KATIA CRISTINA DANTAS
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- A single-centre, retrospective study of the incidence of invasive fungal infections during 85 years of autopsy service in Brazil(2021) DANTAS, Katia Cristina; MAUAD, Thais; ANDRE, Carmen D. Saldiva de; BIERRENBACH, Ana Luiza; SALDIVA, Paulo Hilario NascimentoAutopsy continues to play an essential role in monitoring opportunistic fungal infections. However, few studies have analysed the historical trends of fungal infections in autopsies. Here, we analyse available data on fungal infections obtained from autopsy reports during 85 years of autopsies performed by the largest autopsy service in Brazil. All invasive fungal infections presented in autopsy reports between 1930 and 2015 were included. Of the 158,404 autopsy reports analysed, 1096 involved invasive fungal infections. In general, paracoccidioidomycosis (24%) was the most frequent infection, followed by candidiasis (18%), pneumocystosis (11.7%), cryptococcosis (11%), aspergillosis (11%) and histoplasmosis (3.8%). Paracoccidioidomycosis decreased after the 1950s, whereas opportunistic fungal infections increased steadily after the 1980s during the peak of the AIDS pandemic. The lung was the most frequently affected organ (73%). Disseminated infection was present in 64.5% of cases. In 26% of the 513 cases for which clinical charts were available for review, the diagnosis of opportunistic fungal infections was performed only at autopsy. Our unique 85-year history of autopsies showed a transition from endemic to opportunistic fungal infections in SAo Paulo, Brazil, reflecting increased urbanization, the appearance of novel diseases, such as AIDS in the 1980s, and advances in medical care over time.
- Airborne microplastics and SARS-CoV-2 in total suspended particles in the area surrounding the largest medical centre in Latin America(2022) AMATO-LOURENCO, Luis Fernando; COSTA, Natalie de Souza Xavier; DANTAS, Katia Cristina; GALVA, Luciana dos Santos; MORALLES, Fernando Negri; LOMBARDI, Suzette Cleuza Ferreira Spina; JUNIOR, Alfredo Mendroni; LINDOSO, Jose Angelo Lauletta; ANDO, Romulo Augusto; LIMA, Felipe Gallego; CARVALHO-OLIVEIRA, Regiani; MAUAD, ThaisMicroplastics (MPs) have been reported in the outdoor/indoor air of urban centres, raising health concerns due to the potential for human exposure. Since aerosols are considered one of the routes of Coronavirus disease 2019 (COVID-19) transmission and may bind to the surface of airborne MPs, we hypothesize that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be associated with the levels of MPs in the air. Our goal was to quantify the SARS-CoV-2 RNA and MPs present in the total suspended particles (TSP) collected in the area surrounding the largest medical centre in Latin America and to elucidate a possible association among weather variables, MPs, and SARS-CoV-2 in the air. TSP were sampled from three outdoor locations in the areas surrounding a medical centre. MPs were quantified and measured under a fluorescence microscope, and their polymeric composition was characterized by Fourier transform infrared (FT-IR) microspectroscopy coupled with attenuated total reflectance (ATR). The viral load of SARS-CoV-2 was quantified by an in-house real-time PCR assay. A generalized linear model (GzLM) was employed to evaluate the effect of the SARS-CoV-2 quantification on MPs and weather variables. TSP samples tested positive for SARS-CoV-2 in 22 out of 38 samples at the three sites. Polyester was the most frequent polymer (80%) found in the samples. The total amount of MPs was positively associated with the quantification of SARS-CoV-2 envelope genes and negatively associated with weather variables (temperature and relative humidity). Our findings show that SARS-CoV-2 aerosols may bind to TSP, such as MPs, and facilitate virus entry into the human body.
- Four Years of Experience with the Sao Paulo University Medical School Community Garden(2018) DANTAS, Katia Cristina; ZEMBRUSKI, Paulo Sergio; KUBRUSLY, Marcia Saldanha; CARVALHO-OLIVEIRA, Regiani; MAUAD, ThaisThe School of Medicine, Sao Paulo University Community Garden (FMUSP Community Garden), formed in June 2013, occupies an area of 520 m(2). In the concreted area, vegetables and herbs are grown in large vessels (http://www.facebook.com/HortaDaFmusp). The garden runs on an agroecological basis using locally made compost (garden leaves and horse manure) and bio fertilizers provided by volunteers and the local restaurant (coffee powder). In the garden several herbs, medicinal plants, wild food plants and different types of seasonal vegetables are cultivated. The harvest is open for the entire community. Five medical students received financial support from the University to work 40 h per month to maintain the FMUSP Community Garden. Educational activities for the community include workshops (on medicinal herbs and wild food plants) and cooking events with students and volunteers including an elderly group, focused on healthy eating. In addition, a Ph.D. student conducted studies addressing the role of air pollution on urban gardens using the garden as an experimental site. In summary, the FMUSP Community Garden has provided sustainable, educational and research activities focused on sustainability and healthy eating in the medical campus, on a low budget, for the community. We believe this paper is important because it describes how this experience has benefited many health-related professionals and complements medical teaching. The FMUSP Community Garden has shown that agriculture in large urban centers is possible. The results were very promising, involving students, staff, patients and the surrounding community.
- Diffuse alveolar damage patterns reflect the immunological and molecular heterogeneity in fatal COVID-19(2022) ERJEFALT, Jonas S.; COSTA, Natalia de Souza Xavier; JONSSON, Jimmie; COZZOLINO, Olga; DANTAS, Katia Cristina; CLAUSSON, Carl-Magnus; SIDDHURAJ, Premkumar; LINDO, Caroline; ALYAMANI, Manar; LOMBARDI, Suzete Cleusa Ferreira Spina; MENDRONI JUNIOR, Alfredo; ANTONANGELO, Leila; FARIA, Caroline Silverio; DUARTE-NETO, Amaro Nunes; MONTEIRO, Renata Aparecida de Almeida; PINHO, Joao Renato Rebello; GOMES-GOUVEA, Michele Soares; PEREIRA, Roberta Verciano; MONTEIRO, Jhonatas Sirino; SETUBAL, Joao Carlos; OLIVEIRA, Ellen Pierre de; THEODORO FILHO, Jair; SANDEN, Caroline; ORENGO, Jamie M.; SLEEMAN, Matthew A.; SILVA, Luiz Fernando Ferraz da; SALDIVA, Paulo Hilario Nascimento; DOLHNIKOFF, Marisa; MAUAD, ThaisBackground Severe COVID-19 lung disease exhibits a high degree of spatial and temporal heterogeneity, with different histological features coexisting within a single individual. It is important to capture the disease complexity to support patient management and treatment strategies. We provide spatially decoded analyses on the immunopathology of diffuse alveolar damage (DAD) patterns and factors that modulate immune and structural changes in fatal COVID-19. Methods We spatially quantified the immune and structural cells in exudative, intermediate, and advanced DAD through multiplex immunohistochemistry in autopsy lung tissue of 18 COVID-19 patients. Cytokine profiling, viral, bacteria, and fungi detection, and transcriptome analyses were performed. Findings Spatial DAD progression was associated with expansion of immune cells, macrophages, CD8+ T cells, fibroblasts, and (lymph)angiogenesis. Viral load correlated positively with exudative DAD and negatively with dis-ease/hospital length. In all cases, enteric bacteria were isolated, and Candida parapsilosis in eight cases. Cytokines correlated mainly with macrophages and CD8+T cells. Pro-coagulation and acute repair were enriched pathways in exudative DAD whereas intermediate/advanced DAD had a molecular profile of elevated humoral and innate immune responses and extracellular matrix production. Interpretation Unraveling the spatial and molecular immunopathology of COVID-19 cases exposes the responses to SARS-CoV-2-induced exudative DAD and subsequent immune-modulatory and remodeling changes in proliferative/advanced DAD that occur side-by-side together with secondary infections in the lungs. These complex features have important implications for disease management and the development of novel treatments.
- Quantification of airborne SARS-CoV-2 genomic particles in different hospital settings(2021) AMATO-LOURENCO, Luis Fernando; COSTA, Natalia de Souza Xavier; DANTAS, Katia Cristina; LOMBARDI, Suzette Cleuza Ferreira Spina; MENDRONI JUNIOR, Alfredo; LINDOSO, Jose Angelo Lauletta; LIMA, Felipe Gallego; CARVALHO-OLIVEIRA, Regiani; MAUAD, ThaisWe quantified the presence of SARS-CoV-2 RNA in the air of different hospital settings and the autopsy room of the largest medical centre in Sao Paulo, Brazil. Real-time reverse-transcription PCR was used to determine the presence of the envelope protein of SARS-CoV-2 and the nucleocapsid protein genes. The E-gene was detected in 5 out of 6 samples at the ICU-COVID-19 ward and in 5 out of 7 samples at the ward-COVID-19. Similarly, in the non-dedicated facilities, the E-gene was detected in 5 out of 6 samples collected in the ICU and 4 out of 7 samples in the ward. In the necropsy room, 6 out of 7 samples were positive for the E-gene. When both wards were compared, the non-COVID ward presented a significantly higher concentration of the E-gene than in the COVID-19 ward (p = 0.003). There was no significant difference in E-gene concentration between the ICU-COVID-19 and the ICU (p = 0.548). Likewise, there was no significant difference among E-gene concentrations found in the autopsy room versus the ICUs and wards (dedicated or not) (p = 0.245). Our results show the widespread presence of aerosol contamination in different hospital units.