosquitoes (N = 480) on the slopes of Serra do Mar, Atlantic Forest, Brazil. The mosquito collection sampling was carried out at the Legado das aguas Forest Reserve using CDC light traps and Shannon traps at night (5-10 pm) in 3-day collections in November 2021 and March, April, May, and November 2022. The captured specimens were morphologically identified at the species level and had their genomic DNA extracted in pools of up to 10 mosquitoes/pool. Each pool was tested using 18S qPCR and cytb nested PCR plus sequencing. A total of 5301 mosquitoes, mostly belonging to the genus Kerteszia (99.7%), were sampled and sorted into 773 pools. Eight pools positive for Plasmodium spp. were identified: four for Plasmodium spp., one for P. vivax or P. simium, one for P. malariae or P. brasilianum, and two for the P. falciparum-like parasite. After Sanger sequencing, two results were further confirmed: P. vivax or P. simium and P. malariae or P. brasilianum. The minimum infection rate for Kerteszia mosquitoes was 0.15% (eight positive pools/5285 Kerteszia mosquitoes). The study reveals a lower-than-expected natural infection rate (expected = 5.2% vs. observed = 0.15%). This low rate relates to the absence of Alouatta monkeys as the main simian malaria reservoir in the studied region. Their absence was due to a significant population decline following the reemergence of yellow fever virus outbreaks in the Atlantic Forest from 2016 to 2019. However, this also indicates the existence of alternative reservoirs to infect Kerteszia mosquitoes. The found zoonotic species of Plasmodium, including the P. falciparum-like parasite, may represent a simian malaria risk and thus a challenge for malaria elimination in Brazil.
- Ongoing host-shift speciation in Plasmodium simium(2021) OLIVEIRA, Thais C. de; RODRIGUES, Priscila T.; DUARTE, Ana Maria R. C.; RONA, Luisa D. P.; FERREIRA, Marcelo U.Plasmodium simium, a malaria parasite that infects platyrrhine monkeys and humans in the New World, is nearly identical to Plasmodium vivax. Recent genomic comparative analyses of these sister species have identified elevated divergence in a gene that may underlie P. simium adaptation to non-human primates during its gradual speciation process.
- Simian malaria: a narrative review on emergence, epidemiology andthreatto global malaria elimination(2023) FORNACE, Kimberly M.; LAPORTA, Gabriel Zorello; VYTHILINGHAM, Indra; CHUA, Trock Hing; CHUA, rock Hing; AHMED, Kamruddin; JEYAPRAKASAM, Nantha K.; DUARTE, Ana Maria Ribeiro de Castro; AMIR, Amirah; PHANG, Wei Kit; DRAKELEY, Chris; SALLUM, Maria Anice M.; LAU, Yee LingSimian malaria from wild non-human primate populations is increasingly recognised as a public health threat and is now the main cause of human malaria in Malaysia and some regions of Brazil. In 2022, WHO changed malaria elimination certification guidelines to require non-negligible risks of simian malaria in people, leaving many countries with no pathway to elimination. We review the global distribution and drivers of simian malaria and identify priorities for diagnosis, treatment, surveillance, and control. Environmental change is driving closer interactions between humans and wildlife, with malaria parasites from non-human primates spilling over into human populations and human malaria parasites spilling back into wild non-human primate populations. These complex transmission cycles require new molecular and epidemiological approaches to track parasite spread. Current methods of malaria control are ineffective, with wildlife reservoirs and primarily outdoor-biting mosquito vectors urgently requiring the development of novel control strategies. Without these, simian malaria has the potential to undermine malaria elimination globally.