Protoporphyrin fluorescence induced by methyl-ALA in skin healing

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art_GONDIM_Protoporphyrin_fluorescence_induced_by_methyl_ALA_in_skin_2013.PDF (2.12 MB)
Citações na Scopus
4
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE BV
Autores
VIEIRA, Vinicius C. C.
BAPTISTA, Mauricio S.
Citação
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, v.10, n.4, p.389-398, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Estimation of the time period that precedes an injury is critical in forensic medicine. However, there is no reliable method that can be used to evaluate the oldness of a lesion. The aim of this work is to develop a fluorimetric method that can be used to follow the aging process of lesions by applying methyl ALA (MAL) on wounds and by quantifying protoporphyrin IX (PPIX) fluorescence during the healing process. We also aim to understand the changes in PPIX fluorescence by establishing a correlation with histological evaluations during the heating process. Methods: Standardized linear wounds were made on the dorsum of 72 mice, which were divided in control (MAL -) and experimental (MAL +) groups. In vivo fluorescence spectra (FS) were collected from normal and wound skin sites of control and experimental groups, corresponding to four groups of FS spectra: (a) FS of skin wound after MAL (+/+); (b) FS of normal skin after MAL (-/+); (c) FS of skin wound without MAL (+/-) and (d) FS of normal skin without MAL (-/-). Animals were monitored periodically for 3 months and euthanized. Tissue specimens were processed for histological analysis using design-based stereological methods. Serial cross-sections were analyzed to evaluate the organization of the dermis and epidermis, collagen deposition and cellular proliferation. Results: FS of skin wound with MAL (+/+) showed an expressive intensity increase from the beginning of the experiment to the 34th day, with maximum fluorescence being observed on the similar to 11th day after wounding. There was preferential PPIX accumulation in healing sites as compared to adjacent normal skin (+/-) in the early stage of healing. Histological findings allowed correlation of the fluorescence increase mainly with cell proliferation. The drastic decrease in the FS intensity observed in the end of the healing process was correlated with the decrease in the proliferation rate as well as with the presence of new extracellular fibrous materials. Conclusions: In the mice wound-healing model tested here, it was possible to distinguish whether the injury was in early or advanced stages by using PPIX fluorescence induced by MAL. We conclude that this method is a promising approach to evaluate the age of skin wounding and we hope this work will stimulate human studies to allow this technique to become standardized in forensic medicine.
Palavras-chave
Spectroscopy, Lesion age, Traumatic lesion, Experimental surgery, Dermatology, Legal medicine
URI
https://observatorio.fm.usp.br/handle/OPI/5178
Referências
  1. Angell-Petersen E, 2007, BRIT J DERMATOL, V156, P301, DOI 10.1111/j.1365-2133.2006.07638.x
  2. Bacci S, 2006, INT J LEGAL MED, V120, P138, DOI 10.1007/s00414-005-0030-5
  3. Bourre L, 2008, MOL CANCER THER, V7, P1720, DOI 10.1158/1535-7163.MCT-08-0092
  4. Bracaleon L, 2001, PHOTOCHEM PHOTOBIOL, V73, P178
  5. Bruijin HS, 2008, J PHOTOCH PHOTOBIO B, V92, P91
  6. Bugaj A, 2006, J PHOTOCH PHOTOBIO B, V83, P94, DOI 10.1016/j.jphotobiol.2005.12.003
  7. Casas A, 2009, J CONTROL RELEASE, V135, P136, DOI 10.1016/j.jconrel.2009.01.002
  8. De Rosa FS, 2004, PHARM RES, V21, P2247, DOI 10.1007/s11095-004-7677-3
  9. Dimitrow E, 2009, EXP DERMATOL, V18, P509, DOI 10.1111/j.1600-0625.2008.00815.x
  10. Dognitz N, 2008, J PHOTOCH PHOTOBIO B, V93, P140, DOI 10.1016/j.jphotobiol.2008.07.012
  11. Gambichler T, 2008, PHOTODERMATOL PHOTO, V24, P67, DOI 10.1111/j.1600-0781.2008.00335.x
  12. Gillies R, 2000, J INVEST DERMATOL, V115, P704, DOI 10.1046/j.1523-1747.2000.00091.x
  13. Grellner W, 2005, FORENSIC SCI INT, V153, P174, DOI 10.1016/j.forsciint.2004.08.021
  14. GUNDERSEN HJG, 1981, J MICROSC-OXFORD, V121, P65
  15. Haedersdal M, 2011, LASER SURG MED, V43, P804, DOI 10.1002/lsm.21096
  16. Howard CV, 2005, UNBIASED STEREOLOGY, P103
  17. JUNQUEIRA LCU, 1979, HISTOCHEM J, V11, P447, DOI 10.1007/BF01002772
  18. Kirkpatrick ND, 2005, PHOTOCHEM PHOTOBIOL, V81, P125, DOI 10.1562/2004-08-09-RA-267.1
  19. Kleinpenning MM, 2006, BRIT J DERMATOL, V155, P539, DOI 10.1111/j.1365-2133.2006.07341.x
  20. Langer S, 2002, ANN PLAS SURG, V48, P646, DOI 10.1097/00000637-200206000-00014
  21. Liu NG, 2006, FORENSIC SCI INT, V162, P178, DOI 10.1016/j.forsciint.2006.06.040
  22. Mokry M, 2006, PHOTOCHEM PHOTOBIOL, V82, P793, DOI 10.1562/2005-09-08-RA-678
  23. Na RH, 2000, SKIN RES TECHNOL, V6, P112, DOI 10.1034/j.1600-0846.2000.006003112.x
  24. Nahm WK, 2004, J CELL PHYSIOL, V200, P309, DOI 10.1002/jcp.20010
  25. Ninomiya T, 2001, J DERMATOLOGICAL SCI, V27, P114
  26. Oehmichen M, 2004, FORENSIC SCI INT, V144, P221, DOI 10.1016/j.forsciint.2004.04.057
  27. Pan YA, 2004, J INVEST DERMATOL, V123, P888, DOI 10.1111/j.0022-202X.2004.23486.x
  28. Papadopulos F, 2007, ULTRASTRUCT PATHOL, V31, P401, DOI 10.1080/01913120701719189
  29. Payne G, 2007, MED SCI LAW, V47, P225
  30. Peng Q, 2001, J PHOTOCH PHOTOBIO B, V62, P140, DOI 10.1016/S1011-1344(01)00173-7
  31. Psaroudakis K, 2001, AM J FOREN MED PATH, V22, P341, DOI 10.1097/00000433-200112000-00002
  32. Randeberg LL, 2006, LASER SURG MED, V38, P277, DOI 10.1002/lsm.20301
  33. Sakamoto FH, 2010, J AM ACAD DERMATOL, V63, P183, DOI 10.1016/j.jaad.2009.09.056
  34. Sakamoto FH, 2011, LASER SURG MED, V43, P621, DOI 10.1002/lsm.21073
  35. Sharfaei S, 2002, ARCH DERMATOL RES, V294, P237, DOI 10.1007/s00403-002-0320-4
  36. Singer AJ, 1999, NEW ENGL J MED, V341, P738
  37. Smits T, 2005, J INVEST DERMATOL, V125, P833, DOI 10.1111/j.0022-202X.2005.23843.x
  38. Sorg H, 2007, J ANAT, V211, P810, DOI 10.1111/j.1469-7580.2007.00822.x
  39. Stenquist B, 2006, BRIT J DERMATOL, V154, P305, DOI 10.1111/j.1365-2133.2005.07035.x
  40. Stephenson T, 1996, ARCH DIS CHILD, V74, P53
  41. Takamiya M, 2005, INT J LEGAL MED, V119, P16, DOI 10.1007/s00414-004-0453-4
  42. Vena FCB, 2004, LASER MED SCI, V19, P119, DOI 10.1007/s10103-004-0313-y
  43. Weingarten MS, 2008, WOUND REPAIR REGEN, V16, P234, DOI 10.1111/j.1524-475X.2008.00364.x
  44. Wetzig T, 2010, BRIT J DERMATOL, V162, P1370, DOI 10.1111/j.1365-2133.2010.09666.x

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - FM/MLS
Artigos e Materiais de Revistas Científicas - LIM/25
Artigos e Materiais de Revistas Científicas - LIM/40
Artigos e Materiais de Revistas Científicas - LIM/59