https://observatorio.fm.usp.br/handle/OPI/45925
Título: | Perivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinone |
Autor: | VIRGENS, Anangelica R.; GOES, Heliana F. O.; CARVALHO, Gabriel C. de; PIETROBON, Anna Julia; BRANCO, Anna Claudia C. C.; RAMOS, Yasmim A. L.; PEREIRA, Naiura V.; ORFALI, Raquel L.; AOKI, Valeria; SILVA, Luiz Fernando F. da; SOTTO, Mirian N.; REIS, Vitor M. S. dos; SATO, Maria N. |
Citación: | EXPERIMENTAL DERMATOLOGY, v.31, n.2, p.191-201, 2022 |
Resumen: | Background Methylisothiazolinone (MI) and Methylchloroisothiazolinone (MCI) are among the most common skin sensitizers, yet the immunological events that occur during MCI/MI allergic contact dermatitis (ACD) are still poorly understood. Objectives: To analyse dendrocytes, macrophage subtypes and T cells in skin during the elicitation phase of MCI/MI ACD. Methods Thirteen patients with positive patch test reactions to MCI/MI (ACD group) and 11 individuals with negative patch test results were selected. Skin biopsies were only performed at 48 hours of patch testing. Immunohistochemistry was conducted to assess T cells, dendrocytes (Factor XIIIa), M1 (p-Stat1, CD68) and M2 (c-Maf, CD163) macrophages. Transcriptional analyses were performed for cytokines and related factors, and further compared to atopic dermatitis samples (n=4). Immunofluorescence assays addressed T cells location, along with IL-4 or IL-13, within the skin. Results MCI/MI elicited dermal dendrocytes and macrophages, pronouncedly the M2 subtype. T cells, majorly CD4+ T cells, accumulated in the perivascular areas. Similarly, abundant IL-4 protein was detected in these areas. There was an upregulation of IL-4 and IL-13 mRNA expression, a mild increase in IFNG mRNA levels and a down-regulation of RORC in the ACD group. Immunofluorescence revealed dermal clusters of T cells co-localized with IL-4. Conclusions M2 macrophages and Th2 cells participate in the immunopathogenesis of MCI/MI ACD. Dermal dendrocytes and M2 macrophages may assist the formation of CD4+ T cells perivascular clusters. These findings render a mechanistic insight into the MCI/MI reaction. Further analysis at different timepoints of patch testing is required to fully comprehend this ACD kinetics. |
Aparece en las colecciones: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/05 Artigos e Materiais de Revistas Científicas - LIM/53 Artigos e Materiais de Revistas Científicas - LIM/56 Artigos e Materiais de Revistas Científicas - ODS/03 |
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