Calorie restriction increases cerebral mitochondrial respiratory capacity in a NO center dot-mediated mechanism: Impact on neuronal survival

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art_LAURINDO_Calorie_restriction_increases_cerebral_mitochondrial_respiratory_capacity_in_2012.PDF (409.28 KB)
Citações na Scopus
50
Tipo de produção
article
Data de publicação
2012
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE INC
Autores
CERQUEIRA, Fernanda M.
CUNHA, Fernanda M.
KOWALTOWSKI, Alicia J.
Citação
FREE RADICAL BIOLOGY AND MEDICINE, v.52, n.7, p.1236-1241, 2012
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Calorie restriction (CR) enhances animal life span and prevents age-related diseases, including neurological decline. Recent evidence suggests that a mechanism involved in CR-induced life-span extension is NO-stimulated mitochondrial biogenesis. We examine here the effects of CR on brain mitochondrial content. CR increased eNOS and nNOS and the content of mitochondria] proteins (cytochrome c oxidase, citrate synthase, and mitofusin) in the brain. Furthermore, we established an in vitro system to study the neurological effects of CR using serum extracted from animals on this diet. In cultured neurons, CR serum enhanced nNOS expression and increased levels of nitrite (a NO product). CR serum also enhanced the levels of cytochrome c oxidase and increased citrate synthase activity and respiratory rates in neurons. CR serum effects were inhibited by L-NAME and mimicked by the NO donor SNAP. Furthermore, both CR sera and SNAP were capable of improving neuronal survival. Overall, our results indicate that CR increases mitochondrial biogenesis in a NO-mediated manner, resulting in enhanced reserve respiratory capacity and improved survival in neurons.
Palavras-chave
Caloric restriction, nitric oxide synthase, mitochondrial biogenesis, aging
URI
https://observatorio.fm.usp.br/handle/OPI/1517
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/19