Association of a variant in the regulatory region of NADPH oxidase 4 gene and metabolic syndrome in patients with chronic hepatitis C

Imagem de Miniatura
Arquivos
art_SIQUEIRA_Association_of_a_variant_in_the_regulatory_region_2015.PDF (404.32 KB)
Citações na Scopus
5
Tipo de produção
article
Data de publicação
2015
DOI
Scopus
Web of Science
Título da Revista
ISSN da Revista
Título do Volume
Editora
BIOMED CENTRAL LTD
Autores
VASCONCELOS, Luydson Richardson Silva
CARMO, Rodrigo Feliciano
PEREIRA, Leila Maria Moreira Beltrao
Citação
EUROPEAN JOURNAL OF MEDICAL RESEARCH, v.20, article ID 45, 6p, 2015
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Given the important contribution of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system to the generation of reactive oxygen species induced by hepatitis C virus (HCV), we investigated two single nucleotide polymorphisms (SNPs) in the putative regulatory region of the genes encoding NADPH oxidase 4 catalytic subunit (NOX4) and its regulatory subunit p22phox (CYBA) and their relation with metabolic and histological variables in patients with HCV. Methods: One hundred seventy eight naive HCV patients (49.3% male; 65% HCV genotype 1) with positive HCV RNA were genotyped using specific primers and fluorescent-labeled probes for SNPs rs3017887 in NOX4 and -675 T -> A in CYBA. Results: No association was found between the genotype frequencies of NOX4 and CYBA SNPs and inflammation scores or fibrosis stages in the overall population. The presence of the CA + AA genotypes of the NOX4 SNP was nominally associated with a lower alanine aminotransferase (ALT) concentration in the male population (CA + AA = 72.23 +/- 6.34 U/L versus CC = 100.22 +/- 9.85; mean +/- SEM; P = 0.05). The TT genotype of the CYBA SNP was also nominally associated with a lower ALT concentration in the male population (TT = 84.01 +/- 6.77 U/L versus TA + AA = 109.67 +/- 18.37 U/L; mean +/- SEM; P = 0.047). The minor A-allele of the NOX4 SNP was inversely associated with the frequency of metabolic syndrome (MS) in the male population (odds ratio (OR): 0.15; 95% confidence interval (CI): 0.03 to 0.79; P = 0.025). Conclusions: The results suggest that the evaluated NOX4 and CYBA SNPs are not direct genetic determinants of fibrosis in HCV patients, but nevertheless NOX4 rs3017887 SNP could indirectly influence fibrosis susceptibility due to its inverse association with MS in male patients.
Palavras-chave
Hepatitis C, NADPH oxidase 4 polymorphisms, Metabolic syndrome
URI
https://observatorio.fm.usp.br/handle/OPI/9773
Referências
  1. Bedossa P, 1996, HEPATOLOGY, V24, P289, DOI 10.1002/hep.510240201
  2. Bureau C, 2001, J BIOL CHEM, V276, P23077, DOI 10.1074/jbc.M100698200
  3. Cave AC, 2006, ANTIOXID REDOX SIGN, V8, P691, DOI 10.1089/ars.2006.8.691
  4. Choi J, 2014, FREE RADICAL BIO MED, V72, P267, DOI 10.1016/j.freeradbiomed.2014.04.020
  5. de Mochel NSR, 2010, HEPATOLOGY, V52, P47, DOI 10.1002/hep.23671
  6. Elnakish MT, 2013, J PATHOL, V231, P290, DOI 10.1002/path.4255
  7. FRIEDEWA.WT, 1972, CLIN CHEM, V18, P499
  8. Gong GZ, 2001, P NATL ACAD SCI USA, V98, P9599, DOI 10.1073/pnas.171311298
  9. Grundy SM, 2004, ARTERIOSCL THROM VAS, V24, pE19, DOI 10.1161/01.ATV.0000112379.88385.67
  10. Guichard C, 2008, BIOCHEM SOC T, V36, P920, DOI 10.1042/BST0360920
  11. Kawaguchi Y, 2014, WORLD J GASTROENTERO, V20, P2888, DOI 10.3748/wjg.v20.i11.2888
  12. Lavanchy D, 2008, BEST PRACT RES CL GA, V22, P991, DOI 10.1016/j.bpg.2008.11.002
  13. Li QS, 2014, PLOS PATHOG, V10, DOI 10.1371/journal.ppat.1004163
  14. Lim SC, 2009, DIABETOLOGIA, V52, P1343, DOI 10.1007/s00125-009-1368-x
  15. Loguercio C, 2003, FREE RADICAL BIO MED, V34, P1, DOI 10.1016/S0891-5849(02)01167-X
  16. Meaney MJ, 2010, CHILD DEV, V81, P41, DOI 10.1111/j.1467-8624.2009.01381.x
  17. Monteiro MB, 2013, FREE RADICAL RES, V47, P804, DOI 10.3109/10715762.2013.828347
  18. Moreno MU, 2007, J HYPERTENS, V25, P1620, DOI 10.1097/HJH.0b013e3281ac211d
  19. Moriya K, 2001, CANCER RES, V61, P4365
  20. Okuda M, 2002, GASTROENTEROLOGY, V122, P366, DOI 10.1053/gast.2002.30983
  21. Pereira LMMB, 2013, BMC INFECT DIS, V13, DOI 10.1186/1471-2334-13-60
  22. Stuyver L, 1996, J CLIN MICROBIOL, V34, P2259
  23. Yu L, 2012, MOL BIOL REP, V39, P6641, DOI 10.1007/s11033-012-1469-3

Coleções
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/07
Artigos e Materiais de Revistas Científicas - LIM/25
Artigos e Materiais de Revistas Científicas - ODS/03