https://observatorio.fm.usp.br/handle/OPI/8405
Título: | TGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding |
Autor: | ALVES, Claudia Cristina; TORRINHAS, Raquel Susana; GIORGI, Ricardo; BRENTANI, Maria Mitzi; LOGULLO, Angela Flavia; WAITZBERG, Dan Linetzky |
Citación: | INTERNATIONAL WOUND JOURNAL, v.11, n.5, p.533-539, 2014 |
Resumen: | Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. In experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets. |
Aparece en las colecciones: | Artigos e Materiais de Revistas Científicas - FM/MDR Artigos e Materiais de Revistas Científicas - FM/MFT Artigos e Materiais de Revistas Científicas - FM/MGT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/24 Artigos e Materiais de Revistas Científicas - LIM/25 Artigos e Materiais de Revistas Científicas - LIM/35 Artigos e Materiais de Revistas Científicas - ODS/02 |
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