Glutathione Ethyl Ester Supplementation during Pancreatic Islet Isolation Improves Viability and Transplant Outcomes in a Murine Marginal Isleet Mass Model

Imagem de Miniatura
Arquivos
art_AMARAL_Glutathione_Ethyl_Ester_Supplementation_during_Pancreatic_Islet_Isolation_2013.PDF (654.57 KB)
Citações na Scopus
22
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
PAWLICK, Rena L.
PEPPER, Andrew
SHAPIRO, A. M. James
Citação
PLOS ONE, v.8, n.2, article ID e55288, 8p, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: The success of pancreatic islet transplantation still faces many challenges, mainly related to cell damage during islet isolation and early post-transplant. The increased generation of reactive oxygen species (ROS) during islet isolation and the consumption of antioxidant defenses appear to be an important pathway related to islet damage. Methodology/Principal Findings: In the present study we evaluated whether supplementation of glutathione-ethyl-ester (GEE) during islet isolation could improve islet viability and transplant outcomes in a murine marginal islet mass model. We also cultured human islets for 24 hours in standard CMRL media with or without GEE supplementation. Supplementation of GEE decreased the content of ROS in isolated islets, leading to a decrease in apoptosis and maintenance of islet viability. A higher percentage of mice transplanted with a marginal mass of GEE treated islets became euglycemic after transplant. The supplementation of 20 mM GEE in cultured human islets significantly reduced the apoptosis rate in comparison to untreated islets. Conclusions/Significance: GEE supplementation was able to decrease the apoptosis rate and intracellular content of ROS in isolated islets and might be considered a potential intervention to improve islet viability during the isolation process and maintenance in culture before islet transplantation.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/1765
Referências
  1. Anderson MF, 2004, NEUROSCI LETT, V354, P163, DOI 10.1016/j.neulet.2003.09.067
  2. Armann B, 2007, AM J TRANSPLANT, V7, P38, DOI 10.1111/j.1600-6143.2006.01577.x
  3. Avila J, 2005, AM J TRANSPLANT, V5, P2830, DOI 10.1111/j.1600-6143.2005.01109.x
  4. Avila JG, 2003, CELL TRANSPLANT, V12, P877
  5. Biolo G, 2007, CRIT CARE MED, V35, pS591, DOI 10.1097/01.CCM.0000278913.19123.13
  6. BLAUSTEIN A, 1989, CIRCULATION, V80, P1449
  7. Bottino R, 2004, DIABETES, V53, P2559, DOI 10.2337/diabetes.53.10.2559
  8. Davalli AM, 1996, DIABETES, V45, P1161, DOI 10.2337/diabetes.45.9.1161
  9. Dickinson DA, 2002, ANN NY ACAD SCI, V973, P488
  10. Emamaullee JA, 2006, DIABETES, V55, P1907, DOI 10.2337/db05-1254
  11. Eriksson O, 2009, AM J TRANSPLANT, V9, P2816, DOI 10.1111/j.1600-6143.2009.02844.x
  12. FUKUZAWA K, 1995, TRANSPLANTATION, V59, P6, DOI 10.1097/00007890-199501150-00002
  13. GRANKVIST K, 1981, BIOCHEM J, V199, P393
  14. GRATTAGLIANO I, 1995, J PHARMACOL EXP THER, V272, P484
  15. Ichii H, 2005, AM J TRANSPLANT, V5, P1635, DOI 10.1111/j.1600-6143.2005.00913.x
  16. Kevelaitis E, 1997, TRANSPLANTATION, V64, P660, DOI 10.1097/00007890-199708270-00022
  17. Kin T, 2010, ISLETS LANGERHANS, P791
  18. Kin T, 2011, TRANSPL P, V43, P3195, DOI 10.1016/j.transproceed.2011.10.003
  19. Lakhani SA, 2006, SCIENCE, V311, P847, DOI 10.1126/science.1115035
  20. Lepore DA, 2004, XENOTRANSPLANTATION, V11, P53, DOI 10.1111/j.1399-3089.2004.00082.x
  21. LEVY EJ, 1993, P NATL ACAD SCI USA, V90, P9171, DOI 10.1073/pnas.90.19.9171
  22. Li CY, 2002, AM J PHYSIOL-CELL PH, V282, pC227
  23. Littman ED, 1995, J SURG RES, V59, P694, DOI 10.1006/jsre.1995.1225
  24. McCall MD, 2011, ISLETS, V3, P144, DOI 10.4161/isl.3.4.15729
  25. Mysore TB, 2005, DIABETES, V54, P2109, DOI 10.2337/diabetes.54.7.2109
  26. NEUSCHWANDERTETRI BA, 1992, J CLIN INVEST, V89, P109, DOI 10.1172/JCI115550
  27. Pileggi A, 2006, ANN NY ACAD SCI, V1079, P383, DOI 10.1196/annals.1375.059
  28. PURI RN, 1983, P NATL ACAD SCI-BIOL, V80, P5258, DOI 10.1073/pnas.80.17.5258
  29. Robertson RP, 2004, DIABETES, V53, pS119, DOI 10.2337/diabetes.53.2007.S119
  30. Ryan EA, 2005, DIABETES, V54, P2060, DOI 10.2337/diabetes.54.7.2060
  31. Schauer RJ, 2004, ANN SURG, V239, P220, DOI 10.1097/01.sla.0000110321.64275.95
  32. Shapiro AMJ, 2000, NEW ENGL J MED, V343, P230, DOI 10.1056/NEJM200007273430401
  33. SOUTHARD JH, 1990, TRANSPLANTATION, V49, P251, DOI 10.1097/00007890-199002000-00004
  34. Sumimoto K, 1996, TRANSPLANTATION, V62, P1391, DOI 10.1097/00007890-199611270-00003
  35. Tonooka N, 2007, CLIN TRANSPLANT, V21, P767, DOI 10.1111/j.1399-0012.2007.00736.x
  36. YU WM, 1990, TRANSPLANTATION, V49, P1060, DOI 10.1097/00007890-199006000-00007
  37. Zeevalk GD, 2007, EXP NEUROL, V203, P512, DOI 10.1016/j.expneurol.2006.09.004

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/25
Artigos e Materiais de Revistas Científicas - LIM/37