Human Synovial Mesenchymal Stem Cells Good Manufacturing Practices for Articular Cartilage Regeneration

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art_FERNANDES_Human_Synovial_Mesenchymal_Stem_Cells_Good_Manufacturing_Practices_2018.PDF (1.01 MB)
Citações na Scopus
33
Tipo de produção
article
Data de publicação
2018
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
MARY ANN LIEBERT, INC
Autores
KIMURA, Heitor Akio
PINHEIRO, Carla Cristina Gomes
SHIMOMURA, Kazunori
NAKAMURA, Norimasa
FERREIRA, Jose Ricardo
GOMOLL, Andreas H.
BUENO, Daniela Franco
Citação
TISSUE ENGINEERING PART C-METHODS, v.24, n.12, p.709-716, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite autologous chondrocyte implantation versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal stem cells (MSCs) may be isolated from various known tissues, including discarded fragments at arthroscopy such as synovial membrane. Choice of harvesting site is motivated by MSCs' abilities to modulate immunologic and inflammatory response through paracrine communication. Synovial MSCs have a greater proliferation and strong chondrogenic potential than bone and adipose MSCs and a less hypertrophic differentiation than bone MSCs. Good manufacturing practice (GMP) laboratory techniques for human clinical trials are still novel. To our knowledge, there are only two clinical trials in humans published since today. Purpose: Therefore, this work aimed to isolate and characterize synovial MSCs and evaluated their differentiation properties according to GMP standards. Materials and Methods: One-gram tissue sample from three patients of synovia was harvested at the beginning of arthroscopy surgery. MSCs were isolated, expanded, and characterized by flow cytometry. Results: It was possible to isolate and expand MSCs cultures from synovia, characterize MSCs by flow cytometry using proper monoclonal antibodies, and differentiate MSCs by coloring technique after chondrogenic, adipogenic, and osteogenic differentiations. Cartilage treatment may benefit from these tissue engineering protocols since arthroscopic procedures are routinely performed for different purposes in a previous stage and a favorable chondronegic differentiation cell lineage may be collected and stored in a less invasive way. Conclusion: Laboratory protocols established according to presented GMP were able to isolate and characterize MSCs obtained from synovia.
Palavras-chave
synovia, tissue engineering, mesenchymal stem cells, hyaline articular cartilage, chondrogenic differentiation, immune modulation
URI
https://observatorio.fm.usp.br/handle/OPI/29996
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MOT
Artigos e Materiais de Revistas Científicas - HC/IOT
Artigos e Materiais de Revistas Científicas - LIM/41