Long-term endogenous acetylcholine deficiency potentiates pulmonary inflammation in a murine model of elastase-induced emphysema

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art_BANZATO_Longterm_endogenous_acetylcholine_deficiency_potentiates_pulmonary_inflammation_in_2021.PDF (3.64 MB)
Citações na Scopus
1
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
NATURE PORTFOLIO
Autores
PINHEIRO, Nathalia M.
SANTANA, Fernanda R.
CAPERUTO, Luciana C.
CAMARA, Niels O.
PRADO, Marco Antonio M.
Citação
SCIENTIFIC REPORTS, v.11, n.1, article ID 15918, 13p, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Acetylcholine (ACh), the neurotransmitter of the cholinergic system, regulates inflammation in several diseases including pulmonary diseases. ACh is also involved in a non-neuronal mechanism that modulates the innate immune response. Because inflammation and release of pro-inflammatory cytokines are involved in pulmonary emphysema, we hypothesized that vesicular acetylcholine transport protein (VAChT) deficiency, which leads to reduction in ACh release, can modulate lung inflammation in an experimental model of emphysema. Mice with genetical reduced expression of VAChT (VAChT KDHOM 70%) and wild-type mice (WT) received nasal instillation of 50 uL of porcine pancreatic elastase (PPE) or saline on day 0. Twenty-eight days after, animals were evaluated. Elastase instilled VAChT KDHOM mice presented an increase in macrophages, lymphocytes, and neutrophils in bronchoalveolar lavage fluid and MAC2-positive macrophages in lung tissue and peribronchovascular area that was comparable to that observed in WT mice. Conversely, elastase instilled VAChT KDHOM mice showed significantly larger number of NF-kappa B-positive cells and isoprostane staining in the peribronchovascular area when compared to elastase-instilled WT-mice. Moreover, elastase-instilled VAChT-deficient mice showed increased MCP-1 levels in the lungs. Other cytokines, extracellular matrix remodeling, alveolar enlargement, and lung function were not worse in elastase-instilled VAChT deficiency than in elastase-instilled WT-controls. These data suggest that decreased VAChT expression may contribute to the pathogenesis of emphysema, at least in part, through NF-kappa B activation, MCP-1, and oxidative stress pathways. This study highlights novel pathways involved in lung inflammation that may contribute to the development of chronic obstrutive lung disease (COPD) in cholinergic deficient individuals such as Alzheimer's disease patients.
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URI
https://observatorio.fm.usp.br/handle/OPI/42043
Referências
  1. Agusti A, 2012, AM J RESP CRIT CARE, V185, P1171, DOI 10.1164/rccm.201203-0505UP
  2. Anciaes AM, 2011, CLINICS, V66, P1797, DOI 10.1590/S1807-59322011001000020
  3. Angeli P, 2008, AM J PHYSIOL-LUNG C, V294, pL1197, DOI 10.1152/ajplung.00199.2007
  4. Baggio C, 2020, J MED CHEM, V63, P12911, DOI 10.1021/acs.jmedchem.0c01285
  5. Barnes PJ, 2006, LAB INVEST, V86, P867, DOI 10.1038/labinvest.3700456
  6. Barnes PJ, 1997, NEW ENGL J MED, V336, P1066, DOI 10.1056/NEJM199704103361506
  7. Belmonte Kristen E, 2005, Proc Am Thorac Soc, V2, P297, DOI 10.1513/pats.200504-043SR
  8. Borovikova LV, 2000, NATURE, V405, P458, DOI 10.1038/35013070
  9. Bracke Ken R., 2007, Inflammation & Allergy Drug Targets, V6, P75
  10. Brasier AR, 2018, EXPERT REV RESP MED, V12, P931, DOI 10.1080/17476348.2018.1526677
  11. Budulac SE, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0033386
  12. Ceylan E, 2006, RESP MED, V100, P1270, DOI 10.1016/j.rmed.2005.10.011
  13. Chung KF, 2008, EUR RESPIR J, V31, P1334, DOI 10.1183/09031936.00018908
  14. Chung KF, 2001, EUR RESPIR J, V18, p50S, DOI 10.1183/09031936.01.00229701
  15. Cruz FF, 2012, RESP PHYSIOL NEUROBI, V182, P26, DOI 10.1016/j.resp.2012.01.002
  16. de Boer WI, 2000, J PATHOL, V190, P619, DOI 10.1002/(SICI)1096-9896(200004)190:5<619::AID-PATH555>3.0.CO;2-6
  17. de Castro BM, 2009, MOL CELL BIOL, V29, P5238, DOI 10.1128/MCB.00245-09
  18. Di Stefano A, 2002, EUR RESPIR J, V20, P556, DOI 10.1183/09031936.02.00272002
  19. Douaoui S, 2020, IMMUNOBIOLOGY, V225, DOI 10.1016/j.imbio.2020.151950
  20. Favier M, 2020, J CLIN INVEST, V130, P6616, DOI 10.1172/JCI138532
  21. Fuke S, 2004, AM J RESP CELL MOL, V31, P405, DOI 10.1165/rcmb.2004-0131OC
  22. Gallowitsch-Puerta M, 2005, ANN NY ACAD SCI, V1062, P209, DOI 10.1196/annals.1358.024
  23. Gallowitsch-Puerta M, 2007, LIFE SCI, V80, P2325, DOI 10.1016/j.lfs.2007.01.002
  24. Geraghty P, 2013, FRONT PHYSIOL, V4, DOI 10.3389/fphys.2013.00267
  25. Gessner C, 2005, RESP MED, V99, P1229, DOI 10.1016/j.rmed.2005.02.041
  26. GOLD, 2020, G I F C O L D GLOB I
  27. Gosens R, 2006, RESP RES, V7, DOI 10.1186/1465-9921-7-73
  28. Guzman MS, 2011, PLOS BIOL, V9, DOI 10.1371/journal.pbio.1001194
  29. Gwilt CR, 2007, PHARMACOL THERAPEUT, V115, P208, DOI 10.1016/j.pharmthera.2007.05.007
  30. Hacievliyagil SS, 2013, NIGER J CLIN PRACT, V16, P76, DOI 10.4103/1119-3077.106771
  31. Hacievliyagil SS, 2006, RESP MED, V100, P846, DOI 10.1016/j.rmed.2005.08.022
  32. Hantos Z, 2008, J APPL PHYSIOL, V105, P1864, DOI 10.1152/japplphysiol.90924.2008
  33. Hogg JC, 2004, NEW ENGL J MED, V350, P2645, DOI 10.1056/NEJMoa032158
  34. Iho S, 2003, J LEUKOCYTE BIOL, V74, P942, DOI 10.1189/jlb.1202626
  35. Ito JT, 2019, CELLS-BASEL, V8, DOI 10.3390/cells8040342
  36. Kanazawa H, 2005, CHEST, V128, P3191, DOI 10.1378/chest.128.5.3191
  37. Kolisnyk B, 2013, J NEUROSCI, V33, P14908, DOI 10.1523/JNEUROSCI.1933-13.2013
  38. Kolisnyk B, 2013, J NEUROSCI, V33, P10427, DOI 10.1523/JNEUROSCI.0395-13.2013
  39. Kononov S, 2001, AM J RESP CRIT CARE, V164, P1920, DOI 10.1164/ajrccm.164.10.2101083
  40. Kummer W, 2008, HISTOCHEM CELL BIOL, V130, P219, DOI 10.1007/s00418-008-0455-2
  41. Lancas T, 2006, J APPL PHYSIOL, V100, P1610, DOI 10.1152/japplphysiol.00828.2005
  42. Lara A, 2010, MOL CELL BIOL, V30, P1746, DOI 10.1128/MCB.00996-09
  43. Lima RD, 2010, J NEUROCHEM, V113, P943, DOI 10.1111/j.1471-4159.2010.06657.x
  44. Lopes FDTQS, 2009, ENVIRON RES, V109, P544, DOI 10.1016/j.envres.2009.03.002
  45. MacNee William, 2005, Proc Am Thorac Soc, V2, P258, DOI 10.1513/pats.200504-045SR
  46. MacNee William, 2009, Proc Am Thorac Soc, V6, P527, DOI 10.1513/pats.200905-027DS
  47. Manoury Boris, 2006, J Inflamm (Lond), V3, P2, DOI 10.1186/1476-9255-3-2
  48. MARGRAF LR, 1991, AM REV RESPIR DIS, V143, P391
  49. Martins-Olivera BT, 2016, MEDIAT INFLAMM, V2016, DOI 10.1155/2016/5346574
  50. Montuschi P, 1999, EUR J PHARMACOL, V365, P59, DOI 10.1016/S0014-2999(98)00859-0
  51. Monzon ME, 2011, AM J PHYSIOL-LUNG C, V300, pL204, DOI 10.1152/ajplung.00292.2010
  52. Neumann S, 2007, LIFE SCI, V80, P2361, DOI 10.1016/j.lfs.2007.01.010
  53. Oenema TA, 2010, RESP RES, V11, DOI 10.1186/1465-9921-11-130
  54. Pavlov VA, 2006, BIOCHEM SOC T, V34, P1037, DOI 10.1042/BST0341037
  55. Pera T, 2011, EUR RESPIR J, V38, P789, DOI 10.1183/09031936.00146610
  56. Pereira MR, 2016, J CELL PHYSIOL, V231, P1862, DOI 10.1002/jcp.25307
  57. Pinheiro NM, 2020, EUR J PHARMACOL, V882, DOI 10.1016/j.ejphar.2020.173239
  58. Pinheiro NM, 2017, FASEB J, V31, P320, DOI 10.1096/fj.201600431R
  59. Pinheiro NM, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0120441
  60. Prado MAM, 2002, NEUROCHEM INT, V41, P291, DOI 10.1016/S0197-0186(02)00044-X
  61. Prado VF, 2006, NEURON, V51, P601, DOI 10.1016/j.neuron.2006.08.005
  62. Rosas-Ballina M, 2009, J INTERN MED, V265, P663, DOI 10.1111/j.1365-2796.2009.02098.x
  63. Rosas-Ballina M, 2011, SCIENCE, V334, P98, DOI 10.1126/science.1209985
  64. Roy A, 2013, FASEB J, V27, P5072, DOI 10.1096/fj.13-238279
  65. Sales DS, 2017, COPD, V14, P533, DOI 10.1080/15412555.2017.1346069
  66. Santana FPR, 2019, ECOTOX ENVIRON SAFE, V167, P494, DOI 10.1016/j.ecoenv.2018.10.005
  67. Schuliga M, 2015, BIOMOLECULES, V5, P1266, DOI 10.3390/biom5031266
  68. Shapiro SD, 2003, EUR RESPIR J, V22, p30S, DOI 10.1183/09031936.03.00000903a
  69. Shapiro SD, 1999, AM J RESP CRIT CARE, V160, pS29, DOI 10.1164/ajrccm.160.supplement_1.9
  70. Sharafkhaneh Amir, 2008, Proc Am Thorac Soc, V5, P475, DOI 10.1513/pats.200708-126ET
  71. SOZZANI S, 1994, J IMMUNOL, V152, P3615
  72. Su X, 2010, J IMMUNOL, V184, P401, DOI 10.4049/jimmunol.0901808
  73. Taraseviciene-Stewart L, 2008, J CLIN INVEST, V118, P394, DOI 10.1172/JCI31811
  74. Tracey KJ, 2007, J CLIN INVEST, V117, P289, DOI 10.1172/JCI30555
  75. Vacca G, 2011, RESP RES, V12, DOI 10.1186/1465-9921-12-24
  76. Wang H, 2003, NATURE, V421, P384, DOI 10.1038/nature01339
  77. Wright JL, 2008, AM J PHYSIOL-LUNG C, V295, pL1, DOI [10.1152/ajplung.90200.2008, DOI 10.1152/ajplung.90200.2008]
  78. Zhang J, 2011, RESP RES, V12, DOI 10.1186/1465-9921-12-158

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/20
Artigos e Materiais de Revistas Científicas - ODS/03