A comparative study of extracellular matrix remodeling in two murine models of emphysema

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author LOPES, F. D. T. Q. S. FMUSP-HC
TOLEDO, A. C. FMUSP-HC
OLIVO, C. R. FMUSP-HC
PRADO, C. M.
LEICK, E. A. FMUSP-HC
MEDEIROS, M. C.
SANTOS, A. B. G. FMUSP-HC
GARIPPO, A. FMUSP-HC
MARTINS, M. A. FMUSP-HC
MAUAD, T. FMUSP-HC
dc.date.issued 2013
dc.identifier.citation HISTOLOGY AND HISTOPATHOLOGY, v.28, n.2, p.269-276, 2013
dc.identifier.issn 0213-3911
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/1773
dc.description.abstract A single instillation of porcine pancreatic elastase (PPE) results in significant airspace enlargement on the 28th day after instillation, whereas cigarette smoke (CS) exposure requires 6 months to produce mild emphysema in rodents. Considering that there are differences in the pathogenesis of parenchymal destruction in these different experimental models, it is likely that there may be different patterns of extracellular matrix (ECM) remodeling. To evaluate ECM remodeling, C57BL/6 mice were submitted to either a nasal drop of PPE (PPE 28 Days) or exposed for 6 months to cigarette smoke (CS 6 months). Control groups received either an intranasal instillation of saline solution (Saline 28 Days) or remained without any smoke inhalation for six months (Control 6 months). We measured the mean linear intercept and the volume proportion of collagen type I, collagen type III, elastin and fibrillin. We used emission-scanning confocal microscopy to verify the fiber distribution. Both models induced increased mean linear intercept in relation to the respective controls, being larger in the elastase model in relation to the CS model. In the CS model, emphysema was associated with an increase in the volume proportion of fibrillin, whereas in the PPE model there was an increase in the parenchymal elastin content. In both models, there was an increase in collagen type III, which was higher in the CS-exposed mice. We concluded that ECM remodeling is different in the two most used experimental models of emphysema.
dc.language.iso eng
dc.publisher F HERNANDEZ
dc.relation.ispartof Histology and Histopathology
dc.rights restrictedAccess
dc.subject Emphysema; Extracellular matrix remodeling; Elastin; Fibrillin-1
dc.subject.other obstructive pulmonary-disease; elastase-induced emphysema; cigarette-smoke; mechanical forces; global strategy; animal-models; lung; collagen; expression; prevention
dc.title A comparative study of extracellular matrix remodeling in two murine models of emphysema
dc.type article
dc.rights.holder Copyright F HERNANDEZ
dc.description.group LIM/20
dc.description.group LIM/59
dc.description.group LIM/05
dc.identifier.pmid 23275309
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author LOPES, F. D. T. Q. S.:HC:LIM/20
hcfmusp.author TOLEDO, A. C.:FM:
hcfmusp.author OLIVO, C. R.:HC:ICHC
hcfmusp.author LEICK, E. A.:FM:MPT
hcfmusp.author SANTOS, A. B. G.:HC:LIM/59
hcfmusp.author GARIPPO, A.:FM:MPR
hcfmusp.author MARTINS, M. A.:FM:MCM
hcfmusp.author MAUAD, T.:FM:MPT
hcfmusp.author.external · PRADO, C. M.:Univ Fed Sao Paulo, Dept Biol Sci, Sao Paulo, Brazil
hcfmusp.origem.id 2-s2.0-84873826174
hcfmusp.origem.id WOS:000312935200011
hcfmusp.publisher.city MURCIA
hcfmusp.publisher.country SPAIN
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dc.description.index MEDLINE
hcfmusp.citation.scopus 21
hcfmusp.affiliation.country Brasil
hcfmusp.scopus.lastupdate 2021-08-27


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