HELOISA JULIANA ZABEU ROSSI COSTA

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LIM/32 - Laboratório de Otorrinolaringologia, Hospital das Clínicas, Faculdade de Medicina

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  • article
    Assessment of a neurophysiological model of the mandibular branch of the facial nerve in rats by electromyography (vol 121, pg 179, 2012)
    (2012) SALOMONE, R.; COSTA, H. J. Z. R.; RODRIGUES, J. R. F.; SILVA, S. M. Reis e; OVANDO, P. C.; BENTO, R. F.
  • article 0 Citação(ões) na Scopus
    Polyethylene Glycol fusion associated with anti-oxidants: A new promise in the treatment of traumatic paralysis (vol 40, pg 1489, 2018)
    (2018) SALOMONE, Raquel; JACOMO, Alfredo Luiz; BENTO, Ricardo F.; NASCIMENTO, Silvia Bona do; LEZIROVITZ, Karina; HOJAIJ, Flavio Carneiro; COSTA, Heloisa J. Z. R.
  • article 41 Citação(ões) na Scopus
    Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate in vivo and Promote Facial Nerve Regeneration
    (2019) PEREIRA, Larissa Vilela; BENTO, Ricardo Ferreira; CRUZ, Dayane B.; MARCHI, Claudia; SALOMONE, Raquel; OITICICCA, Jeanne; COSTA, Marcio Paulino; HADDAD, Luciana A.; MINGRONI-NETTO, Regina Celia; COSTA, Heloisa Juliana Zabeu Rossi
    Post-traumatic lesions with transection of the facial nerve present limited functional outcome even after repair by gold-standard microsurgical techniques. Stem cell engraftment combined with surgical repair has been reported as a beneficial alternative. However, the best association between the source of stem cell and the nature of conduit, as well as the long-term postoperative cell viability are still matters of debate. We aimed to assess the functional and morphological effects of stem cells from human exfoliated deciduous teeth (SHED) in polyglycolic acid tube (PGAt) combined with autografting of rat facial nerve on repair after neurotmesis. The mandibular branch of rat facial nerve submitted to neurotmesis was repaired by autograft and PGAt filled with purified basement membrane matrix with or without SHED. Outcome variables were compound muscle action potential (CMAP) and axon morphometric. Animals from the SHED group had mean CMAP amplitudes and mean axonal diameters significantly higher than the control group (p < 0.001). Mean axonal densities were significantly higher in the control group (p = 0.004). The engrafted nerve segment resected 6 weeks after surgery presented cells of human origin that were positive for the Schwann cell marker (S100), indicating viability of transplanted SHED and a Schwann cell-like phenotype. We conclude that regeneration of the mandibular branch of the rat facial nerve was improved by SHED within PGAt. The stem cells integrated and remained viable in the neural tissue for 6 weeks since transplantation, and positive labeling for S100 Schwann-cell marker suggests cells initiated in vivo differentiation.
  • article 4 Citação(ões) na Scopus
    Combined polyglycolic acid tube and autografting versus autografting or polyglycolic acid tube alone. A comparative study of peripheral nerve regeneration in rats
    (2015) COSTA, Marcio Paulino; TEIXEIRA, Nuberto Hopfgartner; LONGO, Marco Vinicius Losso; GEMPERLI, Rolf; COSTA, Heloisa Juliana Zabeu Rossi
    PURPOSE: To compare sciatic nerve regeneration in rats using three different techniques of repair. METHODS: Fifteen isogonics rats were divided into three groups according to the method used to repair a 5-mm long defect created in the sciatic nerve: autogenous graft (Group A), polyglycolic acid tube (PGAt) (Group B), and of the association of PGAt with the graft (Group C). Histological analysis, regenerated myelinated axon number count and functional analysis were used to compare after six weeks. RESULTS: There was no difference in fiber diameter and degree of myelinization presented by Groups A, B and C. Group B presented the lowest number of regenerated axons. The groups did not display any significant functional difference after walking track analysis (p<0.05). CONCLUSION: No differences between the three groups in terms of functional recovery, although there were histological differences among them.
  • article 5 Citação(ões) na Scopus
    Mandibular Branch of the Facial Nerve in Wistar Rats: New Experimental Model to Assess Facial Nerve Regeneration
    (2014) BENTO, Ricardo Ferreira; SALOMONE, Raquel; NASCIMENTO, Silvia Bona do; FERREIRA, Ricardo Jose Rodriguez; SILVA, Ciro Ferreira da; COSTA, Heloisa Juliana Zabeu Rossi
    Introduction The ideal animal model for nerve regeneration studies is the object of controversy, because all models described by the literature have advantages and disadvantages. Objective To describe the histologic and functional patterns of the mandibular branch of the facial nerve of Wistar rats to create a new experimental model of facial nerve regeneration. Methods Forty-two male rats were submitted to a nerve conduction test of the mandibular branch to obtain the compound muscle action potential. Twelve of these rats had the mandibular branch surgically removed and submitted to histologic analysis (number, partial density, and axonal diameter) of the proximal and distal segments. Results There was no statistically significant difference in the functional and histologic variables studied. Conclusion These new histologic and functional standards of the mandibular branch of the facial nerve of rats establish an objective, easy, and greatly reproducible model for future facial nerve regeneration studies.
  • article 3 Citação(ões) na Scopus
    Quantitative histological analysis of the mandibular branch of the facial nerve in rats
    (2012) COSTA, Heloisa Juliana Zabeu Rossi; SALOMONE, Raquel; SILVA, Ciro Ferreira da; COSTA, Marcio Paulino; RAMOS, Beatriz Lucchetta; BENTO, Ricardo Ferreira
    PURPOSE: To establish a model to quantitative histological analysis of the mandibular branch of the facial nerve in rats. METHODS: Eleven Wistar rats had their right and left mandibular branches of the facial nerve surgically removed and were sacrificed afterwards. Quantitative histological analysis was performed with: a) partial number of axons; b) partial area of the transversal cut of the nerve (9000 mu m(2)); c) partial density. The averages of partial density were obtained. The statistical study was established by Wilcoxon test (p=0.05). RESULTS: In relation to density of axons, comparison between sides shows no statistically significant difference (p=0.248; p=0.533). Mean partial density of distal and proximal samples was, respectively, 0.18 +/- 0.02 and 0.19 +/- 0.02 axons/mu m(2). Comparison between proximal and distal samples shows no statistically significant difference (p=0.859; p=0.182). CONCLUSION: This study has successfully established a model to histological quantitative analysis of the mandibular branch of the facial nerve in rats.
  • article 12 Citação(ões) na Scopus
    Polyethylene glycol fusion associated with antioxidants: A new promise in the treatment of traumatic facial paralysis
    (2018) SALOMONE, Raquel; JACOMO, Alfredo Luiz; NASCIMENTO, Silvia Bona do; LEZIROVITZ, Karina; HOJAIJ, Flavio Carneiro; COSTA, Heloisa Juliana Zabeu Rossi; BENTO, Ricardo Ferreira
    Background: Recent studies in invertebrates have taught us that early cell membrane regeneration is determinant for axonal recovery and survival after trauma. Many authors obtained extraordinary results in neural regeneration using polyethylene glycol fusion protocols, which also involved microsutures and antioxidants. Methods: Sixty rats were evaluated with functional and histological protocol after facial nerve neurotmesis. Groups A and B had their stumps coapted with microsuture after 24 hours of neurotmesis and groups C and D after 72 hours. In addition to the microstructure, groups B and D used the polyethylene glycol-fusion protocol for the modulation of the Ca12. Results: At the sixth week, the latency of group D and duration of group B was lower than groups A and C (P 5.011). The axonal diameter of the groups that used polyethylene glycol-fusion was higher than those who did not use polyethylene glycol-fusion (P 001). Conclusion: Although not providing a functional improvement, polyethylene glycolfusion slowed down demyelination.
  • article 25 Citação(ões) na Scopus
    BONE MARROW STEM CELLS IN FACIAL NERVE REGENERATION FROM ISOLATED STUMPS
    (2013) SALOMONE, Raquel; BENTO, Ricardo F.; COSTA, Heloisa J. Z. R.; AZZI-NOGUEIRA, Deborah; OVANDO, Patricia C.; DA-SILVA, Cira F.; ZANATTA, Daniela B.; STRAUSS, Bryan E.; HADDAD, Luciana A.
    IntroductionSevere lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. Methods: We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann-like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated. Results: Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B. Conclusions: Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results.
  • article 46 Citação(ões) na Scopus
    Mesenchymal bone marrow stem cells within polyglycolic acid tube observed in vivo after six weeks enhance facial nerve regeneration
    (2013) COSTA, Heloisa Juliana Zabeu Rossi; BENTO, Ricardo Ferreira; SALOMONE, Raquel; AZZI-NOGUEIRA, Deborah; ZANATTA, Daniela B.; COSTA, Marcio Paulino; SILVA, Ciro Ferreira da; STRAUSS, Bryan E.; HADDAD, Luciana A.
    Autografting is the gold-standard method for facial nerve repair with tissue loss. Its association with high-quality scaffolds and cell implants has disclosed distinct experimental outcomes. The aim of this study was to evaluate the functional and histological effects of bone marrow stem cells (BMSC) combined with polyglycolic acid tube (PGAt) in autografted rat facial nerves. After neurotmesis of the mandibular branch of the rat facial nerve, surgical repair consisted of nerve autografting (groups A-E) contained in pGAT (groups B-E), filled with basement membrane matrix (groups C-E) with undifferentiated BMSC (group D) or Schwann-like cells that had differentiated from BMSC (group E). Axon morphometrics and an objective-compound muscle action potentials (CMAP) analysis were conducted. Immunofluorescence assays were carried out with Schwann cell marker S100 and anti-beta-galactosidase to label exogenous cells. Six weeks after surgery, animals from either cell-containing group had mean CMAP amplitudes significantly higher than control groups. Differently from other groups, facial nerves with Schwann-like cell implants had mean axonal densities within reference values. This same group had the highest mean axonal diameter in distal segments. We observed expression of the reporter gene lacZ in nerve cells in the graft and distally from it in groups D and E. Group-E cells had lacZ coexpressed with S100. In conclusion, regeneration of the facial nerve was improved by BMSC within PGAt in rats, yet Schwann-like cells were associated with superior effects. Accordingly, groups D and E had BMSC integrated in neural tissue with maintenance of former cell phenotype for six weeks.
  • article 13 Citação(ões) na Scopus
    Assessment of a Neurophysiological Model of the Mandibular Branch of the Facial Nerve in Rats by Electromyography
    (2012) SALOMONE, Raquel; COSTA, Heloisa Juliana Zabeu Rossi; RODRIGUES, Jose Ricardo Ferreira; SILVA, Samanta Marques Reis e; ORANDO, Patricia Camacho; BENTO, Ricardo Ferreira
    Objectives: Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography). Methods: Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CM APs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test. Results: There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA. Conclusions: This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.