Molecular diagnosis of Strongyloides stercoralis among transplant candidates
Carregando...
Citações na Scopus
8
Tipo de produção
article
Data de publicação
2018
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Citação
TRANSPLANT INFECTIOUS DISEASE, v.20, n.4, article ID e12909, 4p, 2018
Resumo
Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S.stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S.stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S.stercoralis infection among transplant candidates.
Palavras-chave
molecular diagnosis, Strongyloides stercoralis, transplant candidate
Referências
- Becker SL, 2015, ACTA TROP, V150, P210, DOI 10.1016/j.actatropica.2015.07.019
- Buonfrate D, 2015, EPIDEMIOL INFECT, V143, P452, DOI 10.1017/S0950268814001563
- Garcia LS, 2001, DIAGNOSTIC MED PARAS
- Keiser PB, 2004, CLIN MICROBIOL REV, V17, P208, DOI 10.1128/CMR.17.1.208-217.2004
- KOGA K, 1991, AM J TROP MED HYG, V45, P518, DOI 10.4269/ajtmh.1991.45.518
- Mejia R, 2012, CURR OPIN INFECT DIS, V25, P458, DOI 10.1097/QCO.0b013e3283551dbd
- Moghaddassani H, 2011, IRAN J PARASITOL, V6, P23
- Olsen A, 2009, T ROY SOC TROP MED H, V103, P967, DOI 10.1016/j.trstmh.2009.02.013
- Puthiyakunnon S, 2014, PLOS NEGLECT TROP D, V8, DOI 10.1371/journal.pntd.0003018
- SAIKI RK, 1985, SCIENCE, V230, P1350, DOI 10.1126/science.2999980
- Saugar JM, 2015, ACTA TROP, V142, P20, DOI 10.1016/j.actatropica.2014.10.020
- Schar F, 2013, ACTA TROP, V126, P89, DOI 10.1016/j.actatropica.2012.12.012
- Sitta RB, 2014, PARASITOLOGY, V141, P716, DOI 10.1017/S0031182013002035
- Stensvold CR, 2011, TRENDS PARASITOL, V27, P53, DOI 10.1016/j.pt.2010.11.005
- Stensvold CR, 2012, J CLIN MICROBIOL, V50, P540, DOI 10.1128/JCM.06012-11
- Uparanukraw P, 1999, AM J TROP MED HYG, V60, P967, DOI 10.4269/ajtmh.1999.60.967
- Verweij JJ, 2009, T ROY SOC TROP MED H, V103, P342, DOI 10.1016/j.trstmh.2008.12.001
- Wong SSY, 2014, EXP BIOL MED, V239, P1443, DOI 10.1177/1535370214523880
Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/06
Carregar mais Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/06