Genetic description of VanD phenotype vanA genotype in vancomycin-resistant Enterococcus faecium isolates from a Bone Marrow Transplantation Unit

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art_MARCHI_Genetic_description_of_VanD_phenotype_vanA_genotype_in_2022.PDF (1.65 MB)
Citações na Scopus
1
Tipo de produção
article
Data de publicação
2022
DOI
Scopus
Web of Science
PubMed
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ISSN da Revista
Título do Volume
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SPRINGER
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Citação
BRAZILIAN JOURNAL OF MICROBIOLOGY, v.53, n.1, p.245-250, 2022
Projetos de Pesquisa
Unidades Organizacionais
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Resumo
Background Vancomycin-resistant Enterococcus faecium (VREfm) is an important agent of hospital-acquired infection. VanA phenotype is characterized by resistance to high levels of vancomycin and teicoplanin and is encoded by the vanA gene, whereas VanD phenotype is characterized by resistance to vancomycin and susceptibility or intermediate resistance to teicoplanin; however, some isolates carry a VanD phenotype with a vanA genotype, but there are many gaps in the knowledge about the genetic mechanisms behind this pattern. Objective To characterize the genetic structure, clonality, and mobile genetic elements of VRE isolates that display a VanD-vanA phenotype. Results All vanA VRE-fm isolates displayed minimum inhibitory concentration (MIC) for vancomycin > 32 mu g/mL and intermediate or susceptible MIC range for teicoplanin (8-16 mu g/mL). The isolates were not clonal, and whole-genome sequencing analysis showed that they belonged to five different STs (ST478, ST412, ST792, ST896, and ST1393). The absence of some van complex genes were observed in three isolates: Ef5 lacked vanY and vanZ, Ef2 lacked vanY, and Ef9 lacked orf1 and orf2; moreover, another three isolates had inverted positions of orf1, orf2, vanR, and vanS genes. IS1542 was observed in all isolates, whereas IS1216 in only five. Moreover, presence of other hypothetical protein-encoding genes located downstream the vanZ gene were observed in six isolates. Conclusion VRE isolates can display some phenotypes associated to vanA genotype, including VanA and VanB, as well as VanD; however, further studies are needed to understand the exact role of genetic variability, rearrangement of the transposon Tn1546, and presence of insertion elements in isolates with this profile.
Palavras-chave
Enterococcus faecium, Whole-genome sequence, Resistance, Molecular characterization
URI
https://observatorio.fm.usp.br/handle/OPI/45877
Referências
  1. ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2
  2. Bogaty C, 2018, ANTIMICROB RESIST IN, V7, DOI 10.1186/s13756-018-0437-7
  3. Bortolaia V, 2020, J ANTIMICROB CHEMOTH, V75, P3491, DOI 10.1093/jac/dkaa345
  4. Camargo ILBC, 2006, J CLIN MICROBIOL, V44, P2635, DOI 10.1128/JCM.00509-06
  5. Cha JO, 2013, J APPL MICROBIOL, V115, P969, DOI 10.1111/jam.12300
  6. Clinical and Laboratory Standards Institute (CLSI), 2020, M100S30 CLSI
  7. Dalla Costa LM, 2000, ANTIMICROB AGENTS CH, V44, P3444, DOI 10.1128/AAC.44.12.3444-3446.2000
  8. Faria NA, 2008, J CLIN MICROBIOL, V46, P136, DOI 10.1128/JCM.01684-07
  9. Gagnon S, 2011, J ANTIMICROB CHEMOTH, V66, P2758, DOI 10.1093/jac/dkr379
  10. Joensen KG, 2014, J CLIN MICROBIOL, V52, P1501, DOI 10.1128/JCM.03617-13
  11. Kim D, 2020, ANTIMICROB AGENTS CH, V64, DOI 10.1128/AAC.02180-19
  12. Larsen MV, 2012, J CLIN MICROBIOL, V50, P1355, DOI 10.1128/JCM.06094-11
  13. Lee WG, 2004, ANTIMICROB AGENTS CH, V48, P1379, DOI 10.1128/AAC.48.4.1379-1381.2004
  14. Marchi AP, 2018, J HOSP INFECT, V99, P346, DOI 10.1016/j.jhin.2017.10.010
  15. Naas T, 2005, J CLIN MICROBIOL, V43, P3642, DOI 10.1128/JCM.43.8.3642-3649.2005
  16. Rothberg JM, 2011, NATURE, V475, P348, DOI 10.1038/nature10242
  17. Sato T, 2020, J ANTIMICROB CHEMOTH, V75, P2411, DOI 10.1093/jac/dkaa220
  18. Song JH, 2008, J ANTIMICROB CHEMOTH, V61, P838, DOI 10.1093/jac/dkn025
  19. Song JY, 2013, JPN J INFECT DIS, V66, P1
  20. Sullivan MJ, 2011, BIOINFORMATICS, V27, P1009, DOI 10.1093/bioinformatics/btr039
  21. Wardal E, 2017, EUR J CLIN MICROBIOL, V36, P313, DOI 10.1007/s10096-016-2804-8
  22. Zhou XW, 2020, ANTIMICROB RESIST IN, V9, DOI 10.1186/s13756-020-00770-1

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/31
Artigos e Materiais de Revistas Científicas - LIM/46
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