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https://observatorio.fm.usp.br/handle/OPI/46115
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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | NUCCI, Mariana P. | - |
dc.contributor.author | OLIVEIRA, Fernando A. | - |
dc.contributor.author | FERREIRA, Joao M. | - |
dc.contributor.author | PINTO, Yolanda O. | - |
dc.contributor.author | ALVES, Arielly H. | - |
dc.contributor.author | MAMANI, Javier B. | - |
dc.contributor.author | NUCCI, Leopoldo P. | - |
dc.contributor.author | VALLE, Nicole M. E. | - |
dc.contributor.author | GAMARRA, Lionel F. | - |
dc.date.accessioned | 2022-04-19T13:05:09Z | - |
dc.date.available | 2022-04-19T13:05:09Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | CELLS, v.11, n.3, article ID 485, 20p, 2022 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/46115 | - |
dc.description.abstract | The goal of this study is to see how combining physical activity with cell treatment impacts functional recovery in a stroke model. Molecular imaging and multimodal nanoparticles assisted in cell tracking and longitudinal monitoring (MNP). The viability of mesenchymal stem cell (MSC) was determined using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and bioluminescent image (BLI) after lentiviral transduction and MNP labeling. At random, the animals were divided into 5 groups (control-G1, and experimental G2-G5). The photothrombotic stroke induction was confirmed by local blood perfusion reduction and Triphenyltetrazolium chloride (TTC), and MSC in the G3 and G5 groups were implanted after 24 h, with BLI and near-infrared fluorescence image (NIRF) tracking these cells at 28 h, 2, 7, 14, and 28 days. During a 28-day period, the G5 also conducted physical training, whereas the G4 simply did the training. At 0, 7, 14, and 28 days, the animals were functionally tested using a cylinder test and a spontaneous motor activity test. MNP internalization in MSC was confirmed using brightfield and fluorescence microscopy. In relation to G1 group, only 3% of cell viability reduced. The G2-G5 groups showed more than 69% of blood perfusion reduction. The G5 group performed better over time, with a progressive recovery of symmetry and an increase of fast vertical movements. Up to 7 days, BLI and NIRF followed MSC at the damaged site, demonstrating a signal rise that could be connected to cell proliferation at the injury site during the acute phase of stroke. Local MSC therapy mixed with physical activity resulted in better results in alleviating motor dysfunction, particularly during the acute period. When it comes to neurorehabilitation, this alternative therapy could be a suitable fit. | eng |
dc.description.sponsorship | CNPqConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [465259/2014-6, 400856/2016-6] | - |
dc.description.sponsorship | FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2019/21070-3, 2017/17868-4, 2016/21470-3] | - |
dc.description.sponsorship | SisNANO 2.0/MCTIC [442539/2019-3] | - |
dc.description.sponsorship | National Institute of Science and Technology Complex Fluids (INCT-FCx) | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | eng |
dc.relation.ispartof | Cells | - |
dc.rights | openAccess | eng |
dc.subject | cell therapy | eng |
dc.subject | physical exercise | eng |
dc.subject | multimodal nanoparticles | eng |
dc.subject | stroke | eng |
dc.subject | behavioral test | eng |
dc.subject | human bone marrow mesenchymal stem cells | eng |
dc.subject | near-infrared fluorescence image | eng |
dc.subject | bioluminescence | eng |
dc.subject.other | mesenchymal stem-cells | eng |
dc.subject.other | iron-oxide nanoparticles | eng |
dc.subject.other | long-term tracking | eng |
dc.subject.other | neurogenesis | eng |
dc.subject.other | reperfusion | eng |
dc.subject.other | fluorescent | eng |
dc.subject.other | mechanisms | eng |
dc.subject.other | survival | eng |
dc.subject.other | efficacy | eng |
dc.title | Effect of Cell Therapy and Exercise Training in a Stroke Model, Considering the Cell Track by Molecular Image and Behavioral Analysis | eng |
dc.type | article | eng |
dc.rights.holder | Copyright MDPI | eng |
dc.identifier.doi | 10.3390/cells11030485 | - |
dc.identifier.pmid | 35159294 | - |
dc.subject.wos | Cell Biology | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | OLIVEIRA, Fernando A.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.author.external | FERREIRA, Joao M.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.author.external | PINTO, Yolanda O.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.author.external | ALVES, Arielly H.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.author.external | MAMANI, Javier B.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.author.external | NUCCI, Leopoldo P.:Ctr Univ Planalto Cent, BR-72445020 Brasilia, DF, Brazil | - |
hcfmusp.author.external | VALLE, Nicole M. E.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.author.external | GAMARRA, Lionel F.:Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo, Brazil | - |
hcfmusp.description.articlenumber | 485 | - |
hcfmusp.description.issue | 3 | - |
hcfmusp.description.volume | 11 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000754862300001 | - |
hcfmusp.origem.id | 2-s2.0-85123522627 | - |
hcfmusp.publisher.city | BASEL | eng |
hcfmusp.publisher.country | SWITZERLAND | eng |
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dc.description.index | MEDLINE | eng |
dc.identifier.eissn | 2073-4409 | - |
hcfmusp.citation.scopus | 6 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/Outros Artigos e Materiais de Revistas Científicas - LIM/44 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_NUCCI_Effect_of_Cell_Therapy_and_Exercise_Training_in_2022.PDF | publishedVersion (English) | 2.49 MB | Adobe PDF | View/Open |
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