The Exometabolome of <i>Xylella fastidiosa</i> in Contact with <i>Paraburkholderia phytofirmans</i> Supernatant Reveals Changes in Nicotinamide, Amino Acids, Biotin, and Plant Hormones

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Tipo de produção
article
Data de publicação
2024
DOI
Scopus
Web of Science
PubMed
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Título da Revista
ISSN da Revista
Título do Volume
Editora
MDPI
Autores
FEITOSA-JUNIOR, Oseias R.
LUBBE, Andrea
KOSINA, Suzanne M.
MARTINS-JUNIOR, Joaquim
BACCARI, Clelia
ZAINI, Paulo A.
BOWEN, Benjamin P.
NORTHEN, Trent R.
LINDOW, Steven E.
Citação
METABOLITES, v.14, n.2, article ID 82, 26p, 2024
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Microbial competition within plant tissues affects invading pathogens' fitness. Metabolomics is a great tool for studying their biochemical interactions by identifying accumulated metabolites. Xylella fastidiosa, a Gram-negative bacterium causing Pierce's disease (PD) in grapevines, secretes various virulence factors including cell wall-degrading enzymes, adhesion proteins, and quorum-sensing molecules. These factors, along with outer membrane vesicles, contribute to its pathogenicity. Previous studies demonstrated that co-inoculating X. fastidiosa with the Paraburkholderia phytofirmans strain PsJN suppressed PD symptoms. Here, we further investigated the interaction between the phytopathogen and the endophyte by analyzing the exometabolome of wild-type X. fastidiosa and a diffusible signaling factor (DSF) mutant lacking quorum sensing, cultivated with 20% P. phytofirmans spent media. Liquid chromatography-mass spectrometry (LC-MS) and the Method for Metabolite Annotation and Gene Integration (MAGI) were used to detect and map metabolites to genomes, revealing a total of 121 metabolites, of which 25 were further investigated. These metabolites potentially relate to host adaptation, virulence, and pathogenicity. Notably, this study presents the first comprehensive profile of X. fastidiosa in the presence of a P. phytofirmans spent media. The results highlight that P. phytofirmans and the absence of functional quorum sensing affect the ratios of glutamine to glutamate (Gln:Glu) in X. fastidiosa. Additionally, two compounds with plant metabolism and growth properties, 2-aminoisobutyric acid and gibberellic acid, were downregulated when X. fastidiosa interacted with P. phytofirmans. These findings suggest that P. phytofirmans-mediated disease suppression involves modulation of the exometabolome of X. fastidiosa, impacting plant immunity.
Palavras-chave
Xylella fastidiosa, Paraburkholderia phytofirmans, metabolomics, phytopathogen, liquid chromatography-mass spectrometry, MAGI
URI
https://observatorio.fm.usp.br/handle/OPI/59296
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor