HLA-DQA1/B1 alleles as putative susceptibility markers in congenital toxoplasmosis

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Arquivos
art_SHIMOKAWA_HLADQA1B1_alleles_as_putative_susceptibility_markers_in_congenital_2016.PDF (999.67 KB)
Citações na Scopus
13
Tipo de produção
article
Data de publicação
2016
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
TAYLOR & FRANCIS INC
Autores
SHIMOKAWA, Paulo Tadashi
TARGA, Lilia Spaleta
Citação
VIRULENCE, v.7, n.4, p.456-464, 2016
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Host and parasite genotypes are among the factors associated with congenital toxoplasmosis pathogenesis. As HLA class II molecules play a key role in the immune system regulation, the aim of this study was to investigate whether HLA-DQA1/B1 alleles are associated with susceptibility or protection to congenital toxoplasmosis. One hundred and twenty-two fetuses with and 103 without toxoplasmosis were studied. The two study groups were comparable according to a number of socio-demographic and genetic variables. HLA alleles were typed by PCR-SSP. In the HLA-DQA1 region, the allele frequencies showed that *01:03 and *03:02 alleles could confer susceptibility ( OR= 3.06, p = 0.0002 and OR=9.60, p= 0.0001, respectively) as they were more frequent among infected fetuses. Regarding the HLA-DQB1 region, the *05: 04 allele could confer susceptibility ( OR = 6.95, p < 0.0001). Of the 122 infected fetuses, 10 presented susceptibility haplotypes contrasting with only one in the non-infected group. This difference was not statistically significant after correction for multiple comparison ( OR = 9.37, p=0.011). In the casuistic, there were two severely damaged fetuses with high parasite loads determined in amniotic fluid samples and HLA-DQA1 susceptibility alleles. In the present study, a discriminatory potential of HLA-DQA1/B1 alleles to identify susceptibility to congenital toxoplasmosis and the most severe cases has been shown.
Palavras-chave
Congenital toxoplasmosis, Genetic polymorphism, Genetic susceptibility, HLA alleles, polymerase chain reaction
URI
https://observatorio.fm.usp.br/handle/OPI/16267
Referências
  1. Bessieres MH, 2009, MEM I OSWALDO CRUZ, V104, P389, DOI 10.1590/S0074-02762009000200038
  2. Bidgoli S, 2011, J FR OPHTALMOL, V34, DOI 10.1016/j.jfo.2011.01.015
  3. Costa JM, 2000, J CLIN MICROBIOL, V38, P2929
  4. de Sorrentino AH, 2005, CLIN IMMUNOL, V115, P133, DOI 10.1016/j.clin.2005.01.003
  5. Dubey JP, 2012, PARASITOLOGY, V139, P1375, DOI 10.1017/S0031182012000765
  6. Gavinet MF, 1997, J CLIN MICROBIOL, V35, P1276
  7. Hill Dolores E., 2005, Animal Health Research Reviews, V6, P41, DOI 10.1079/AHR2005100
  8. HOWE DK, 1995, J INFECT DIS, V172, P1561
  9. Jaisson-Hot I, 2001, PRESSE MED, V30, P1001
  10. Klein J, 2000, NEW ENGL J MED, V343, P782
  11. Klein J, 2000, NEW ENGL J MED, V343, P702
  12. Kodjikian L, 2004, PEDIATR INFECT DIS J, V23, P272, DOI 10.1097/01.inf.0000115949.12206.69
  13. Kotb M, 2002, NAT MED, V8, P1398, DOI 10.1038/nm800
  14. Ladas ID, 1999, OPHTHALMOLOGICA, V213, P320, DOI 10.1159/000027446
  15. Lago EG, 2014, J PEDIAT-BRAZIL, V90, P363, DOI 10.1016/j.jped.2013.12.006
  16. Mack DG, 1999, INT J PARASITOL, V29, P1351, DOI 10.1016/S0020-7519(99)00152-6
  17. MEENKEN C, 1995, BRIT J OPHTHALMOL, V79, P494, DOI 10.1136/bjo.79.5.494
  18. Montoya JG, 2008, CLIN INFECT DIS, V47, P554, DOI 10.1086/590149
  19. Morelle C, 2012, J CLIN MICROBIOL, V50, P3977, DOI 10.1128/JCM.01959-12
  20. Mullighan CG, 1997, TISSUE ANTIGENS, V50, P688, DOI 10.1111/j.1399-0039.1997.tb02936.x
  21. Okay TS, 2009, CLINICS, V64, P171, DOI 10.1590/S1807-59322009000300004
  22. OLERUP O, 1992, TISSUE ANTIGENS, V39, P225, DOI 10.1111/j.1399-0039.1992.tb01940.x
  23. Pereira Ana Paula Esteves, 2013, BMC Res Notes, V6, P60, DOI 10.1186/1756-0500-6-60
  24. Prusa AR, 2015, CLIN MICROBIOL INFEC, V21
  25. Robert-Gangneux F, 2014, J INFECTION, V68, pS125, DOI 10.1016/j.jinf.2013.09.023
  26. Romand S, 2004, AM J OBSTET GYNECOL, V190, P797, DOI 10.1016/j.ajog.2003.09.039
  27. Sensini A, 2006, CLIN MICROBIOL INFEC, V12, P504, DOI 10.1111/j.1469-0691.2006.01444.x
  28. Silveira C, 2003, AM J OPHTHALMOL, V136, P370, DOI 10.1016/S0002-9394(03)00191-0
  29. Sterkers Y, 2012, J CLIN MICROBIOL, V50, P3944, DOI 10.1128/JCM.00918-12
  30. Tan TG, 2010, VACCINE, V28, P3977, DOI 10.1016/j.vaccine.2010.03.028
  31. Teixeira LE, 2013, REV SOC BRAS MED TRO, V46, P584, DOI 10.1590/0037-8682-0095-2013
  32. Thorsby E, 2009, TISSUE ANTIGENS, V74, P101, DOI 10.1111/j.1399-0039.2009.01291.x
  33. Trowsdale J, 2013, ANNU REV GENOM HUM G, V14, P301, DOI 10.1146/annurev-genom-091212-153455
  34. Valdes V, 2011, ARCH PEDIATRIE, V18, P761, DOI 10.1016/j.arcped.2011.04.011
  35. Villena I, 1998, SCAND J INFECT DIS, V30, P295
  36. Wallon M, 2001, EUR J PEDIATR, V160, P534, DOI 10.1007/s004310100805
  37. Wallon M, 2010, OBSTET GYNECOL, V115, P727, DOI 10.1097/AOG.0b013e3181d57b09

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPE
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/48