Fructose Acutely Stimulates NHE3 Activity in Kidney Proximal Tubule

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Arquivos
art_CRAJOINAS_Fructose_Acutely_Stimulates_NHE3_Activity_in_Kidney_Proximal_2012.pdf (1.68 MB)
Citações na Scopus
27
Tipo de produção
article
Data de publicação
2012
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
KARGER
Autores
QUEIROZ-LEITE, Gabriella D.
BEZERRA, Camila N. A.
REBOUCAS, Nancy Amaral
MALNIC, Gerhard
Citação
KIDNEY & BLOOD PRESSURE RESEARCH, v.36, n.1, p.320-334, 2012
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background/Aims: Fructose causes a sodium-sensitive hypertension and acutely reduces the urinary Na+ excretion, suggesting that it may regulate the activity of renal tubular sodium transporters. NHE3 is highly expressed in proximal tubule (PT), along with proteins that mediate fructose transport and metabolism. The present work was outlined to investigate whether fructose modulates proximal NHE3 activity and to elucidate the molecular mechanisms underlying this modulation. Methods/Results: Using in vivo stationary microperfusion, we observed that fructose stimulates NHE3 mediated JHCO(3)(-) reabsorption. The MAPK pathway is not involved in this activation, as demonstrated by using of MEK/MAPK inhibitors, whereas experiments using a PKA inhibitor suggest that PKA inhibition plays a role in this response. These results were confirmed in vitro by measuring the cell pH recovery rate after NH4Cl pulse in LLC-PK1, a pig PT cell line, which showed reduced cAMP levels and NHE3 phosphorylation at serine-552 (PKA consensus site) after fructose treatment. Conclusions: NHE3 activity is stimulated by fructose, which increases proximal tubule Na+ reabsorption. The molecular mechanisms involved in this process are mediated, at least in part, by downregulation of the PKA signaling pathway. Future studies are needed to address whether fructose-stimulated NHE3 activity may contribute to renal injury and hypertension.
Palavras-chave
Fructose, In situ microperfusion, cAMP, Sodium hydrogen exchangers, LLC-PK1 cells
URI
https://observatorio.fm.usp.br/handle/OPI/2630
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/13