Bovine-associated staphylococci and mammaliicocci trigger T-lymphocyte proliferative response and cytokine production differently

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art_SOUZA_Bovineassociated_staphylococci_and_mammaliicocci_trigger_Tlymphocyte_proliferative_response_2023.PDF (1.77 MB)
Citações na Scopus
2
Tipo de produção
article
Data de publicação
2023
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE INC
Autores
SOUZA, Fernando N.
SANTOS, Kamila R.
FERRONATTO, Jose A.
TOLEDO-SILVA, Bruno
HEINEMANN, Marcos B.
VLIEGHER, Sarne De
LIBERA, Alice M. M. P. Della
Citação
JOURNAL OF DAIRY SCIENCE, v.106, n.4, p.2772-2783, 2023
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
We examined whether distinct staphylococcal and mammaliicoccal species and strains trigger B-and T-lymphocyte proliferation and interleukin (IL)-17A and interferon (IFN)-gamma production by peripheral blood mononuclear cells in nulliparous, primiparous, and multiparous dairy cows. Flow cytometry was used to measure lymphocyte proliferation with the Ki67 anti-body, and specific monoclonal antibodies were used to identify CD3, CD4, and CD8 T lymphocyte and CD21 B lymphocyte populations. The supernatant of the peripheral blood mononuclear cell culture was used to measure IL-17A and IFN-gamma production. Two distinct, inactivated strains of bovine-associated Staphylococcus aureus [one causing a persistent intramammary infec-tion (IMI) and the other from the nose], 2 inactivated Staphylococcus chromogenes strains [one causing an IMI and the other from a teat apex), as well as an inactivated Mammaliicoccus fleurettii strain originat-ing from sawdust from a dairy farm, and the mitogens concanavalin A and phytohemagglutinin M-form (both specifically to measure lymphocyte proliferation) were studied. In contrast to the ""commensal"" Staph. aureus strain originating from the nose, the Staph. aureus strain causing a persistent IMI triggered proliferation of CD4+ and CD8+ subpopulations of T lymphocytes. The M. fleurettii strain and the 2 Staph. chromogenes strains had no effect on T-or B-cell proliferation. Fur-thermore, both Staph. aureus and Staph. chromogenes strains causing persistent IMI significantly increased IL-17A and IFN-gamma production by peripheral blood mononuclear cells. Overall, multiparous cows tended to have a higher B-lymphocyte and a lower T-lymphocyte proliferative response than primiparous and nulliparous cows. Peripheral blood mononuclear cells of multipa-rous cows also produced significantly more IL-17A and IFN-gamma. In contrast to concanavalin A, phytohemagglu-tinin M-form selectively stimulated T-cell proliferation.
Palavras-chave
mastitis, Staphylococcus aureus, non-aureus staphylococci, Mammaliicoccus, lymphocyte proliferation
URI
https://observatorio.fm.usp.br/handle/OPI/54293
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Coleções
Artigos e Materiais de Revistas Científicas - LIM/38