Proficiency of DNA repair genes and microsatellite instability in operated colorectal cancer patients with clinical suspicion of lynch syndrome
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Citações na Scopus
8
Tipo de produção
article
Data de publicação
2015
Título da Revista
ISSN da Revista
Título do Volume
Editora
PIONEER BIOSCIENCE PUBL CO
Autores
CAVALCANTE, Juliana Magalhaes
CARRARO, Dirce
COUDRY, Renata de Almeida
DINIZ, Marcio Augusto
Citação
JOURNAL OF GASTROINTESTINAL ONCOLOGY, v.6, n.6, p.628-637, 2015
Resumo
Background: Lynch syndrome (LS) diagnosis is underestimated, and most of the patients remain undetected after colorectal resections. The study aims to assess the frequency of LS in patients undergoing surgical treatment for colorectal cancer (CRC). Methods: A total of 458 CRC patients were operated from January 2005 to December 2008. Positive CRC family history (FH) was present in 118 (25.8%) patients. Histologic sections were reviewed for microsatellite instability (MSI) criteria (Bethesda guidelines), immunohistochemical (IHC) analysis for MLH1, MSH2, MSH6, PMS2 proteins, through the avidin-biotin-peroxidase complex, MSI (BAT-25, BAT-26, NR-21, NR-24 and MONO-27) and BRAF somatic mutation. Results: Of the 118 patients with FH, 61 (51.69%) met at least one of the revised Bethesda criteria. IHC was abnormal in 8 (13.1%) and MSI in 12 patients (20%). BRAF was negative in all cases. MSI histopathological included: intratumoral lymphocytes (47.5%), expansive tumors (29.5%) mucinous component (27.8%) and Crohn's like reaction in (14.7%). There was an association between the revised Bethesda criteria with: sex, mucinous histology and Crohn's like reaction; MSI and IHC with PMS2 and MLH1. Revised Bethesda criteria 4 had 10.6 increased chances to display positive MSI. We have proposed a score to contribute as a practical tool in the diagnosis of LS. Conclusions: The frequence of LS in resected CRC patients was 2.6%. The criterion 4 Revised Bethesda was associated more strongly with the presence of MSI.
Palavras-chave
Hereditary nonpolyposis colorectal cancer (HNPCC), immunohistochemical (IHC), lynch syndrome (LS), microsatellite instability (MSI), B-raf
Referências
- Hampel H, 2005, NEW ENGL J MED, V352, P1851, DOI 10.1056/NEJMoa043146
- Gologan A, 2005, ARCH PATHOL LAB MED, V129, P1390
- Perez-Carbonell L, 2012, GUT, V61, P865, DOI 10.1136/gutjnl-2011-300041
- de la Chapelle A, 2004, NAT REV CANCER, V4, P769, DOI 10.1038/nrc1453
- Zhang LY, 2008, J MOL DIAGN, V10, P301, DOI 10.2353/jmoldx.2008.080062
- Wolf B, 2006, INT J CANCER, V118, P1465, DOI 10.1002/ijc.21524
- Hendriks YMC, 2006, CA-CANCER J CLIN, V56, P213
- Koehler-Santos P, 2011, WORLD J GASTROENTERO, V17, P766, DOI 10.3748/wjg.v17.i6.766
- Vasen HFA, 1999, GASTROENTEROLOGY, V116, P1453, DOI 10.1016/S0016-5085(99)70510-X
- RodriguezBigas MA, 1997, J NATL CANCER I, V89, P1758, DOI 10.1093/jnci/89.23.1758
- Kastrinos F, 2011, GASTROENTEROLOGY, V140, P73, DOI 10.1053/j.gastro.2010.08.021
- LYNCH HT, 1966, ARCH INTERN MED, V117, P206, DOI 10.1001/archinte.117.2.206
- VASEN HF, 1993, DIS COLON RECTUM, V36, P1, DOI 10.1007/BF02050292
- VASEN HFA, 1991, DIS COLON RECTUM, V34, P424, DOI 10.1007/BF02053699
- Goecke T, 2006, J CLIN ONCOL, V24, P4285, DOI 10.1200/JCO.2005.03.7333
- Truta B, 2008, FAM CANCER, V7, P267, DOI 10.1007/s10689-008-9186-8
- Jenkins MA, 2007, GASTROENTEROLOGY, V133, P48, DOI 10.1053/j.gastro.2007.04.044
- Serrano M, 2012, FAM CANCER, V11, P571, DOI 10.1007/s10689-012-9550-6
- Lynch HT, 2007, J NATL CANCER I, V99, P261, DOI 10.1093/jnci/djk077
- JASS JR, 1994, ANTICANCER RES, V14, P1631
- Kastrinos F, 2012, J CLIN ONCOL, V30, P1024, DOI 10.1200/JCO.2011.40.7171
- Pinol V, 2005, JAMA-J AM MED ASSOC, V293, P1986, DOI 10.1001/jama.293.16.1986
- Lynch HT, 1996, CANCER, V78, P1149, DOI 10.1002/(SICI)1097-0142(19960915)78:6<1149::AID-CNCR1>3.0.CO;2-5
- Lee HJ, 2010, AM J CLIN PATHOL, V133, P802, DOI 10.1309/AJCPO3F2ENKMDTUS
- Kakar S, 2004, MODERN PATHOL, V17, P696, DOI 10.1038/modpathol.3800093
- Umar A, 2004, J NATL CANCER I, V96, P261, DOI 10.1093/jnci/djh034
- Palomaki GE, 2009, GENET MED, V11, P42, DOI 10.1097/GIM.0b013e31818fa2db
- Urso E, 2008, INT J COLORECTAL DIS, V23, P801, DOI 10.1007/s00384-008-0484-2
- Ferreira S, 2009, ACTA MEDICA PORT, V22, P207
- Hampel H, 2008, J CLIN ONCOL, V26, P5783, DOI 10.1200/JCO.2008.17.5950
- Grover S, 2010, J NATL COMPR CANC NE, V8, P98
- Wright CL, 2003, AM J SURG PATHOL, V27, P1393, DOI 10.1097/00000478-200311000-00001
- Lynch HT, 2003, NEW ENGL J MED, V348, P919
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Artigos e Materiais de Revistas Científicas - FM/MPT
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