DNA methylation pattern changes following a short-term hypocaloric diet in women with obesity

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art_NICOLETTI_DNA_methylation_pattern_changes_following_a_shortterm_hypocaloric_2020.PDF (1.77 MB)
Citações na Scopus
14
Tipo de produção
article
Data de publicação
2020
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
NATURE PUBLISHING GROUP
Autores
NICOLETTI, C. F.
CORTES-OLIVEIRA, C.
NORONHA, N. Y.
PINHEL, M. A. S.
JACOME, A.
MARCHINI, J. S.
CRUJEIRAS, A. B.
NONINO, C. B.
Citação
EUROPEAN JOURNAL OF CLINICAL NUTRITION, v.74, n.9, p.1345-1353, 2020
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background/Objectives We aimed to investigate the effects of short-term hypocaloric diet-induced weight loss on DNA methylation profile in leukocytes from women with severe obesity. Methods Eleven women with morbid obesity (age: 36.9 +/- 10.3 years; BMI: 58.5 +/- 10.5 kg/m(2)) were assessed before and after 6 weeks of a hypocaloric dietary intervention. The participants were compared with women of average weight and the same age (age: 36.9 +/- 11.8 years; BMI: 22.5 +/- 1.6 kg/m(2)). Genome-wide DNA methylation analysis was performed in DNA extracted from peripheral blood leukocytes using the Infinium Human Methylation 450 BeadChip assay. Changes (Delta beta) in the methylation level of each CpGs were calculated. A threshold with a minimum value of 10%, p < 0.001, for the significant CpG sites based on Delta beta and a false discovery rate of Results Dietary intervention changed the methylation levels at 16,064 CpG sites. These CpGs sites were related to cancer, cell cycle-related, MAPK, Rap1, and Ras signaling pathways. However, regardless of hypocaloric intervention, a group of 878 CpGs (related to 649 genes) remained significantly altered in obese women when compared with normal-weight women. Pathway enrichment analysis identified genes related to the cadherin and Wnt pathway, angiogenesis signaling, and p53 pathways by glucose deprivation. Conclusion A short-term hypocaloric intervention in patients with severe obesity partially restored the obesity-related DNA methylation pattern. Thus, the full change of obesity-related DNA methylation patterns could be proportional to the weight-loss rate in these patients after dietary interventions.
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URI
https://observatorio.fm.usp.br/handle/OPI/37656
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - LIM/17
Artigos e Materiais de Revistas Científicas - ODS/03