LIM/53 - Laboratório de Micologia

URI Permanente desta comunidade

https://observatorio.fm.usp.br/handle/OPI/3806
O Laboratório de Micologia é ligado ao Departamento de Dermatologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP).

Linhas de pesquisa: imunopatogenia da paracoccidioidomicose humana; aplicação de modelos experimentais no estudo da imunidade celular e humoral em micoses sistêmicas; análise de polimorfismos e alterações gênicas de isolados fúngicos da coleção de culturas do LIM-53 e IMTSP/USP; padronização de técnicas moleculares no diagnóstico de infecções fúngicas sistêmicas e oportunistas.

Site oficial: http://limhc.fm.usp.br/portal/lim53-laboratorio-de-micologia/

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Diagnóstico laboratorial e radiológico
(2021) GIANNINI, Maria José Soares Mendes; NEGRO, Gilda Maria Barbaro Del; GONçALVES, Elmar Gonzaga
article 0 Citação(ões) na Scopus
Human interleukin-36γ plays a crucial role in cytokine induction during Sporothrix brasiliensis and S. schenckii infection in keratinocytes and PBMCs
(2024) KISCHKEL, Brenda; SANTOS, Jessica C. dos; LOPES-BEZERRA, Leila; TABORDA, Carlos P.; JOOSTEN, Leo A. B.
Cytokines of the interleukin (IL)-1 superfamily including the different IL-36 isoforms, have been reported as mediators of acute and chronic inflammation in human skin diseases, such as psoriasis. Here, we demonstrated for the first time that Sporothrix schenckii and S. brasiliensis, the fungi that cause subcutaneous infection sporotrichosis, can induce the expression of IL-36 alpha, IL-36 gamma and IL-36Ra in human keratinocytes and primary peripheral blood mononuclear cells (PBMCs). Specifically, IL-36 gamma was differentially expressed by keratinocytes stimulated with Sporothrix yeasts when compared to the commensal microorganism Staphylococcus epidermidis. The exposure of keratinocytes to 24 h or 7-days culture supernatant of PBMCs stimulated with Sporothrix induced higher IL-36 gamma production compared to direct stimulation of keratinocytes with the live fungus. We identified that IL-36 gamma mRNA expression in keratinocytes is increased in the presence of IL-17, TNF, IL-113 and IL-1 alpha and these cytokines may act synergistically to maintain IL-36 gamma production. Lastly, using a cohort of 164 healthy individuals, we showed that individuals carrying variants of the IL36G gene (rs11690399 and rs11683399) exhibit increased IL-36 gamma production as well as increased innate cytokine production after Sporothrix exposure. Importantly, stimulation of PBMCs with recombinant IL-36 gamma increased the production of IL-113 and IL-6, while IL-36Ra were able to decrease the concentration of these cytokines. Our findings contribute to the understanding of the pathogenesis of sporotrichosis and suggest that IL-36 gamma may be involved in maintaining the cytokine loop that leads to tissue destruction by exacerbating the immune response in sporotrichosis. Of high interest, we present the IL-36 signalling pathway as a potential new therapeutic target.
article 0 Citação(ões) na Scopus
Areata-Like Lupus as a Clinical Manifestation of Cutaneous Lupus Erythematosus
(2022) MORAIS, K. L.; SECCHIN, P.; ANZAI, A.; VERUSSA, M. J. M. C.; MUNCK, A.; FECHINE, C. O. C.; VALENTE, N. Y. S.; ROMITI, R.
Introduction: Lupus erythematosus (LE) is a chronic autoimmune disease that frequently causes hair loss and scalp lesions. Hair loss can be scarring and nonscarring, diffuse, or patchy. The nonscarring patchy alopecia is usually related to systemic LE (SLE) and may simulate alopecia areata (AA), reason why it is named areata-like lupus. Our case was diagnosed with areata-like lupus but did not meet criteria for SLE. Case Report: A 63-year-old woman presented with irregular nonscarring patchy alopecia in the temporal and frontoparietal scalp. Trichoscopy showed exclamation mark hairs, vellus hairs, and sparse yellow dots. Histology revealed epidermal vacuolar interface dermatitis, lymphohistiocytic infiltrate around the bulbs of anagen follicles, and eccrine glands. Direct immunofluorescence showed deposits of C3, IgA, and IgG in the basement membrane zone. Discussion: Patients with cutaneous LE can also manifest as nonscarring patchy alopecia that is clinically similar to AA, despite the absence of systemic manifestations. Areata-like lupus is secondary to the lupus autoimmune infiltrate that affects the skin including the hair follicles. Trichoscopy, histology, and direct immunofluorescence are important to differentiate this form of alopecia from AA, which is believed to have a higher incidence in lupus patients.
article 0 Citação(ões) na Scopus
Small fiber neuropathy and intractable scalp pruritus in dermatomyositis patients
(2023) CIRINO, P. V.; HORDINSKY, M.; MCADAMS, B.; ROMITI, R.
Background: Scalp pruritus is a common symptom in Dermatomyositis (DM) patients. There are indications that small nerve fibers neuropathy could be involved in this symptom, however the etiology of scalp pruritus is not fully understood. Objectives: To assess epidermal nerve fiber (ENF) density of dermatomyositis patients with scalp pruritus by biopsy by confocal microscopy and immunohistochemistry with subsequent imaging analysis. Methods: DM patients with severe scalp pruritus from the dermatology outpatient clinic were compared to healthy volunteers. Two 4-mm scalp skin biopsies were obtained above the right ear in the parietal region and below the occipital protuberance in the occipital region. Biopsy specimens were incubated with primary antibodies to protein gene product (PGP 9.5), calcitonin gene–related peptide (CGRP), substance P (SP) were used to visualize nerve fibers (ENF) and collagen IV was used to label the epidermal basement membrane. The number of ENFs per millimeter was counted and recorded as the mean of ± SD of counts in 16 images at two micrometer increments/sections, two from each of the samples. ENF densities were compared between groups and a multiple linear regression model was applied to associated factors with ENF density. Results: Fifteen DM patients with severe scalp pruritus and 12 healthy volunteers were included in the study. The mean number of ENF/mm in occipital region of DM group was 16.0 ± 13.9 while the control group in the same region was 99.8 ± 33.1. In parietal region the number of ENF/mm of DM group was 18.0 ± 20.7 while in control group was 50.4 ± 17.4 (p < 0.001). Conclusion: DM patients with pruritus could have some impairment of small nerve fiber density that could explain their recalcitrant scalp pruritus.
article 0 Citação(ões) na Scopus
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