Surface Topography Obtained with High Throughput Technology for hiPSC-Derived Cardiomyocyte Conditioning

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Citações na Scopus
0
Tipo de produção
conferenceObject
Data de publicação
2022
DOI
Scopus
Web of Science
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER
Autores
S, M.
LASAGNI, A. F.
Citação
XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020, p.119-126, 2022
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) to replace myocardial tissue after an infarct holds great promises. However, hiPSC-CM are phenotypically immature when compared to cells in the adult heart, hampering their clinical application. We aimed to develop and test a surface structuring technique that would improve hiPSC-CM structural maturation. Laser ablation was used to fabricate a micron-pattern on polyurethane surface and evaluated cell morphology, orientation and F-actin assemblage to detect phenotypic changes in response to the microtopography. This topography positively influenced cell morphology regarding to spreading area and elongation, and hiPSC-CM orientation, improving their structural maturation. The methodology thus presented has relatively low cost and is easily scalable, making it relevant for high-throughput applications such as drug screening for the pharma industry.
Palavras-chave
hiPSC-CM, Cardiomyocytes, Surface topography, Direct laser interference patterning, Polyurethane
URI
https://observatorio.fm.usp.br/handle/OPI/50344
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Coleções
Comunicações em Eventos - HC/InCor
Comunicações em Eventos - HC/IOT
Comunicações em Eventos - LIM/65