Airway smooth muscle proliferation and inflammation in asthma

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Arquivos
art_JAMES_Airway_smooth_muscle_proliferation_and_inflammation_in_asthma_2018.PDF (402.12 KB)
Citações na Scopus
29
Tipo de produção
article
Data de publicação
2018
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
AMER PHYSIOLOGICAL SOC
Autores
JAMES, Alan L.
NOBLE, Peter B.
DREW, Su-Ann
BAI, Tony R.
ABRAMSON, Michael J.
MCKAY, Karen O.
GREEN, Francis H. Y.
ELLIOT, John G.
Citação
JOURNAL OF APPLIED PHYSIOLOGY, v.125, n.4, p.1090-1096, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
In asthma, it is unclear if the airway smooth muscle cells proliferate more or are increased at the onset of asthma and remain stable. This study aimed to compare smooth muscle cell proliferation in individuals with and without asthma and correlate proliferation rates with cell size and number and with granulocytic airway inflammation. Postmortem airway sections were labeled with proliferating cell nuclear antigen (PCNA) and percent positive muscle cells calculated. On the same sections, smooth muscle cell size and number and the number of eosinophils and neutrophils were estimated and compared in cases of nonfatal (n = 15) and fatal (n = 15) asthma and control subjects (n = 15). The % PCNA(+) muscle cells was not significantly different in fatal (29.4 +/- 7.7%, mean +/- SD), nonfatal asthma (28.6 +/- 8.3%), or control subjects (24.6 = 6.7%) and not related to mean muscle cell size (r = 0.09), number (r = 0.36), thickness of the muscle layer (r = 0.05), or eosinophil numbers (r = 0.04) in the asthma cases. These data support the hypothesis that in asthma the increased thickness of the smooth muscle layer may be present before or at the onset of asthma and independent of concurrent granulocytic inflammation or exacerbation. NEW & NOTEWORTHY There is debate regarding the origins of the increased airway smooth muscle in asthma. It may be independent of inflammation or arise as a proliferative response to inflammation. The present study found no increase in the proportion of proliferating smooth muscle cells in asthma and no relation of proliferation to numbers of airway smooth muscle cells or inflammation. These results support a stable increase in smooth muscle in asthma that is independent of airway inflammation.
Palavras-chave
hyperplasia, hypertrophy, morphometry, proliferating cell nuclear antigen
URI
https://observatorio.fm.usp.br/handle/OPI/31819
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - ODS/03