Lung Edema and Mortality Induced by Intestinal Ischemia and Reperfusion Is Regulated by VAChT Levels in Female Mice
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Citações na Scopus
2
Tipo de produção
article
Data de publicação
2021
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER/PLENUM PUBLISHERS
Autores
SANTANA, Fernanda P. R.
FANTOZZI, Evelyn T.
PRADO, Marco Antonio M.
PRADO, Vania F.
TAVARES-DE-LIMA, Wothan
Citação
INFLAMMATION, v.44, n.4, p.1553-1564, 2021
Resumo
Acute lung injury induced by intestinal ischemia/reperfusion (I/R) is a relevant clinical condition. Acetylcholine (ACh) and the alpha 7 nicotinic ACh receptor (nAChR alpha-7) are involved in the control of inflammation. Mice with reduced levels of the vesicular ACh transporter (VAChT), a protein responsible for controlling ACh release, were used to test the involvement of cholinergic signaling in lung inflammation due to intestinal I/R. Female mice with reduced levels of VAChT (VAChT-KDHOM) or wild-type littermate controls (WT) were submitted to intestinal I/R followed by 2 h of reperfusion. Mortality, vascular permeability, and recruitment of inflammatory cells into the lung were investigated. Parts of mice were submitted to ovariectomy (OVx) to study the effect of sex hormones or treated with PNU-282,987 (nAChR alpha-7 agonist). A total of 43.4% of VAChT-KDHOM-I/R mice died in the reperfusion period compared to 5.2% of WT I/R mice. The I/R increased lung inflammation in both genotypes. In VAChT-KDHOM mice, I/R increased vascular permeability and decreased the release of cytokines in the lung compared to WT I/R mice. Ovariectomy reduced lung inflammation and permeability compared to non-OVx, but it did not avoid mortality in VAChT-KDHOM-I/R mice. PNU treatment reduced lung permeability, increased the release of proinflammatory cytokines and the myeloperoxidase activity in the lungs, and prevented the increased mortality observed in VAChT-KDHOM mice. Cholinergic signaling is an important component of the lung protector response against intestinal I/R injury. Decreased cholinergic signaling seems to increase pulmonary edema and dysfunctional cytokine release that increased mortality, which can be prevented by increasing activation of nAChR alpha-7.
Palavras-chave
acute lung injury, experimental model, acetylcholine, PNU-282,987, cholinergic anti-inflammatory pathway, intestinal ischemia and reperfusion
Referências
- Adib-Conquy M, 2000, AM J RESP CRIT CARE, V162, P1877, DOI 10.1164/ajrccm.162.5.2003058
- Aggarwal NR, 2014, AM J PHYSIOL-LUNG C, V306, pL709, DOI 10.1152/ajplung.00341.2013
- Bernik TR, 2002, J EXP MED, V195, P781, DOI 10.1084/jem.20011714
- Biswas SK, 2009, TRENDS IMMUNOL, V30, P475, DOI 10.1016/j.it.2009.07.009
- Boomer JS, 2011, JAMA-J AM MED ASSOC, V306, P2594, DOI 10.1001/jama.2011.1829
- Borovikova LV, 2000, NATURE, V405, P458
- Bosmans G, 2017, FRONT IMMUNOL, V8, DOI 10.3389/fimmu.2017.01873
- Bouras M, 2018, FRONT IMMUNOL, V9, DOI 10.3389/fimmu.2018.02590
- Breithaupt-Faloppa AC, 2013, SHOCK, V40, P203, DOI 10.1097/SHK.0b013e3182a01e24
- Cavaillon JM, 2006, CRIT CARE, V10, DOI 10.1186/cc5055
- Cavriani G, 2005, SHOCK, V23, P330, DOI 10.1097/01.shk.0000157303.76749.9b
- Cribbs SK, 2020, PHYSIOL REV, V100, P603, DOI 10.1152/physrev.00039.2018
- de Castro BM, 2009, MOL CELL BIOL, V29, P5238, DOI 10.1128/MCB.00245-09
- Fantozzi ET, 2018, J SURG RES, V221, P1, DOI 10.1016/j.jss.2017.07.038
- Feng XT, 2021, IMMUNOL CELL BIOL, V99, P206, DOI 10.1111/imcb.12400
- Gahring LC, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0175367
- Galle-Treger L, 2016, NAT COMMUN, V7, DOI 10.1038/ncomms13202
- Geha M, 2017, J IMMUNOL, V199, P2921, DOI 10.4049/jimmunol.1700655
- Gwilt CR, 2007, PHARMACOL THERAPEUT, V115, P208, DOI 10.1016/j.pharmthera.2007.05.007
- He Y, 2016, J SURG RES, V201, P258, DOI 10.1016/j.jss.2015.10.046
- Hotchkiss RS, 2013, LANCET INFECT DIS, V13, P260, DOI 10.1016/S1473-3099(13)70001-X
- Iida T, 2015, DIGESTION, V92, P211, DOI 10.1159/000439300
- Jana B, 2018, ANN ANAT, V216, P135, DOI 10.1016/j.aanat.2017.11.010
- Lips KS, 2007, LIFE SCI, V80, P2263, DOI 10.1016/j.lfs.2007.01.026
- Maca J, 2017, RESP CARE, V62, P113, DOI 10.4187/respcare.04716
- Norling LV, 2020, P NATL ACAD SCI USA, V117, P9148, DOI 10.1073/pnas.2004241117
- Oldenburg WA, 2004, ARCH INTERN MED, V164, P1054, DOI 10.1001/archinte.164.10.1054
- Parrish WR, 2008, MOL MED, V14, P567, DOI 10.2119/2008-00079.Parrish
- Pereira LM, 2018, BRAIN RES BULL, V140, P411, DOI 10.1016/j.brainresbull.2018.01.012
- Pinheiro NM, 2017, FASEB J, V31, P320, DOI 10.1096/fj.201600431R
- Pinheiro NM, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0120441
- Prado VF, 2006, NEURON, V51, P601, DOI 10.1016/j.neuron.2006.08.005
- Ren C, 2018, INT J BIOL SCI, V14, P748, DOI 10.7150/ijbs.24576
- Ricardo-da-Silva FY, 2017, SHOCK, V48, P477, DOI 10.1097/SHK.0000000000000873
- Righetti RF, 2018, FRONT PHARMACOL, V9, DOI 10.3389/fphar.2018.01021
- Rosas-Ballina M, 2011, SCIENCE, V334, P98, DOI 10.1126/science.1209985
- Roy A, 2013, FASEB J, V27, P5072, DOI 10.1096/fj.13-238279
- Santana FPR, 2019, ECOTOX ENVIRON SAFE, V167, P494, DOI 10.1016/j.ecoenv.2018.10.005
- Sekheri M, 2020, P NATL ACAD SCI USA, V117, P7971, DOI 10.1073/pnas.1920193117
- Su X, 2010, J IMMUNOL, V184, P401, DOI 10.4049/jimmunol.0901808
- Tendler David A, 2003, Semin Gastrointest Dis, V14, P66
- Tomlinson GS, 2014, J INFECT DIS, V209, P1055, DOI 10.1093/infdis/jit621
- Ward PA, 2012, EMBO MOL MED, V4, P1234, DOI 10.1002/emmm.201201375
- Xiao XM, 2004, J PEDIATR SURG, V39, P1828, DOI 10.1016/j.jpedsurg.2004.08.028
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Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - FM/MCP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/11
Carregar mais Artigos e Materiais de Revistas Científicas - FM/MCP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/11