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https://observatorio.fm.usp.br/handle/OPI/13421
Title: | Lack of galectin-3 up-regulates IgA expression by peritoneal B1 lymphocytes during B cell differentiation |
Authors: | OLIVEIRA, Felipe L.; BERNARDES, Emerson S.; BRAND, Camila; SANTOS, Sofia N. dos; CABANEL, Mariana P.; ARCANJO, Katia D.; BRITO, Jose M.; BOROJEVIC, Radovan; CHAMMAS, Roger; EL-CHEIKH, Marcia C. |
Citation: | CELL AND TISSUE RESEARCH, v.363, n.2, p.411-426, 2016 |
Abstract: | Galectin-3 is a beta-galactoside-binding protein with an inhibitory role in B cell differentiation into plasma cells in distinct lymphoid tissues. We use a model of chronic schistosomiasis, a well-characterized experimental disease hallmarked by polyclonal B cell activation, in order to investigate the role of galectin-3 in controlling IgA production through peritoneal B1 cells. Chronically infected, galectin-3-deficient mice (Lgals3 (-/-)) display peritoneal fluid hypercellularity, increased numbers of atypical peritoneal IgM(+)/IgA(+) B1a and B1b lymphocytes and histological disturbances in plasma cell niches when compared with Lgals3 (+/+) mice. Similar to our infection model, peritoneal B1 cells from uninfected Lgals3 (-/-) mice show enhanced switching to IgA after in vitro treatment with interleukin-5 plus transforming growth factor-beta (IL-5 + TGF-beta 1). A higher number of IgA(+) B1a lymphocytes was found in the peritoneal cavity of Lgals3 (-/-)-uninfected mice at 1 week after i.p. injection of IL-5 + TGF-beta 1; this correlates with the increased levels of secreted IgA detected in the peritoneal fluid of these mice after cytokine treatment. Interestingly, a higher number of degranulated mast cells is present in the peritoneal cavity of uninfected and Schistosoma mansoni-infected Lgals3 (-/-) mice, indicating that, at least in part, mast cells account for the enhanced differentiation of B1 into IgA-producing B cells found in the absence of galectin-3. Thus, a novel role is revealed for galectin-3 in controlling the expression of surface IgA by peritoneal B1 lymphocytes; this might have important implications for manipulating the mucosal immune response. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDR Artigos e Materiais de Revistas Científicas - LIM/24 |
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