The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
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Citações na Scopus
1
Tipo de produção
article
Data de publicação
2019
Título da Revista
ISSN da Revista
Título do Volume
Editora
ACTA CIRURGICA BRASILEIRA
Autores
COGLIATI, Bruno
HOMEM, Adriana Ribeiro
ALOIAV, Thiago Pinheiro Arrais
AQUINO NETO, Nelson de
MOREIRA, Jairo Marques
RENO, Leonardo da Cruz
Citação
ACTA CIRURGICA BRASILEIRA, v.34, n.10, article ID e201901003, 9p, 2019
Resumo
Purpose: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. Methods: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. Results: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. Conclusions: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.
Palavras-chave
GAP junctions, Connexin 43, Ischemia, Reperfusion, Liver, Cell communication, Mice
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